Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1

Abstract

Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

“…The reduction in pullulan production could be attributed to the high viscosity of broth, resulting in a limited availability of nutrients and oxygen to the culture during fermentation. Our results corroborate the findings on pullulan production from A. pullulans [44,50] and R. bacarum [4]. …”

Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1

“…The production of pullulan was carried out in shake-flasks as described earlier (Singh, Singh, & Saini, 2009). Briefly, inoculum was prepared in a medium containing (g/L) sucrose, 50.0; K 2 HPO 4, 2.0; (NH 4 ) 2 SO 4, 1.0; NaCl, 0.5; MgSO 4 Á7H 2 O, 0.05; FeSO 4, 0.01; MnSO 4, 0.01; ZnSO 4, 0.01 and adjusted to pH 7.0, at 30°C on a refrigerated rotary shaker (Innova 4335, New Brunswick Scientific, USA) under agitation (150 rpm) for 4 days.…”

Effect of carboxymethyl groups on degradation of modified pullulan by pullulanase from Klebsiella pneumoniae

“…Pullulan (Pull) is a neutral and biodegradable polysaccharide, composed of maltotriose units linked by ␣-1,6 bonds (Singh, Singh, & Saini, 2009;Teramoto & Shibata, 2006) and selectively hydrolyzed by pullulanase (EC 3.2.1.41) which can determine the chemical structure of polysaccharide and improve the production yield of many sugars (Kuroiwa, Shoda, Ichikawa, Sato & Mukataka, 2005;Mondal, Sharma, & Nath Gupta, 2003). Pullulanase is an enzyme whose hydrolyze the (1,6)␣-d-glucosidic linkages in pullulan and amylopectin.…”