Response to Comment on “Circadian rhythms in the absence of the clock gene
            <i>Bmal1</i>
            ”

S, Ray; Valekunja, Utham K.; Stangherlin, Alessandra; Howell, Steven; Snijders, Ambrosius P.; Damodaran, Gopinath; Reddy, Akhilesh B.

doi:10.1126/science.abf1930

Cited by 3 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They could be the result of the mutation itself, a compensatory mechanism or false positives resulting from our analysis. The study by Ray et al, which report controversial findings regarding RNA rhythmicity in the Bmal1 -/mouse cells (NvCycle orthologous in Nematostella) is an interesting case of rhythmicity analysis to consider [34][35][36][37][38] . Our attempt to identify rhythmic genes faced challenges, particularly in the choice of statistical parameters.…”

Section: Discussionmentioning

confidence: 99%

CLOCK evolved in cnidaria to synchronize internal rhythms with diel environmental cues

Rinsky

Aguillon

Simon‐Blecher

et al. 2023

Preprint

View full text Add to dashboard Cite

The circadian clock is a crucial biological mechanism that enables organisms to anticipate the daily light/dark cycle. While the circadian clock factors underlying the molecular mechanism were characterized in a few model organisms, the evolutionary functional origin of the core clock proteins is unclear. In cnidarians, the expression of the putative core clock genes is rhythmic under light/dark cycles but is arrhythmic under constant light conditions. Thus, it is unclear how the core circadian genes regulate rhythmic behavior. Here, we studied the function of the evolutionarily conserved bHLH transcription factorClockin the sea anemoneNematostella vectensis. The CRISPR/Cas9 system was employed to generate aClockmutant (Clock-/-)N. vectensis. InClock-/-N. vectensis, rhythmic locomotor activity was observed under light/dark cycles, but the rhythmicity was abolished under constant dark or light conditions. Likewise, transcriptome profiling inClock-/-N. vectensisrevealed an array of clock-controlled genes (CCG). Additionally, we observed a co-operation betweenClockand light to shape core clock genes transcription rhythmicity. Notably, loss of CLOCK resulted in hundreds of differential expressed genes, which mainly regulate the cell cycle and the development of the nervous system. Overall, our results suggest that the CLOCK protein evolved to modulates the ancient hierarchy between light- and circadian clock-regulated physiology and behavior.

show abstract

Section: Discussionmentioning

confidence: 99%

CLOCK evolved in cnidaria to synchronize internal rhythms with diel environmental cues

Rinsky

Aguillon

Simon‐Blecher

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Recently, a groundbreaking study demonstrated the maintenance of transcriptional rhythmicity despite the absence of a functional TTFL in Bmal1-deficient mouse cells [414]. However, this finding has sparked significant controversy among chronobiologists, leading to an intense debate regarding the validity of these results [415][416][417][418]. Whether eukaryotic redox and metabolic rhythms act solely as inputs and outputs or constitute the core of eukaryotic cellular timekeeping remains to be elucidated.…”

Section: Time Cues That Facilitate Rhythmic Cooperationmentioning

confidence: 99%

Studying the Human Microbiota: Advances in Understanding the Fundamentals, Origin, and Evolution of Biological Timekeeping

Siebieszuk,

Sejbuk,

Witkowska

2023

IJMS

View full text Add to dashboard Cite

The recently observed circadian oscillations of the intestinal microbiota underscore the profound nature of the human–microbiome relationship and its importance for health. Together with the discovery of circadian clocks in non-photosynthetic gut bacteria and circadian rhythms in anucleated cells, these findings have indicated the possibility that virtually all microorganisms may possess functional biological clocks. However, they have also raised many essential questions concerning the fundamentals of biological timekeeping, its evolution, and its origin. This narrative review provides a comprehensive overview of the recent literature in molecular chronobiology, aiming to bring together the latest evidence on the structure and mechanisms driving microbial biological clocks while pointing to potential applications of this knowledge in medicine. Moreover, it discusses the latest hypotheses regarding the evolution of timing mechanisms and describes the functions of peroxiredoxins in cells and their contribution to the cellular clockwork. The diversity of biological clocks among various human-associated microorganisms and the role of transcriptional and post-translational timekeeping mechanisms are also addressed. Finally, recent evidence on metabolic oscillators and host–microbiome communication is presented.

show abstract

A time to heal: microRNA and circadian dynamics in cutaneous wound repair

et al. 2022

View full text Add to dashboard Cite

Many biological systems have evolved circadian rhythms based on the daily cycles of daylight and darkness on Earth. Such rhythms are synchronised or entrained to 24-h cycles, predominantly by light, and disruption of the normal circadian rhythms has been linked to elevation of multiple health risks. The skin serves as a protective barrier to prevent microbial infection and maintain homoeostasis of the underlying tissue and the whole organism. However, in chronic non-healing wounds such as diabetic foot ulcers (DFUs), pressure sores, venous and arterial ulcers, a variety of factors conspire to prevent wound repair. On the other hand, keloids and hypertrophic scars arise from overactive repair mechanisms that fail to cease in a timely fashion, leading to excessive production of extracellular matrix (ECM) components such as such as collagen. Recent years have seen huge increases in our understanding of the functions of microRNAs (miRNAs) in wound repair. Concomitantly, there has been growing recognition of miRNA roles in circadian processes, either as regulators or targets of clock activity or direct responders to external circadian stimuli. In addition, miRNAs are now known to function as intercellular signalling mediators through extracellular vesicles (EVs). In this review, we explore the intersection of mechanisms by which circadian and miRNA responses interact with each other in relation to wound repair in the skin, using keratinocytes, macrophages and fibroblasts as exemplars. We highlight areas for further investigation to support the development of translational insights to support circadian medicine in the context of these cells.

show abstract

Response to Comment on “Circadian rhythms in the absence of the clock gene Bmal1 ”

Cited by 3 publications

References 9 publications

CLOCK evolved in cnidaria to synchronize internal rhythms with diel environmental cues

CLOCK evolved in cnidaria to synchronize internal rhythms with diel environmental cues

Studying the Human Microbiota: Advances in Understanding the Fundamentals, Origin, and Evolution of Biological Timekeeping

A time to heal: microRNA and circadian dynamics in cutaneous wound repair

Contact Info

Product

Resources

About