Responses of Algal Cells to Engineered Nanoparticles Measured as Algal Cell Population, Chlorophyll a, and Lipid Peroxidation: Effect of Particle Size and Type

Metzler, David M.; Erdem, Ayça; Tseng, Yao Hsiang; Huang, Ch-Hpin

doi:10.1155/2012/237284

Cited by 54 publications

(30 citation statements)

References 54 publications

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“…However, the positive effect was recorded especially for the highest concentrations and the possible explanation (nano-TiO 2 could reduce the competition for nutrients killing the bacteria) proposed by these authors are not applicable to our results, since we have performed the assays under axenic conditions, using the nutrient-rich MBL medium. According to some author's, this stimulation is particularly curious, because the adsorption of NMs to the cells surface and the subsequent block of electrolytes and metabolites transport across the membrane was suggested as the primary mechanism of nano-TiO 2 toxicity to algae (Metzler et al 2012). However, other authors have already reported the stimulatory effects of titanium both in algae and terrestrial plants, in the photosynthetic and enzymes activity, probably caused by the influence of titanium in the uptake of other essential elements by roots (Dumon and Ernst 1988).…”

Section: Discussionmentioning

confidence: 99%

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Nogueira

Lopes

Rocha-Santos

et al. 2015

Environ Sci Pollut Res

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The rapid development of nanotechnology and the increasing use of nanomaterials (NMs) raise concern about their fate and potential effects in the environment, especially for those that could be used for remediation purposes and that will be intentionally released to the environment. Despite the remarkable emerging literature addressing the biological effects of NMs to aquatic organisms, the existing information is still scarce and contradictory. Therefore, aimed at selecting NMs for the treatment of organic and inorganic effluents, we assessed the potential toxicity of NiO (100 and 10-20 nm), Fe2O3 (≈85 × 425 nm), and TiO2 (<25 nm), to a battery of aquatic organisms: Vibrio fischeri, Raphidocelis subcapitata, Lemna minor, Daphnia magna, Brachionus plicatilis, and Artemia salina. Also a mutagenic test was performed with two Salmonella typhimurium strains. Suspensions of each NM, prepared with the different test media, were characterized by dynamic light scattering (DLS) and eletrophoretic light scattering (ELS). For the assays with marine species, no toxicity was observed for all the compounds. In opposite, statistically significant effects were produced on all freshwater species, being D. magna the most sensitive organism. Based on the results of this study, the tested NMs can be classified in a decreasing order of toxicity NiO (100 nm) > NiO (10-20 nm) > TiO2 (<25 nm) > Fe2O3, allowing to infer that apparently Fe2O3 NMs seems to be the one with less risks for receiving aquatic systems.

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Section: Discussionmentioning

confidence: 99%

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Nogueira

Lopes

Rocha-Santos

et al. 2015

Environ Sci Pollut Res

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“…EC 50 values were not reported in the study. 63 Metzler et al (2012) 64 65 Figure 2 shows that algae were by far the most sensitive test organisms when compared to bacteria and protozoa. Analogously, in their review on ecotoxicity of synthetic NPs (including ZnO, CuO and TiO 2 ) Kahru and Dubrouguier (2010) 62 showed that algae and crustaceans were the most sensitive and thus probably the 'weakest link' in aquatic exposure to NPs.…”

