Response to oxygen of diazotrophic Azospirillum brasilense ? Arthrobacter giacomelloi mixed batch culture

Cacciari, I.; Lippi, D.; Ippoliti, S.; Pietrosanti, T.; Pietrosanti, W.

doi:10.1007/bf00456086

Cited by 15 publications

(2 citation statements)

References 18 publications

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“…Soil samples collected from about 15 different sites that were rich in plastic wastes were used as inoculants for enrichment cultures. Portions (1 g) of starch-polyethylene blends (85% low-density polyethylene coextruded with 15% cornstarch [Polybatch Nadegl5; Schulmann]) that were repeatedly washed in sterile water were added as small strips (0.5 by 5 cm; thickness, 0.3 ,um) to 50-ml vessels containing 30 ml of sterilized B7 medium (8) supplemented with 0.2% glucose. The vessels were tightly sealed with screw caps after the addition of 1 ml of soil suspension obtained by adding 1 g of soil to 10 ml of tap water.…”

Section: Methodsmentioning

confidence: 99%

Isotactic polypropylene biodegradation by a microbial community: physicochemical characterization of metabolites produced

Cacciari

Quatrini

Zirletta

et al. 1993

Appl Environ Microbiol

172

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From a selective enrichment culture prepared with different soil samples on starch-containing polyethylene we isolated four microaerophilic microbial communities able to grow on this kind of plastic with no additional carbon source. One consortium, designated community 3S, was tested with pure isotactic polypropylene to determine whether the consortium was able to degrade this polymer. Polypropylene strips were incubated for 5 months in a mineral medium containing sodium lactate and glucose in screw-cap bottles. Dichloromethane crude extracts of the cultures revealed that the weight of extracted materials increased with incubation time, while the polypropylene sample weight decreased. The extracted materials were characterized by performing chromatographic and spectral analyses (thin-layer chromatography, liquid chromatography, gas chromatography-mass spectrometry, infrared spectrometry, nuclear magnetic resonance). Three main fractions were detected and analyzed; a mixture of hydrocarbons at different degrees of functionalization was found together with a mixture of aromatic esters, as the plasticizers usually added to polyolefinic structures.

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Section: Methodsmentioning

confidence: 99%

Isotactic polypropylene biodegradation by a microbial community: physicochemical characterization of metabolites produced

Cacciari

Quatrini

Zirletta

et al. 1993

Appl Environ Microbiol

172

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“…02 consumption was assayed at 28°C on 3 ml of the suspension by an oxygen monitor YSI mod 240/B. Nitrogenase activity was determined on 5 ml of the same suspension according to Cacciari et al (1989). Lignin degradation was determined measuring both the solid lignin, according to Moore & Johnson (1967), and soluble fractions of lignin, Fig.…”

Section: Methodsmentioning

confidence: 99%

Olive-Mill Wastewater Composting: Microbiological Aspects

Galli¹,

Pasetti²,

Fiorelli³

et al. 1997

Waste Manag Res

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Oxygen consumption, microbial growth, lignin and phenol degradation, urease, protease and nitrogenase activity were determined during the composting of an olivemill wastewaters (OMW)—wheat straw mixture. Oxygen consumption, microbial growth and urease activity were greatly enhanced during the thermophilic phase, reaching their maximum in about three weeks. Casein-hydrolysing protease showed a high initial activity which sharply decreased after 2 weeks. At the end of the thermophilic phase both phenols and lignin were reduced by about 70%. Composting enhanced diazotrophic microflora as indicated by nitrogenase activity which increased at the end of the thermophilic phase. © 1997 ISWA

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