Response Surface Methodology to Optimize a Bioprocess for Kefiran Production

Pais-Chanfrau, José Manuel; o, Luis Enrique Trujillo To led; Cóndor, Paola M. Alvarado; CuaranGuerrero, Milton Jimmy; Núñez-Pérez, Jimmy; Intriago, Leonor Margarita Rivera

doi:10.4172/0032-745x.1000285

Cited by 2 publications

(7 citation statements)

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“…For its simplicity, a central composite design was selected, bringing as a central point 25°C and 42% (m/m), for temperature and WL, respectively. A temperature of 25°C brings a maximum value of kefiran concentration in previous work with the same origin kefir grains ("Yogurt-Kombucha-Tibicos en Ecuador," Quito, Pichincha, Ecuador, www.kefir.ec), but employing whey powder as a lactose source [37].…”

Section: Introductionmentioning

confidence: 93%

“…The soluble exopolysaccharide kefiran, synthetized by Lactobacillus kefiranofaciens as a part of kefir granules, could be isolated and purified from the culture broth after the lactose-fermentation from whey [37,49]. To achieve this, several isolated steps can be executed like filtration, homogenization, thermal inactivation of glycolytic enzymes, polysaccharide precipitation, separation, and washing the precipitate (Figure 9).…”

Section: Small-scale Production Of Kefiranmentioning

confidence: 99%

“…In previous work, whey powder (WP) and kefir grains have been used to find the suitable conditions for the WP concentration and temperature to maximize kefiran production [37] or maximize certain desirability function formed by equally weighed kefiran as a prebiotic, and the cell concentration of LAB and yeast in a functional drink [47].…”

Section: Introductionmentioning

confidence: 99%

“…Kefiran was determined according to phenol-sulfuric acid method [48] which employed the glucose as a reference sugar similar as it had been determined elsewhere [24,37].…”

Section: Introductionmentioning

confidence: 99%

“…Due to a complexity of the consortia of microorganism presented in the kefir grain, a culture medium is supplemented either with glucose or sucrose, adjusted the brix of the medium at a constant value of 14%, as was suggested elsewhere [43,44], and it was performed in previous work [37].…”

Section: Introductionmentioning

confidence: 99%

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Small-Scale Process for the Production of Kefiran through Culture Optimization by Use of Central Composite Design from Whey and Kefir Granules

Pais-Chanfrau¹,

Acosta²,

Cóndor³

et al. 2019

Current Topics in Biochemical Engineering

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Cheese is one of the most demanded dairy products worldwide. However, during the conversion of milk to cheese, about 10 liters of milk are employed and about 9 liters of whey are generated for each 1 kg of cheese produced. The whey has traditionally been used for animal feed and as starting material for obtaining whey proteins. Furthermore, whey has the significant values of BOD and COD, becoming the most important contaminant in the dairy industry. For this reason, further growth of cheese sector is being limited by the surplus of whey as a by-product of the production of the cheeses. One of the many possibilities offered by the whey is its use as a starting material to produce many biotech products with a higher added value. The kefiran is a degradable biopolymer and is formed by galactose and glucose units, in almost similar proportions, which have been found with numerous benefits for human health. It is produced by a consortium of acid-lactic bacteria and yeasts, which coexist within the kefir granules, which are able to grow and multiply using the lactose present in the whey. The objective of the present study is to establish a small-scale process that allows the obtaining of kefiran.

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Section: Introductionmentioning

confidence: 93%

Section: Small-scale Production Of Kefiranmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Kefiran was determined according to phenol-sulfuric acid method [48] which employed the glucose as a reference sugar similar as it had been determined elsewhere [24,37].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Small-Scale Process for the Production of Kefiran through Culture Optimization by Use of Central Composite Design from Whey and Kefir Granules

Pais-Chanfrau¹,

Acosta²,

Cóndor³

et al. 2019

Current Topics in Biochemical Engineering

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Uses of the Response Surface Methodology for the Optimization of Agro-Industrial Processes

Pais-Chanfrau¹,

Núñez-Pérez²,

Espín-Valladares³

et al. 2021

Response Surface Methodology in Engineering Science

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Response surface methodology is a tool for the design of experiments, widely used today to optimize industrial processes, including agro-industrial ones. Since its appearance in the last century’s fifties, hundreds of articles, chapters of books, and books attest to this. In this work, a general overview of this tool’s general practical aspects is made. This statistical tool’s usefulness and popularity, used in the optimization of agro-industrial processes and in making them more efficient and sustainable, is described through multiple examples.

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Response Surface Methodology to Optimize a Bioprocess for Kefiran Production

Cited by 2 publications

References 0 publications

Small-Scale Process for the Production of Kefiran through Culture Optimization by Use of Central Composite Design from Whey and Kefir Granules

Small-Scale Process for the Production of Kefiran through Culture Optimization by Use of Central Composite Design from Whey and Kefir Granules

Uses of the Response Surface Methodology for the Optimization of Agro-Industrial Processes

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