Response to Oxidative Stress in Eight Pathogenic Yeast Species of the Genus Candida

Abegg, Maxwel Adriano; Alabarse, Paulo Vinícius Gil; Casanova, Anderson; Hoscheid, Jaqueline; Salomon, Tiago Boeira; Hackenhaar, Fernanda Schäfer; Medeiros, Tássia Machado; Benfato, Mara da Silveira

doi:10.1007/s11046-010-9294-5

Cited by 32 publications

(23 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The isolates were identified and maintained as previously described 22 . Viable cells were obtained by cultivation on solid yeast extract-peptone-dextrose (YPD) medium (1% yeast extract, 2% peptone, 2% glucose, 2% agar), and isolates were then grown in liquid YPD medium in an orbital shaker at 30°C/100 rpm to late exponential growth (OD 600nm = 1.5-1.6).…”

Section: Yeast Isolates and Cultivationmentioning

confidence: 99%

“…Cell suspensions were treated with 0.5mM H 2 O 2 , washed, lysed, and centrifuged, as previously described 22 , and 5µL of each supernatant was mixed with 200µL of 0.4 mol/L acetate buffer, pH 5.8. Then, 20µL of ABTS* + in 30mM acetate buffer, pH 3.6, was added, mixed, and the absorbance measured after 5 min.…”

Section: Total Antioxidant Capacitymentioning

confidence: 99%

“…exhibit unequal oxidative stress resistances in vitro [20][21][22] , and different in vitro virulence potentials 23 , and we proposed that this may contribute to the capacity of each species to cause candidemia 22 . Taking into account these differences, total glutathione levels and the cellular TAC were assessed in 8 Candida species.…”

Section: Introductionmentioning

confidence: 97%

“…This concentration may induce 40% lethality in S. cerevisiae and is normally sublethal (95-100% viability) in the case of Candida sp. 22,39 .GSH can occur in yeasts in the reduced form (GSH), the oxidized form (GSSG), and as different mixed disulfides, for example GS-S-CoA and GS-S-Cys 13 . The H 2 O 2 (0.5 mM/1h treatment) used to induce oxidative stress was probably detoxified in part through the action of the enzyme glutathione-peroxidase (GPx) and the concomitant conversion of GSH into GSSG 29 .…”

mentioning

confidence: 99%

“…This concentration may induce 40% lethality in S. cerevisiae and is normally sublethal (95-100% viability) in the case of Candida sp. 22,39 .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Abegg

Alabarse

Schüller

et al. 2012

Rev. Soc. Bras. Med. Trop.

Self Cite

View full text Add to dashboard Cite

Candida albicans, C. glabrata, C. tropicalis, and C. parapsilosis account for approximately 95% of identifiable Candida infections. Other species, including C. krusei, C. lusitaniae, and C. guilliermondii, account for less than 5% of cases of invasive candidiasis. The most common causative agent is still C. albicans, but its incidence is declining and the frequencies of other species are increasing. Recently, Furlaneto et al. 1 noted that non-albicans Candida was the predominant species in different clinical specimens, with the exception of urine samples, in a Brazilian tertiary-care hospital. Invasive candidiasis has a mortality rate that approaches 40% 2,3 . Although most people are colonized by Candida sp., the majority never develop invasive candidiasis. Alterations in host immunity, physiological features, or normal microflora, rather than the acquisition of novel or hypervirulent factors by Candida, are suggested to degenerate the commensal-host interaction and lead to an opportunistic infection 4 .During the course of a systemic infection, Candida cells are engulfed by host phagocytes, where they are exposed to reactive oxygen species (ROS) 5 . ROS contribute to the killing of C. albicans in both cultured cells and entire organisms [6][7][8][9] . Upon incubation with macrophages, C. albicans deoxyribonucleic acid (DNA) repair genes are transcriptionally induced, suggesting that DNA damage indeed occurs in the phagosome and that genotoxic hypersensitivity stress would be disadvantageous to the pathogen 10 . Recently, it was demonstrated that a large proportion of C. albicans cell surface antigens related to acute candidemia are involved in oxidative stress 4 . In C. albicans, hyphal cells ABSTRACT Introduction: The capacity to overcome the oxidative stress imposed by phagocytes seems to be critical for Candida species to cause invasive candidiasis. Methods: To better characterize the oxidative stress response (OSR) of 8 clinically relevant Candida sp., glutathione, a vital component of the intracellular redox balance, was measured using the 5,5'-dithiobis-(2-nitrobenzoic acid (DTNB)-glutathione disulfide (GSSG) reductase reconversion method; the total antioxidant capacity (TAC) was measured using a modified method based on the decolorization of the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic) acid radical cation (ABTS* + ). Both methods were used with cellular Candida sp. extracts treated or not with hydrogen peroxide (0.5 mM). Results: Oxidative stress induced by hydrogen peroxide clearly reduced intracellular glutathione levels. This depletion was stronger in Candida albicans and the levels of glutathione in untreated cells were also higher in this species. The TAC demonstrated intra-specific variation. Conclusions: Glutathione levels did not correlate with the measured TAC values, despite this being the most important non-enzymatic intracellular antioxidant molecule. The results indicate that the isolated measurement of TAC does not give a clear picture of the ability of a given Candida sp. to resp...

