Responses of antioxidant enzymes and key resistant substances in perennial ryegrass (Lolium perenne L.) to cadmium and arsenic stresses

This study aimed to investigate the physiological and cellular mechanisms of Sesuvium portulacastrum under heavy metal stress to evaluate possible tolerance and adaptation mechanisms in a metal-polluted environment. The physiological and cellular ultrastructural responses of S. portulacastrum were studied hydroponically under exposure to a range of cadmium (Cd) concentrations (50 µM to 600 µM) for 28 days. The activity of antioxidant enzymes like catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD), changes in chlorophyll, and cellular ultrastructural content were examined. There was no significant difference in chlorophyll content in the leaf under the stress of 300 μM, but 400 μM and 600 μM Cd stress showed significantly decreased chlorophyll content. The SOD activity indicates an increase under the Cd stress of 100 μM for leaves, 300 μM for stems, and 50 μM for roots; after that, the SOD activity gradually decreased with increasing Cd concentrations. But POD activity was considerably increased with increasing Cd stress. CAT activity showed a gradual increase in concentrations until 300 μM of Cd stress and then decreased sharply in roots, stems, and leaf tissues. Cd stress had a considerable impact on the structure of the roots, stems, and leaves cells, such as distorted and thinner cell walls and the deformation of chloroplasts, mitochondria, and other organelles. Therefore, the increased number of nucleolus in the cell nucleus suggests that cells may be able to maintain their protein synthesis in a stressful environment. This study concludes that SOD is the dominant antioxidant enzyme activity during low Cd toxicity (<100 μM), while POD is the dominant enzyme activity during higher Cd toxicity (>100 μM).

Section: Introductionmentioning

confidence: 99%

Physiological and Cellular Ultrastructural Responses of Sesuvium portulacastrum under Cd Stress Grown Hydroponically

Uddin,

Chen,

et al. 2023

“…The apple leaves were isolated from three independent apple plants treated with pathogen C. gloeosporioides for 3 d. SOD and POD activity was measured according to previously reported methods [ 68 ]. The experiments were conducted three times.…”

Section: Methodsmentioning

confidence: 99%

MIR390 Is Involved in Regulating Anthracnose Resistance in Apple

Shi

Jiang

Zhang

et al. 2022

As an important cash crop in China, apple has a good flavor and is rich in nutrients. Fungal attacks have become a major obstacle in apple cultivation. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens in apple. Thus, discovering resistance genes in response to C. gloeosporioides may aid in designing safer control strategies and facilitate the development of apple resistance breeding. A previous study reported that ‘Hanfu’ autotetraploid apple displayed higher C. gloeosporioides resistance than ‘Hanfu’ apple, and the expression level of mdm-MIR390b was significantly upregulated in autotetraploid plants compared to that in ‘Hanfu’ plants, as demonstrated by digital gene expression (DGE) analysis. It is still unclear, however, whether mdm-MIR390b regulates apple anthracnose resistance. Apple MIR390b was transformed into apple ‘GL-3′ plants to identify the functions of mdm-MIR390b in anthracnose resistance. C. gloeosporioides treatment analysis indicated that the overexpression of mdm-MIR390b reduced fungal damage to apple leaves and fruit. Physiology analysis showed that mdm-MIR390b increased C. gloeosporioides resistance by improving superoxide dismutase (SOD) and peroxidase (POD) activity to alleviate the damage caused by O2− and H2O2. Our results demonstrate that mdm-MIR390b can improve apple plants’ anthracnose resistance.

“…Following centrifugation at 4 • C and 12,000 rpm for 10 min, the supernatant was collected and kept on ice for measurement. The SOD and POD activities were determined at 450 nm and 470 nm, respectively, as described in the protocol [57].…”

Section: Determination Of Antioxidant Enzyme Activitymentioning

confidence: 99%

Physiological and Transcriptome Analyses Reveal the Protective Effect of Exogenous Trehalose in Response to Heat Stress in Tea Plant (Camellia sinensis)

Zheng,

Liu,

Zhou

et al. 2024

It is well known that application of exogenous trehalose can enhance the heat resistance of plants. To investigate the underlying molecular mechanisms by which exogenous trehalose induces heat resistance in C. sinensis, a combination of physiological and transcriptome analyses was conducted. The findings revealed a significant increase in the activity of superoxide dismutase (SOD) and peroxidase (POD) upon treatment with 5.0 mM trehalose at different time points. Moreover, the contents of proline (PRO), endogenous trehalose, and soluble sugar exhibited a significant increase, while malondialdehyde (MDA) content decreased following treatment with 5.0 mM trehalose under 24 h high-temperature stress (38 °C/29 °C, 12 h/12 h). RNA-seq analysis demonstrated that the differentially expressed genes (DEGs) were significantly enriched in the MAPK pathway, plant hormone signal transduction, phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, flavonoid biosynthesis, and the galactose metabolism pathway. The capability to scavenge free radicals was enhanced, and the expression of a heat shock factor gene (HSFB2B) and two heat shock protein genes (HSP18.1 and HSP26.5) were upregulated in the tea plant. Consequently, it was concluded that exogenous trehalose contributes to alleviating heat stress in C. sinensis. Furthermore, it regulates the expression of genes involved in diverse pathways crucial for C. sinensis under heat-stress conditions. These findings provide novel insights into the molecular mechanisms underlying the alleviation of heat stress in C. sinensis with trehalose.