Mercury is a major constituent of dental amalgam. Patients with dental amalgam restorations intake mercury vapor by inhalation, may ingest elemental mercury, or may swallow tiny fragments of amalgam [1]. X-ray absorption near-edge spectroscopy (XANES) of extracted teeth that were restored with dental amalgam for more than 20 years identified dentinal tubules as another possible portal for patients' intake of mercury [2]. K-edge subtraction (KES) imaging is a state-of-the-art capability at the Biomedical Imaging and Therapy (BMIT) beamline at the Canadian Light Source (CLS). The technique is based on subtracting two sets of micro-CT images from each other, one above and one below the K-edge energy of mercury, which is the contrast element of interest in this research. The subtraction ameliorates the visibility of mercury-containing structures and annuls the visibility of mercury-free structures in the images [3]. Motivation: The aim of this research is to apply KES imaging to investigate the presence of mercury in a tooth exhibiting metallic-like features under a non-amalgam restoration when scanned and analyzed using synchrotron radiation micro-CT imaging technologies.