Response to purified Cry IB protein in lab-wild type crosses of lightbrown apple moth

Suckling, D. M.; Markwick, Ngaire P.; Wigley, P. J.; Frater, Christina M.; Chilcott, C. N.; Maindonald, John

doi:10.30843/nzpp.1996.49.11426

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Leaf-roller assays were conducted using a wheatgerm assay diet (Suckling et al, 1996) and potato tuber moth assays used potato stem juice diet (Gleave et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Insecticidal activity of avidin and streptavidin against four species of pest Lepidoptera

Markwick

Christeller²,

Dochterty

et al. 2001

Entomologia Exp Applicata

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Two biotin-binding proteins, avidin and streptavidin, were found to be insecticidal to the larvae of four species of Lepidoptera -light brown apple moth, Epiphyas postvittana (Walker) (fam. Tortricidae), green-headed leaf-roller, Planotortrix octo (Dugdale) (fam. Tortricidae), brown-headed leaf-roller, Ctenopseustis obliquana (Walker) (fam. Tortricidae) and potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae). Mortality occurred in all species, but there was a wide range in susceptibility. P. operculella larvae were the most susceptible with an LC 50 of respectively, 2.3 µg ml −1 for avidin and 1.4 µg ml −1 for streptavidin after 9 days. E. postvittana larvae had an LC 50 of 43.4 µg ml −1 for avidin after 21 days, and C. obliquana larvae of 45.7 µg ml −1 for avidin after 28 days. Although significant mortality occurred in P.octo at the highest doses of avidin, there was no sufficient dosemortality response to calculate an LC 50 for this species. For all species mortality curves were steep over a close range of doses followed by a plateau where mortality did not increase significantly with dose and did not reach 100%. Mortality was significantly affected by the amount of biotin in the diet on which the parental generation had been reared. Where this was rich in biotin, significant mortality of the offspring was much lower: larval offspring of a colony of E. postvittana, reared for five generations on a biotin-free diet had an LC 50 of 5.1 µg ml −1 after 14 days compared with 76.7 µg ml −1 for larvae from a colony reared on general purpose diet. The implications for use of these proteins to confer insect resistance on transgenic plants are discussed.

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“…Leaf-roller assays were conducted using a wheatgerm assay diet (Suckling et al, 1996) and potato tuber moth assays used potato stem juice diet (Gleave et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Insecticidal activity of avidin and streptavidin against four species of pest Lepidoptera

Markwick

Christeller²,

Dochterty

et al. 2001

Entomologia Exp Applicata

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a preliminary experiment, leafroller assay diet (LRA) was prepared as described in Suckling et al. (). Six treatments, each of 50 ml diet, were prepared by adding DMSO (Sigma‐Aldrich, St Louis, MO) at 1% (0.5 ml), 2, 3, 4, and 5% (2.5 ml) to 49.5, 49, 48.5, 48, 47.5 ml warm LRA diet (45°C), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…While protein and carbohydrate digestion has been well studied and the enzymes systems characterized (e.g., Markwick et al., ), our understanding of lipid digestion remains less well understood. For example, artificial diets (Suckling et al., ) for phyllophagous insects generally need supplementation with long chain polyunsaturated fatty acids (LCPUFA). Because lipids are energy‐rich compounds they are almost certainly utilized nutritionally by lepidopteran larvae.…”

Section: Introductionmentioning

confidence: 99%

Triterpene Acids From Apple Peel Inhibit Lepidopteran Larval Midgut Lipases and Larval Growth

Christeller

McGhie

Poulton

et al. 2014

Arch Insect Biochem Physiol

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Fruit extracts from apple, kiwifruit, feijoa, boysenberry, and blueberry were screened for the presence of lipase inhibitory compounds against lepidopteran larval midgut crude extracts. From 120 extracts, six showed significant inhibition with an extract from the peel of Malus × domestica cv. "Big Red" showing highest levels of inhibition. Because this sample was the only apple peel sample in the initial screen, a survey of peels from seven apple cultivars was undertaken and showed that, despite considerable variation, all had inhibitory activity. Successive solvent fractionation and LC-MS of cv. "Big Red" apple peel extract identified triterpene acids as the most important inhibitory compounds, of which ursolic acid and oleanolic acid were the major components and oxo- and hydroxyl-triterpene acids were minor components. When ursolic acid was incorporated into artificial diet and fed to Epiphyas postvittana Walker (Tortricidae: Lepidoptera) larvae at 0.16% w/v, a significant decrease in larval weight was observed after 21 days. This concentration of ursolic acid is less than half the concentration reported in the skin of some apple cultivars.

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The effects of the broad-specificity lipase inhibitor, tetrahydrolipstatin, on the growth, development and survival of the larvae of Epiphyas postvittana (Walker) (Tortricidae, Lepidoptera)

Markwick

Poulton

McGhie

et al. 2011

Journal of Insect Physiology

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Response to purified Cry IB protein in lab-wild type crosses of lightbrown apple moth

Cited by 6 publications

References 18 publications

Insecticidal activity of avidin and streptavidin against four species of pest Lepidoptera

Insecticidal activity of avidin and streptavidin against four species of pest Lepidoptera

Triterpene Acids From Apple Peel Inhibit Lepidopteran Larval Midgut Lipases and Larval Growth

The effects of the broad-specificity lipase inhibitor, tetrahydrolipstatin, on the growth, development and survival of the larvae of Epiphyas postvittana (Walker) (Tortricidae, Lepidoptera)

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