Mammalian daily activity is shaped by a combination of intrinsic and extrinsic factors. Age influences activity rhythms due to energy requirements, while physiological and reproductive traits cause differences between genders. In ecosystems with marked climatic seasonality, such as the seasonally dry tropical forest (SDTF), activity patterns adapt to extrinsic factors like resource availability and environmental stress. This study investigates how intrinsic factors, specifically age and sex, influence the white-tailed deer's (Odocoileus virginianus) daily activities, and how these vary between dry and rainy seasons. Between 2015 and 2018, we conducted a camera trapping study to monitor the daily activity of a population of white-tailed deer in the Arenillas Ecological Reserve, southwestern Ecuador. We estimated individual daily activity based on four parameters: total and diurnal relative abundance index (RAI), activity directionality, and activity overlap between groups. We used generalized linear models to evaluate the changes in RAI based on age-classes and sex of the individuals. The Watson test was employed to assess differences in directional patterns during activity hours, while the Wald test was utilized to evaluate significant variations in activity overlap. The same analyses were also performed to assess changes in daily activity between the dry and rainy seasons. The daily activity patterns of white-tailed deer varied by age and sex. Fawns were predominantly diurnal, whereas adults displayed continuous activity throughout the 24 hours of the day, with males being more active during the night than females. Females did not show significant differences in the activity pattern compared to fawns and juveniles. The daily activity pattern of white-tailed deer varied between seasons. Overall, there was an increase in daily activity during the dry season, but significant only for males. Females were the only group that showing seasonal variation in activity directionality, with more morning activity during the dry season. The daily activity patterns of white-tailed deer in the dry forest exhibit slight differences compared to those observed in other ecosystems, with extended daily activity periods. During the rainy season, reproductive and post-reproductive behaviors, rather than resource abundance, predominantly shaped the white-tailed deer's daily activity patterns. In contrast, the dry season presented a notable rise in overall activity and daily activity, accompanied by partition between groups. This partition likely stems from diminished resource accessibility and increased intraspecific competition. Given the slight differences in the white-tailed deer's daily activity from those noted in other regions, these insights are crucial for formulating management and conservation strategies tailored to specific environmental conditions.