The environment and scope of work of optometric personnel in ophthalmology are unknown. We aimed to investigate these topics using an online survey. Based on the results obtained, we also make policy proposals from the perspective of clinical laboratory technologists to the University-Association-Academy. The age distribution of the study subjects was as follows; 20s (40.7%), 30s (28.4%), 40s (23.5%), and 50s (7.4%). Women constituted 56.0% of the cohort, and careers were evenly distributed from <1 to ≥15 years. We found optometric personnel had high levels of work-related stress, frequent occupational diseases, and that their duties were wide in scope. More manpower is needed to reduce work stress and improve work satisfaction. Clinical laboratory technologists held more senior positions at 48% compared to others and the occupational distribution was closely related to the senior occupation (P<0.001). Clinical laboratory technologists were responsible for about 30 types of eye examinations, and the most frequently performed examination was the refraction test; anomaloscopic examinations took the most time (27 minutes). Taken together, clinical laboratory technologists perform various eye exams. Given the importance of clinical laboratory technologists in optometry, a multifaceted effort is required at the University-Association-Academy.