Response surface optimization of mixed substrate solid state fermentation for the production of lovastatin by Monascus purpureus

Abstract

The objective of this work is to enhance the production of lovastatin using Monascus purpureus MTCC 369 in mixed substrate solid state fermentation using various solid substrates and to optimize the combination of the solid substrates by response surface methodology. Solid state fermentation was conducted in a 250 mL Erlenmeyer flask at 30°C for 14 days with initial moisture content of 40% and inoculum size of 10% active culture. Barley, long grain rice and sago starch were found to be the suitable substrates producing maximum lovastatin of 193.7 mg, 190.2 mg and 180.9 mg/g of dry solids. These substrates were further used in various combinations as designed by the central composite design for enhancing the lovastatin production using Monascus purpureus. To the best of our knowledge this is the first report on the production of lovastatin using a mixed substrate solid state fermentation using Monascus purpureus.

Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus

“…RSM is a sequential procedure with an initial objective to lead the experimenter rapidly and efficiently along a path of improvement toward the general vicinity of the optimum. Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing mevastatin production [10] , [11] , [12] , [1] , [2] , [3] . 2 5 full factorial central composite design and RSM were applied to determine the optimal for each significant variable.…”

Monacolin K production by citrinin‐free Monascus pilosusMS‐1 and fermentation process monitoring

“…However, lovastatin produced by A. terreus is normally used as a clinical drug because the strain is not generally recognized to be safe [7,8]. In the last 20 years, red fermented rice produced by M. purpureus [20][21][22][23] and M. ruber [24,25], both of which possess citrinin biosynthetic abilities [15,16]. The screening of Monascus strains with high-efficiency MK production and no citrinin was vital for MK production.…”

Enhanced production of lovastatin by Omphalotus olearius (DC.) Singer in solid state fermentation

“…11,21 In contrast, we found barley to be the best substrate, followed by wheat bran and rice husks. Also, Subhagar et al 20 reported that lovastatin production by Monascus purpureus MTCC 369 was 193.7 mg/g in SSF with barley. The particle size of the substrate is one of the most important variables in SSF because biosynthesis of lovastatin depends on the oxygen supply.…”