Responses of CYP450 in the mussel Perna viridis after short-term exposure to the DSP toxins-producing dinoflagellate Prorocentrum lima

Wei, Xiaomeng; Lu, Mi-Yu; Duan, Guo-Fang; Li, Hongye; Liu, Jie‐Sheng; Yang, Wei‐Dong

doi:10.1016/j.ecoenv.2019.03.073

Cited by 21 publications

(18 citation statements)

References 52 publications

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“…Recently, based on the finding that the content of DSTs was significantly decreased in mussels when the activity of CYP3A4 was inhibited by ketoconazole, Wei et al proposed that CYP3A4 might play an important role in DST metabolism in bivalves. So, we assayed the changes in CYP3A4 and CYP3A1 transcripts [24]. We found that the expression of CYP3A4 in the digestive gland of the P. lima-exposed mussel was significantly up-regulated at 48 h compared with the control counterparts.…”

Section: Discussionmentioning

confidence: 97%

Cinnamaldehyde Could Reduce the Accumulation of Diarrhetic Shellfish Toxins in the Digestive Gland of the Mussel Perna viridis under Laboratory Conditions

et al. 2021

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Diarrhetic shellfish toxins (DSTs), some of the most important phycotoxins, are distributed almost all over the world, posing a great threat to human health through the food chain. Therefore, it is of great significance to find effective methods to reduce toxin accumulation in shellfish. In this paper, we observed the effects of four phytochemicals including cinnamaldehyde (CA), quercetin, oridonin and allicin on the accumulation of DSTs in the digestive gland of Perna viridis after exposure to the DSTs-producing Prorocentrum lima. We found that, among the four phytochemicals, CA could effectively decrease the accumulation of DSTs (okadaic acid-eq) in the digestive gland of P. viridis. Further evidence demonstrated that CA could reduce the histological alterations of the digestive gland of a mussel caused by DSTs. RT-qPCR showed that CA could suppress the CYP3A4 induction by DSTs, suggesting that the DSTs’ decrease induced by CA might be related to the inhibition of CYP3A4 transcription induction. However, further studies on the underlying mechanism, optimal treatment time, ecological safety and cost should be addressed before cinnamaldehyde is used to decrease the accumulation of DSTs in field.

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Section: Discussionmentioning

confidence: 97%

Cinnamaldehyde Could Reduce the Accumulation of Diarrhetic Shellfish Toxins in the Digestive Gland of the Mussel Perna viridis under Laboratory Conditions

et al. 2021

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“…Many studies suggest that these mollusks have developed cellular mechanisms of protection to overcome the harmful effects of phycotoxins. The involvement of the first line defense antioxidants (such as SOD, CAT and GPx) [ 72 ], phase-I and -II drug metabolizing enzymes cytochrome P450 (CYP450) and glutathione s-transferase (GST) [ 73 , 74 ] and ATP-binding cassette (ABC) transporters (e.g., P-glycoprotein) [ 75 , 76 ] have been related to P. lima and associated toxins resistance in bivalves.…”

Section: Ecotoxicological Effects Of Dstsmentioning

confidence: 99%

“…Moreover, these genes and proteins demonstrated to respond to the presence of DST-producing P. lima and OA therefore constitute important molecular markers of exposure to this toxin. For instance, CYP2D14-like was found up-regulated in P. viridis digestive gland at 2 and 6 h and significantly down-regulated in the gills after 6 h of exposure [ 73 ]. On the other hand, CYP3A4 expression increased at 2 h in the gills but also at 12 h in both gills and digestive glands, while CYP3L3 and CYP2C8 were down-regulated at 12 h and upregulated at 6 h, respectively [ 73 ].…”

Section: Biomarkers Of Exposure To Dstsmentioning

confidence: 99%

“…For instance, CYP2D14-like was found up-regulated in P. viridis digestive gland at 2 and 6 h and significantly down-regulated in the gills after 6 h of exposure [ 73 ]. On the other hand, CYP3A4 expression increased at 2 h in the gills but also at 12 h in both gills and digestive glands, while CYP3L3 and CYP2C8 were down-regulated at 12 h and upregulated at 6 h, respectively [ 73 ]. In scallop A. irradians , the exposure to OA induced the expression of genes encoding several ABC transporters ( ABCB10 , ABCC5 and ABCC1 ) [ 140 ] and in contaminated P. viridis P-GP gene was also up-regulated [ 75 ].…”

