Responses of bacterial and bacteriophage communities to long-term exposure to antimicrobial agents in wastewater treatment systems

Du, Bingbing; Wang, Qiang; Yang, Qi; Wang, Ruifei; Yuan, Wei; Yan, Luyu

doi:10.1016/j.jhazmat.2021.125486

Cited by 14 publications

(5 citation statements)

References 63 publications

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“…In the current study, Runella (5%), Dechloromonas (5%), Nitrospira (4%), Haliangium (4%), and Haliscomenobacter (3%) were identified as the most prevalent genera within the bacterial community. The importance of these five predominant genera, despite the existence of antimicrobial agents, points to possible resistance and their ability to develop in a community in which antibiotic-sensitive ones could have decreased, and this observation aligns with previous studies that have revealed similar findings [10,20]. Another genus found and notable in the BP reactor is Zoogloea (2%), and this group of bacteria is typically found in activated sludge and involved in the degradation of organic and inorganic compounds in wastewater [29].…”

Section: Interactions Between Bacteriophage and Indigenous Prokaryoti...supporting

confidence: 90%

“…Illumina sequencing provided comprehensive data about the microbial community structure, and indicated that the exposure to the combination of pyophage cocktail and antibiotics resulted in substantial alterations in the microbial community structure of the reactor sludge (Figure 2). At the phylum level, Proteobacteria accounted for 62% of the bacterial population, showing an increase due to the tolerance, while the second most abundant phylum Bacteroidetes has a ratio of 23% in the BP reactor with antimicrobials, similar to the previous study [20]. On the other hand, Firmicutes is known as the third predominant phylum in WWTPs [21].…”

Section: Interactions Between Bacteriophage and Indigenous Prokaryoti...supporting

confidence: 84%

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Bacteriophage and Their Potential Use in Bioaugmentation of Biological Wastewater Treatment Processes

Reisoglu,

Aydin

2023

Sustainability

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In wastewater treatment plants, the stability of the related microbiota is pivotal for a steady and appropriate operation in biological wastewater treatment (BWT). The interactions between phages, which are high in amounts and diversity, and their particular hosts are significant due to their specificity in this microbial community. As drivers of diversity, phages are capable of changing the microbial structure by affecting host cells. This study aimed to enhance the stability of the functional microbiota, a primary concern of BWT, by evaluating the influence of bioaugmentation with bacteriophage cocktails. The sequencing data revealed significant alterations in the entire microbiome, including microeukaryotes, induced by the bacteriophages. These alterations led to a reduction in opportunistic microorganisms while preserving the beneficial ones. The genus Proteobacteria was found unaffected by three antibiotics in the bioreactor due to its antibiotic-resistant members, leading to its further growth, while the antibiotic-sensitive genus decreased in quantity. Furthermore, the phages notably influenced the metabolic pathway such as nitrogen, carbohydrate, and amino acid metabolisms by eliminating opportunistic microbes and providing improved growth conditions to bacterial species that are essential for effective reactor performance and wastewater treatment.

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Section: Interactions Between Bacteriophage and Indigenous Prokaryoti...supporting

confidence: 90%

Section: Interactions Between Bacteriophage and Indigenous Prokaryoti...supporting

confidence: 84%

Bacteriophage and Their Potential Use in Bioaugmentation of Biological Wastewater Treatment Processes

Reisoglu,

Aydin

2023

Sustainability

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“…They play a key role both in microbial ecology and microbial evolution, having the ability to alter the population dynamics within microbial communities and modify bacterial genomes through horizontal gene transfer [ 5 , 6 , 7 ]. In biological WWTPs, phage concentrations are estimated to be approximately 10 8 –10 9 particles per milliliter [ 8 , 9 , 10 , 11 ], which is higher than many other ecosystems studied to date [ 6 , 10 ]. Studies have shown that some of phage agents can control the growth of E. coli in biological WWTPs [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Expansion of Kuravirus-like Phage Sequences within the Past Decade, including Escherichia Phage YF01 from Japan, Prompt the Creation of Three New Genera

Batinovic

Fujii

Nittami

2023

Viruses

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Bacteriophages, viruses that infect bacteria, are currently receiving significant attention amid an ever-growing global antibiotic resistance crisis. In tandem, a surge in the availability and affordability of next-generation and third-generation sequencing technologies has driven the deposition of a wealth of phage sequence data. Here, we have isolated a novel Escherichia phage, YF01, from a municipal wastewater treatment plant in Yokohama, Japan. We demonstrate that the YF01 phage shares a high similarity to a collection of thirty-five Escherichia and Shigella phages found in public databases, six of which have been previously classified into the Kuravirus genus by the International Committee on Taxonomy of Viruses (ICTV). Using modern phylogenetic approaches, we demonstrate that an expansion and reshaping of the current six-membered Kuravirus genus is required to accommodate all thirty-six member phages. Ultimately, we propose the creation of three additional genera, Vellorevirus, Jinjuvirus, and Yesanvirus, which will allow a more organized approach to the addition of future Kuravirus-like phages.

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“…Transmission electron microscopy (TEM) analysis showed that Acinetobacter virus Abgy202141 had an icosahedron head (~59 nm) with a short tail (~23 nm) ( Figure 1B ). The underground sewage from residential areas contains abundant bacteria and phages, and it can serve as a preferred resource for phage isolations which survive on drug-resistant bacteria (Du et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation, characterization and therapeutic evaluation of a new Acinetobacter virus Abgy202141 lysing Acinetobacter baumannii

Tian,

Liu,

Zhou

et al. 2024

Front. Microbiol.

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Acinetobacter baumannii is an opportunistic pathogen that easily resists currently available antibiotics. Phages are considered alternative therapeutic agents to conventional antibiotics for the treatment of multidrug-resistant bacteria. We isolated an Acinetobacter virus Abgy202141 from underground sewage in a residential area of Guiyang City in China. Transmission electron microscopy (TEM) analysis showed that Acinetobacter virus Abgy202141 has an icosahedral head attached to a tail. This phage infects A. baumannii strain GY-4, and was found to have a short latent period of 5 min and with a burst size of 189 particles per infected host cell. Additionally, Acinetobacter virus Abgy202141 remained stable at different concentrations of chloroform and varying pH levels and temperatures. Based on SDS-PAGE analysis, it contained 14 proteins with molecular weights ranging from 12 to 125 kDa. The double-strand (ds) DNA genome of Acinetobacter virus Abgy202141 consisted of 41,242 bp with a GC content of 39.4%. It contained 50 open reading frames (ORFs), of which 29 ORFs had identified functions, but no virulence-related genes, antibiotic-resistance genes, or tRNAs were found. Phylogenetic analysis indicated that Acinetobacter virus Abgy202141 was a new phage in the Friunavirus genus. Acinetobacter virus Abgy202141 also showed the ability to prevent A. baumannii infections in the Galleria mellonella in vivo model.

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Responses of bacterial and bacteriophage communities to long-term exposure to antimicrobial agents in wastewater treatment systems

Cited by 14 publications

References 63 publications

Bacteriophage and Their Potential Use in Bioaugmentation of Biological Wastewater Treatment Processes

Bacteriophage and Their Potential Use in Bioaugmentation of Biological Wastewater Treatment Processes

Expansion of Kuravirus-like Phage Sequences within the Past Decade, including Escherichia Phage YF01 from Japan, Prompt the Creation of Three New Genera

Isolation, characterization and therapeutic evaluation of a new Acinetobacter virus Abgy202141 lysing Acinetobacter baumannii

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