Responses of antibiotics, antibiotic resistance genes, and mobile genetic elements in sewage sludge to thermal hydrolysis pre-treatment and various anaerobic digestion conditions

Sun, Chenxiang; Li, Wei; Chen, Zhan; Qin, Wentao; Wen, Xianghua

doi:10.1016/j.envint.2019.105156

Cited by 96 publications

(26 citation statements)

References 68 publications

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“…TH reduced the abundance of all genes, but some of these genes rebounded during subsequent MAD. The effectiveness of TH at removing ARGs and antibiotics at pilot scale has previously been reported [39]. Interestingly, an ARG rebound after TH was also observed in that study, which, showed that subsequent thermophilic AD reduced this increase.…”

Section: Fate Of Args and Mges During Th-mad And Madsupporting

confidence: 77%

Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

et al. 2020

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Antibiotic resistant bacteria (ARB) and their genes (ARGs) have become recognised as significant emerging environmental pollutants. ARB and ARGs in sewage sludge can be transmitted back to humans via the food chain when sludge is recycled to agricultural land, making sludge treatment key to control the release of ARB and ARGs to the environment. This study investigated the fate of antibiotic resistant Escherichia coli and a large set of antibiotic resistance genes (ARGs) during full scale anaerobic digestion (AD) of sewage sludge at two U.K. wastewater treatment plants and evaluated the impact of thermal hydrolysis (TH) pre-treatment on their abundance and diversity. Absolute abundance of 13 ARGs and the Class I integron gene intI1 was calculated using single gene quantitative (q) PCR. High through-put qPCR analysis was also used to determine the relative abundance of 370 ARGs and mobile genetic elements (MGEs). Results revealed that TH reduced the absolute abundance of all ARGs tested and intI1 by 10–12,000 fold. After subsequent AD, a rebound effect was seen in many ARGs. The fate of ARGs during AD without pre-treatment was variable. Relative abundance of most ARGs and MGEs decreased or fluctuated, with the exception of macrolide resistance genes, which were enriched at both plants, and tetracyline and glycopeptide resistance genes which were enriched in the plant employing TH. Diversity of ARGs and MGEs decreased in both plants during sludge treatment. Principal coordinates analysis revealed that ARGs are clearly distinguished according to treatment step, whereas MGEs in digested sludge cluster according to site. This study provides a comprehensive within-digestor analysis of the fate of ARGs, MGEs and antibiotic resistant E. coli and highlights the effectiveness of AD, particularly when TH is used as a pre-treatment, at reducing the abundance of most ARGs and MGEs in sludgeand preventing their release into the environment.

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Section: Fate Of Args and Mges During Th-mad And Madsupporting

confidence: 77%

Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

et al. 2020

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“…They reported that hydrothermal pretreatments at 200 and 180°C for 20 min were sufficient for the complete decomposition of cephalosporin C in low and high saccharide content of antibiotic fermentation residues, respectively. Sun et al [30] evaluated the hydrothermal pretreatment of sewage sludge at 160°C for 30 min and found decline in tetracycline concentration from 68.6 ng/g to below detection limit (12 ng/g). OTC was found to be easily hydrolyzed both in water and soil when the temperature is higher than 45°C [31,32].…”

Section: Hydrothermal Pretreatment Conditionsmentioning

confidence: 99%

Hydrothermal pretreatment of oxytetracycline fermentation residue: Removal of oxytetracycline and increasing the potential for anaerobic digestion

Awad

Tian

Zhang

et al. 2020

Environmental Engineering Research

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The presence of high level of antibiotics in the antibiotic fermentation residue is one of the main reasons that prevent their direct disposal or further use as a resource. In this study, the feasibility of using the hydrothermal pretreatment for removing oxytetracycline (OTC) from its fermentation residue and enhancing anaerobic digestion was evaluated under different temperatures i.e. 110, 130, 150 and 170°C. The results showed that the removal rate of OTC increased as a function of temperature, and hydrothermal treatment at 130°C for 5 min was found sufficient to reduce the concentration of OTC from 3.9 mg/g to less than the detection limit (i.e., 0.25 ng/g). Biochemical methane potential tests showed that the cumulative methane production over 23 d was 73.7, 215.9, 656.8, and 439.0NmL CH4/gVS for the raw residue and the residue treated at 130, 150, and 170°C for 5 min, respectively. At the same time, the abundances of tetracycline resistance genes were reduced by hydrothermal treatments followed by anaerobic digestion. Conclusively, it is suggested that hydrothermal treatment at 150°C for 5 min was found beneficial for OTC fermentation residues ensuring the removal of OTC and further use of the residue for anaerobic digestion.

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“…Biological treatment of wastewater includes aerobic treatment, anaerobic treatment, and combination of aerobic and anaerobic treatment (Sun, Li, Chen, Qin, & Wen, 2019; Zhu et al, 2019). Biological treatment plays an important role in water pollution control (Kumari, Alam, & Siddiqi, 2019; Kumari, Tyagi, & Jagadevan, 2019).…”

Section: Biological Treatment Methodsmentioning

confidence: 99%

Emerging pollutants—Part II: Treatment

Liu

Zhang

Chang

2020

Water Environment Research

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Emerging pollutants (EPs) refer to a class of pollutants, which are emerging in the environment or recently attracted attention. EPs mainly include pharmaceutical and personal care products (PPCPs), endocrine-disrupting chemicals (EDCs), and antibiotic resistance genes (ARGs). EPs have potential threats to human health and ecological environment. In recent years, the continuous detections of EPs in surface and ground water have brought huge challenges to water treatment and also made the treatment of EPs become an international research hotspot. This paper summarizes some research results on EPs treatment published in 2019. This paper may be helpful to understand the current situations and development trends of EP treatment technologies.

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Responses of antibiotics, antibiotic resistance genes, and mobile genetic elements in sewage sludge to thermal hydrolysis pre-treatment and various anaerobic digestion conditions

Cited by 96 publications

References 68 publications

Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

Hydrothermal pretreatment of oxytetracycline fermentation residue: Removal of oxytetracycline and increasing the potential for anaerobic digestion

Emerging pollutants—Part II: Treatment

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