Section: Toxicity To Alga Pseudokirchneriella Subcapitatamentioning

confidence: 99%

Toxicity of 12 metal-based nanoparticles to algae, bacteria and protozoa

Aruoja

Pokhrel

Sihtmäe

et al. 2015

Environ. Sci.: Nano

161

114

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The use of metal based nanoparticles (NPs) is increasing which leads to their release in water bodies via various waste streams and warrants risk assessment. Consistent biological effect data on NPs for environmentally relevant test species that are accompanied by thorough characterization of NPs are scarce but indispensable for understanding possible risks of NPs. We composed and tested a library of 12 metal-based nanoparticles () using alga Pseudokirchneriella subcapitata, three bacterial species (Vibrio fischeri, Escherichia coli, Staphylococcus aureus) and protozoan Tetrahymena thermophila. The NPs were characterized for physico-chemical properties, solubility and abiotic reactive oxygen species (ROS) production. Also, respective soluble salts were analysed for toxic effects. The algal growth inhibition assay proved the most sensitive and yielded EC 50 values for 10 NPs ranging from 0.1 to 58 mg/l. Algal toxicity correlated with abiotic ROS production of NPs and the majority of NPs formed agglomerates that entrapped algal cells. Despite of different sensitivity, there was a common trend in the toxicity of NPs across different species and test formats: CuO and ZnO had highest toxicity (EC 50 values below 1 mg/l) to all organism groups except protozoa. The high toxicity was mostly due to shedding of toxic concentrations of Zn and Cu ions; for most of the test species Al 2 O 3 , SiO 2 , WO 3 and Sb 2 O 3 were not toxic below 100 mg/l and MgO showed no adverse effect below 100 mg/l to any test species in any test setting. Nano impactThe lack of good quality nanotoxicity data for environmentally relevant test species accompanied by physico-chemical characterization of nanoparticles (NPs) severely hampers risk assessment. This study provides biological response data of a thoroughly characterized library of metal-based NPs using algal, bacterial and protozoan tests. It includes correlations between NP properties and toxicity, as well as results obtained in deionized water, thus eliminating medium-specific effects. Similar trends across species are shown, although algae proved the most sensitive. EC 50 values of 10 NPs ranged from 0.1 to 58 mg/l. The values may be used for toxicity modelling or directly for risk assessment, as the 72 h algal growth inhibition data are mandatory for the registration of chemicals in the European Union.EC 50 -half effective concentration; MBC -Minimal Biocidal Concentration, i.e., the lowest tested nominal concentration of NPs which completely inhibited the formation of visible colonies after sub-culturing on toxicant-free agarised growth medium. Prior subculturing bacteria were incubated with NPs for 24 h at 25 °C in deionised water.

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“…Because of being primary agents of organic matter transformation, regeneration of nutrients, and as a food source for higher trophic levels, microorganisms are a great concern for toxicity studies (Foster et al 2011;Pagnout et al 2012). Several studies and reviews have been reported which aimed to determine the toxicity of nano-TiO 2 on different organisms such as microorganisms (Coleman et al 2005;Huang et al 2000;Simon-Deckers et al 2009;von Moos and Slaveykova 2014;Wei et al 1994) and algae (Clément et al 2013;Metzler et al 2011Metzler et al , 2012. However, relatively few studies have dealt with the direct effects of TiO 2 NPs on bacteria.…”

Section: Introductionmentioning

confidence: 99%

The short-term toxic effects of TiO2 nanoparticles toward bacteria through viability, cellular respiration, and lipid peroxidation

Erdem

Metzler

Daniel

et al. 2015

Environ Sci Pollut Res

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To better understand the potential impacts of metal oxide nanoparticles (NPs) on Gram(+) Bacillus subtilis and Gram(-) Escherichia coli (K12) bacteria, eight different nanosized titanium dioxide (TiO2) suspensions with five different concentrations were used. Water quality parameters (pH, temperature, and ionic strength), light sources, and light intensities were also changed to achieve different environmental conditions. The photosensitive TiO2 NPs were found to be harmful to varying degrees under ambient conditions, with antibacterial activity increasing with primary particle sizes from 16 to 20 nm. The presence of light was a significant factor under most conditions tested, presumably due to its role in promoting generation of reactive oxygen species (ROS). However, bacterial growth inhibition was also observed under dark conditions and different water quality parameters, indicating that undetermined mechanisms additional to photocatalytic ROS production were responsible for toxicity. The results also indicated that nano-TiO2 particles in the absence and the presence of photoactivation induced lipid peroxidation and cellular respiration disruption.

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Responses of Algal Cells to Engineered Nanoparticles Measured as Algal Cell Population, Chlorophyll a, and Lipid Peroxidation: Effect of Particle Size and Type

Cited by 54 publications

References 54 publications

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Assessing the ecotoxicity of metal nano-oxides with potential for wastewater treatment

Toxicity of 12 metal-based nanoparticles to algae, bacteria and protozoa

The short-term toxic effects of TiO2 nanoparticles toward bacteria through viability, cellular respiration, and lipid peroxidation

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