Response to injury in the lens epithelium of the bullfrog (Rana catesbeiana)*1

Rothstein, H.; Weinsieder, A.; Blaiklock, Robert G.

doi:10.1016/0014-4827(64)90142-9

Cited by 19 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example a wound in the anterior lens capsule of the rabbit was shortly followed by a band of cells in DNA synthesis and mitosis moving outwards from the injury site (Harding and Srinivasan, 1961). The spatial patterns of proliferative responses also appear to vary between species; for example in the frog lens the cells responding to a similar needle injury were randomly distributed (Rothstein, Weinsieder and Blaiklock, 1964). Inter-species variations in the temporal and spatial distribution, and expression, of local regulatory factors may account for such differences.…”

Section: Table IImentioning

confidence: 96%

Smad Translocation and Growth Suppression in Lens Epithelial Cells by Endogenous TGFβ2 during Wound Repair

Saika¹,

Okada²,

Miyamoto³

et al. 2001

Experimental Eye Research

106

View full text Add to dashboard Cite

Section: Table IImentioning

confidence: 96%

Smad Translocation and Growth Suppression in Lens Epithelial Cells by Endogenous TGFβ2 during Wound Repair

Saika¹,

Okada²,

Miyamoto³

et al. 2001

Experimental Eye Research

106

View full text Add to dashboard Cite

“…In earlier works we suggested proliferation in the lens may be triggered by low-cell population density [Rothstein et al, 1964[Rothstein et al, , 1965, Upon mechanical injury, cells migrate into the wound focus and consequently leave an area in which the cell number is reduced. This condition may well act as a trigger for mitosis.…”

Section: Discussionmentioning

confidence: 99%

Hypophysectomy Inhibits Wound Hyperplasia in the Adult Frog Lens

Rothstein¹,

Buskirk²,

Reddan³

1976

Ophthalmic Res

View full text Add to dashboard Cite

The experiments reported herein prove that wound-associated hyperplasia of frog lenses is inhibited after hypophysectomy. Proliferation can be restored by replacement therapy with either frog pituitary powder or bovine somatotropin. When this is done, division figures are observed after 6 days in the environs of the wound focus and 2 days later in the germinative zone. In the absence of an injury, heightened mitotic activity typically occurs in the germinative zone and subsequently in the polar region. Wound cells in both intact and hypophysectomized frogs bind more tritiated actinomycin D than do cells in the lenses from uninjured frogs. In the latter, the amount of ³H-actinomycin bound does not seem to be affected by hypophysectomy. Wound closure occurs in experimental as well as control animals but the patterns of closure are different. We suggest that cells near the injury in the lenses of hypophysectomized animals may move from G₀ to G₁ but are stalled in the latter stage of the cycle.

show abstract

“…To test the underlying assumption more directly, mitosis was artificially stimulated by needle injury. This gives rise to increased numbers of division figures 4 days later (at 24°C) [Rothstein et al, 1964], When animals with wounded lenses were placed at 4 °C the counts were reduced from 185.4 ± 62 to 13.4 ± 3 figures. The lower number of figures, it is to be noticed, is comparable to that seen in some of the uninjured lenses a t4 nC.…”

mentioning

confidence: 99%

Mitotic Variations in the Lens Epithelium of the Frog

Rothstein

Worgul

1973

Ophthalmic Res

View full text Add to dashboard Cite

When frogs were exposed to cold temperature (4°C) the number of mitotic figures in the lens epithelium was reduced by 80–100%. However, mitotic activity does not vary directly with temperature. It is likely that other factors, such as hormones, also regulate proliferation in the germinative zone. Light-dark influences and diurnal changes have, on the other hand, not been observed to produce obvious effects. Implants of pituitary glands or lyophilized pituitaries stimulate cell division as do LH, TSH, T₃ and T₄. No steroids have yet been found effective in this regard. Somatotrophin and adrenaline are likewise ineffective. The stimulation by LH is not the result of a nonspecific immunological response, for bovine serum albumin injections exerted no detectable influence upon proliferation in the lens. When animals were transferred from 4 to 24 °C the MI increased substantially. This increase occurred on the first day of the transfer in summer frogs but took 8 days in winter frogs. Radioimmunoassay demonstrated a correlation between the number of mitosis and LH. In the transfer studies no correlation was found between serum concentrations of FSH, T₃ or T₄ and mitotic activity. A thermoendocrine hypothesis was presented to help explain the results obtained to date as well as to aid in the formulation of suitable experiments.

show abstract

Response to injury in the lens epithelium of the bullfrog (Rana catesbeiana)*1

Cited by 19 publications

References 12 publications

Smad Translocation and Growth Suppression in Lens Epithelial Cells by Endogenous TGFβ2 during Wound Repair

Smad Translocation and Growth Suppression in Lens Epithelial Cells by Endogenous TGFβ2 during Wound Repair

Hypophysectomy Inhibits Wound Hyperplasia in the Adult Frog Lens

Mitotic Variations in the Lens Epithelium of the Frog

Contact Info

Product

Resources

About