Response to soy: T‐cell‐like reactivity in the intestine of Atlantic salmon, <i>Salmo salar</i> L.

Bakke-McKellep, Anne Marie; Frøystad, Marianne K.; Lilleeng, Einar; Daprà, Franco; Refstie, Ståle; Krogdahl, Åshild; Landsverk, Thor

doi:10.1111/j.1365-2761.2007.00769.x

Cited by 109 publications

(89 citation statements)

References 30 publications

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“…This is in agreement with past reports showing upregulation of CD4-1 transcripts in lymphoid organs of salmon and trout in response to immunogenic stimuli (16,(48)(49)(50)(51)(52)(53)(54), usually interpreted as an indicator of CD4 + T cell proliferation. Proliferation also has been reported for CD4-1 + cells isolated from ginbuna carp during allogeneic MLR as well as after Ag-specific stimulation (18).…”

Section: Discussionsupporting

confidence: 93%

Isolation and Characterization of Salmonid CD4+ T Cells

Maisey

Montero

Corripio-Miyar

et al. 2016

The Journal of Immunology

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This study reports the isolation and functional characterization of rainbow trout (Oncorhynchus mykiss) CD4-1+ T cells and the establishment of an IL-15–dependent CD4-1+ T cell line. By using Abs specific for CD4-1 and CD3ε it was possible to isolate the double-positive T cells in spleen and head kidney. The morphology and the presence of transcripts for T cell markers in the sorted CD4-1+CD3ε+ cells were studied next. Cells were found to express TCRα, TCRβ, CD152 (CTLA-4), CD154 (CD40L), T-bet, GATA-3, and STAT-1. The sorted CD4-1+ T cells also had a distinctive functional attribute of mammalian T lymphocytes, namely they could undergo Ag-specific proliferation, using OVA as a model Ag. The OVA-stimulated cells showed increased expression of several cytokines, including IFN-γ1, IL-4/13A, IL-15, IL-17D, IL-10, and TGF-β1, perhaps indicating that T cell proliferation led to differentiation into distinct effector phenotypes. Using IL-15 as a growth factor, we have selected a lymphoid cell line derived from rainbow trout head kidney cells. The morphology, cell surface expression of CD4-1, and the presence of transcripts of T cell cytokines and transcription factors indicated that this is a CD4-1+ T cell line. To our knowledge, this is the first demonstration of the presence of CD4-1+CD3ε+ T cells in salmonids. As in mammals, CD4-1+ T cells may be the master regulators of immune responses in fish, and therefore these findings and the new model T cell line developed will contribute to a greater understanding of T cell function and immune responses in teleost fish.

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Section: Discussionsupporting

confidence: 93%

Isolation and Characterization of Salmonid CD4+ T Cells

Maisey

Montero

Corripio-Miyar

et al. 2016

The Journal of Immunology

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“…The former assays were performed as described by Bakke-McKellep et al (2007) for PCNA and Sanden et al (2005) for caspase-3, using a mouse anti-PCNA (Santa Cruz Biotechnology) and cleaved caspase-3 antibody (Cell Signalling), respectively. Sections incubated with normal fish serum instead of the primary antibody or without substrate incubation were used as negative controls.…”

Section: Histological Histochemical and Immunohistochemical Proceduresmentioning

confidence: 99%

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Ortiz-Delgado¹,

Fernández²,

Sarasquete³

et al. 2014

Aquat. Biol.

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During the last decades, the finfish larviculture industry has considerably improved its rearing methods under intensive conditions, but larval quality remains one of the main problems for the proper sustainable success of this productive sector (Kou moundouros 2010, Boglione et al. 2013a). The quality of farmed fish is directly related to the quality of the fry, and depends on both organoleptic and morphological characteristics that should be as similar as possible to that of wild fish, which is considered to be the ABSTRACT: This study provides a comprehensive description of the tissue organization of nondeformed and deformed opercula and vertebrae from gilthead sea bream Sparus aurata juveniles by means of histological, histochemical and immunohistochemical approaches. Two types of opercular anomalies are described: the folding of the opercle and subopercle into the gill chamber, starting at the upper corner of the branchial cleft and extending down to its lower third; and the partial lack of the operculum (opercle, subopercle, interopercle and preopercle underdeveloped) with a regression of the loose edge extending down to its lower third. Histological observations revealed a rare type of bone remodelling process in the opercular structure, which consisted of the coalescence of contacting bone tissues (presumably from the preopercle and opercle), resulting in skeletal tissue with a trabecular aspect filled by a single-cell epithelium of cubic osteoblastic-like cells. Differences in collagen fiber thickness and its 3-dimensional arrangement between normal and deformed opercula were also found. Lordotic vertebrae were characterized by the formation of fibrous cartilage in the haemal and/or neural sides, indicating that a metaplastic shift occurred during the process of lordosis. Another major histomorphological change found in lordotic vertebrae was the complete loss of notochordal sheath integrity. Histological alterations were coupled with an imbalance of cell death and cell proliferation processes in lordotic vertebrae as well as that of bone formation/resorption, and extracellular matrix deposition activity differences which might have resulted from the remodelling process occurring in lordotic vertebrae. Altogether, these results provide an increase in our basic knowledge of bone disorders that contribute to our understanding of the mechanisms by which these skeletal anomalies appear in this fish species and which hamper its production efficiency.

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“…In salmonids, inflammation of the distal intestine (van den Ingh et al, 1991;Rumsey et al, 1994;Baeverfjord and Krogdahl, 1996) and ulcer-like lesions in the stomach (Refstie et al, 2006a)c a u s e d by antinutritional factors in the plant protein has presented problems such as reduced intestinal absorptive ability and increased disease susceptibility Bakke-McKellep et al, 2007a). Proliferation of distal intestine enterocytes of SBM-fed Atlantic salmon, Salmo salar was observed using antibodies against PCNA (Sanden et al, 2005) and changes in trypsin activity and gene expression have also been observed in both Atlantic salmon (Krogdahl et al, 2003;Lilleeng et al, 2007b) and rainbow trout, Oncorhynchus mykiss (Romarheim et al, 2006).…”

Section: Introductionmentioning

confidence: 99%