show abstract

Section: Yeast Isolates and Cultivationmentioning

confidence: 99%

Section: Total Antioxidant Capacitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

mentioning

confidence: 99%

“…This concentration may induce 40% lethality in S. cerevisiae and is normally sublethal (95-100% viability) in the case of Candida sp. 22,39 .…”

mentioning

confidence: 99%

See 3 more Smart Citations

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Abegg

Alabarse

Schüller

et al. 2012

Rev. Soc. Bras. Med. Trop.

Self Cite

View full text Add to dashboard Cite

show abstract

Proteomic analysis of the inhibitory effect of oligochitosan on the fungal pathogen, Botrytis cinerea

Meng

2018

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: The fungal pathogen Botrytis cinerea infects a broad range of horticultural plants worldwide, resulting in significant economic losses. A derivative of chitosan, oligochitosan, has been reported to be an eco-friendly alternative to synthetic fungicides. RESULTS:Oligochitosan can greatly inhibit B. cinerea spore germination and induce protein carbonylation. To further investigate the molecular mechanism underlying the inhibitory effect, a comparative proteome analysis was conducted of oligochitosan-treated versus non-treated B. cinerea spores. The cellular proteins were obtained from B. cinerea spore samples and subjected to two-dimensional gel electrophoresis. In total, 21 differentially expressed proteins (DEPs) were identified. Three DEPs were up-regulated in the oligochitosan-treated versus the untreated spores, including scytalone dehydratase and a serine carboxypeptidase III precursor. By contrast, seven DEPs, including Hsp 88 and cell division cycle protein 48, were down-regulated by oligochitosan treatment. Notably, 10 DEPs, including phosphatidylserine decarboxylase proenzyme and ATP-dependent molecular chaperone HSC82, were only detected in the control spores, whereas one DEP, a non-annotated predicted protein, was only detected in the oligochitosan-treated spores. CONCLUSION: Oligochitosan may affect the spore germination of B. cinerea by impairing protein function. These findings have practical implications with respect to the use of oligochitosan for controlling fungal pathogens. A, Potential applications and antifungal activities of engineered nanomaterials against gray J Sci Food Agric 2019; 99: 2622-2628

show abstract

Current awareness on yeast

2010

Yeast

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (5 weeks journals ‐ search completed 8th. Sept. 2010)

show abstract

Response to Oxidative Stress in Eight Pathogenic Yeast Species of the Genus Candida

Cited by 32 publications

References 42 publications

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Proteomic analysis of the inhibitory effect of oligochitosan on the fungal pathogen, Botrytis cinerea

Current awareness on yeast

Contact Info

Product

Resources

About