Section: Biomarkers Of Exposure To Dstsmentioning

confidence: 99%

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OMICs Approaches in Diarrhetic Shellfish Toxins Research

Campos

Freitas

Almeida

et al. 2020

Toxins

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Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

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“…Some studies indicate that OA produces the most dramatic effects at low concentrations after short exposure duration, whereas high concentrations and longer exposures initiate resistance mechanisms (Prego-Faraldo et al, 2015). Cytochrome P450, Glutathione S-transferases, ABC transporters (P-glycoprotein) and antioxidant metabolism enzymes (such as superoxide dismutase and catalase) have been associated to DSTs resistance in bivalves (Huang et al, 2014;Zou et al, 2015;Prego-Faraldo et al, 2017;Wei et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Molecular Responses of Mussel Mytilus galloprovincialis Associated to Accumulation and Depuration of Marine Biotoxins Okadaic Acid and Dinophysistoxin-1 Revealed by Shotgun Proteomics

et al. 2020

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The molecular pathways behind the toxicity of diarrheic shellfish toxins (DSTs) in bivalves have been scarcely studied. Thus, a shotgun proteomics approach was applied in this work to understand bivalves’ molecular responses to the dinoflagellate Prorocentrum lima (1.0 × 106 cells/L). Protein expression along with toxins levels were analyzed in the gills and digestive gland of the mussel Mytilus galloprovincialis during and after exposure to this toxic strain. Results revealed an accumulation of OA and DTX1 only in the digestive gland with maximum amounts attained at the end of uptake phase (day 5; 2819.2 ± 522.2 μg OA/kg and 1107.1 ± 267.9 μg DTX1/kg). At the end of the depuration phase (day 20), 16% and 47% of total OA and DTX1 concentrations remained in the digestive gland tissues, respectively. The shotgun proteomic analyses yielded 3051 proteins in both organs. A total of 56 and 54 differentially expressed proteins (DEPs) were revealed in the digestive gland and gills, respectively. Both organs presented the same response dynamics along the experiment, although with tissue-specific features. The early response (3 days uptake) was characterized by a high number of DEPs, being more marked in gills, in relation to the latter time points (5 days uptake and depuration). Functional enrichment analysis revealed the up-regulation of carboxylic (GO:0046943) and organic acid transmembrane transporter activity (GO:0005342) pathways after 3 days uptake for digestive gland. Matching to these pathways are a group of proteins related to transmembrane transport and response to toxic substances and xenobiotics, namely P-glycoprotein (ABCB11), Sodium-dependent proline transporter (SLC6A7), and Sideroflexin-1 (SFXN1). According to Clusters of Orthologous Groups (GOs) categories, most of the DEPs found for digestive gland in all time-points were related with “cellular processes and signaling” and involving signal transduction mechanisms, cytoskeleton and post-translational modification, protein turnover, chaperone functions. In gills, the early uptake phase was marked by a balance between DEPs related with “cellular processes and signaling” and “metabolism.” Depuration is clearly marked by processes related with “metabolism,” mainly involving secondary metabolites biosynthesis, transport, and catabolism. Proteomic data are available via ProteomeXchange with identifier PXD022293.

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Responses of CYP450 in the mussel Perna viridis after short-term exposure to the DSP toxins-producing dinoflagellate Prorocentrum lima

Cited by 21 publications

References 52 publications

Cinnamaldehyde Could Reduce the Accumulation of Diarrhetic Shellfish Toxins in the Digestive Gland of the Mussel Perna viridis under Laboratory Conditions

Cinnamaldehyde Could Reduce the Accumulation of Diarrhetic Shellfish Toxins in the Digestive Gland of the Mussel Perna viridis under Laboratory Conditions

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Molecular Responses of Mussel Mytilus galloprovincialis Associated to Accumulation and Depuration of Marine Biotoxins Okadaic Acid and Dinophysistoxin-1 Revealed by Shotgun Proteomics

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