Responses of Freshwater Planarian Girardia tigrina to Fipronil-Based Insecticide: Survival, Behavioral and Physiological Endpoints

Reis, Eloisa Borges dos; Farnese, Fernanda dos Santos; Oliveira, Marilene Silva; Rodrigues, Andreia C.M.; Dornelas, Aline Silvestre Pereira; Sarmento, Renato Almeida; Souza, João Carlos Perbone de; Resende, Erika Crispim; Saraiva, Althiéris S.

doi:10.3390/d14090698

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…Fipronil and cypermethrin are not used in rice cultivation every year, but studies show that their impact on health and freshwater ecotoxicity is quite high compared to other substances in this study, and they are other substances that should be considered if needed in the future. Fipronil can cause neurodegenerative disorders [58] and also affect aquatic ecosystems by affecting the behavior, regeneration, and reproduction of Girardia tigrina in water when exposed to chronic exposure [59]. In addition, cypermethrin ecotoxicity demonstrates potential hazards on several fish species (e.g., neurotoxicity, endocrine disruption, effects on proximate body composition, and oxidative stress injury) [60].…”

Section: Human and Ecotoxicological Impacts Under Different Rice Cult...mentioning

confidence: 99%

Environmental, Human and Ecotoxicological Impacts of Different Rice Cultivation Systems in Northern Thailand

Toolkiattiwong

Arunrat

Sereenonchai

2023

IJERPH

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Sustainable practices in rice cultivation require effective farming management concerning environmental and human health impacts. In this study, three rice cultivation systems, namely low-land, upland, and terraced rice in the Mae Chaem District, Chiang Mai Province, were assessed and the carbon footprint (CF), water footprint (WF), and human and ecotoxicological impacts were compared from pesticide application. The results showed that the highest CF intensity was observed in terraced rice with 1.15 kg CO2eq kg−1 rice yield, followed by lowland rice (1.02 kg CO2eq kg−1 rice yield) and upland rice (0.17 kg CO2eq kg−1 rice yield) fields. Moreover, lowland rice cultivation generated the highest total WF with 1701.6 m3 ton−1, followed by terraced rice (1422.1 m3 ton−1) and upland rice (1283.2 m3 ton−1). The lowland rice fields had the most impact on human health and freshwater ecotoxicity, followed by the terraced and upland rice cultivation systems. The results also showed that most of the pesticides remaining in soils were chlorpyrifos (98.88%), butachlor (96.94%), and fipronil (95.33%), respectively. The substances with the greatest distributions in freshwater were acephate (56.74%), glyphosate (50.90%), and metaldehyde (45.65%), respectively. This study indicated that, with more agricultural inputs, higher CF, WF, human health impacts, and freshwater ecotoxicity were generated. Although the use of pesticides in the study areas did not exceed the recommendations on the packaging, glyphosate and chlorpyrifos are restricted in Thailand, so it is necessary to monitor their use due to their long-term health effects.

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Section: Human and Ecotoxicological Impacts Under Different Rice Cult...mentioning

confidence: 99%

Environmental, Human and Ecotoxicological Impacts of Different Rice Cultivation Systems in Northern Thailand

Toolkiattiwong

Arunrat

Sereenonchai

2023

IJERPH

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“…L − 1 , there was a reduction in locomotor speed and a delay in the regeneration of the planarians, respectively. Planarians were less sensitive to pesticides Regent 800 WG® (LOEC 6.25 mg.L − 1 ) [33], and chlorantraniliprole (LOEC 131.7 µg.L − 1 ) [34], in the locomotion test, when compared to chlorpyrifos. Moreover, they proved to be more sensitive than tadpoles (Rhinella arenarum), these organisms when exposed to Chlorpyrifos had a decrease of locomotor performance in approximately 50% at the maximum concentration of Chlorpyrifos (75 µg i.a.…”

Section: Discussionmentioning

confidence: 99%

Environmentally relevant concentrations of chlorpyrifos affect behavior and physiological responses of Girardia tigrina

Saraiva

Reis

Farnese

et al. 2023

Preprint

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Chlorpyrifos, an organophosphate insecticide widely used to control agricultural pests, poses a significant environmental threat due to its toxicity and persistence in soil and water, causing deleterious effects on non-target organisms. In this context, our work aimed to evaluate the acute (survival) and chronic (regeneration, locomotion and reproduction) toxicity of chlorpyrifos in tropical freshwater planarians Girardia tigrina. The 48h lethal concentration (LC50) of the commercial formulation, containing 480 g L− 1 of chlorpyrifos active ingredient, was determined to be 622.8 µg a.i. L− 1 for planarians. Sublethal effects were also observed, with a significant reduction in locomotion and delayed head regeneration (equal lowest observed effect concentration – LOEC = 3.88 µg a.i. L− 1). Our study highlight the importance of employing physiological and behavioral parameters as effective tools for assessing the ecotoxicological impact of organophosphate insecticides on non-target tropical freshwater organisms, such as G tigrinia. Overall, this study demonstrates the potential of chlorpyrifos-based insecticides to harm natural populations of freshwater planarians at environmentally relevant concentrations. The observed toxicity serves as a cause for concern, emphasizing the need for stricter regulations and careful management of chlorpyrifos usage to mitigate its deleterious effects on aquatic ecosystems. By understanding the specific impacts on non-target organisms like G. tigrina, we can make more informed decisions regarding using and regulating organophosphate insecticides, ultimately promoting sustainable agricultural practices and environmental conservation.

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“…[14] Fipronil is a large-scale pesticide used to control pests in various crop fields. [21] In addition, this chemical has been used to control veterinary pests in cattle and even in domestic animals. [22,23] Fipronil is an insecticide that blocks GABA receptors from changing neural signaling in target organisms, [24] leading to their death.…”

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confidence: 99%

Cellular and molecular effects of fipronil in lipid metabolism of HepG2 and its possible connection to non‐alcoholic fatty liver disease

Pansa,

Molica,

de Oliveira Júnior

et al. 2023

J Biochem & Molecular Tox

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Nonalcoholic fatty liver disease (NAFLD) is a global public health problem that affects more than a quarter of the population. The development of this disease is correlated with metabolic dysfunctions that lead to lipid accumulation in the liver. Pesticides are one of etiologies that support NAFLD establishment. Therefore, the effects of the insecticide fipronil on the lipid metabolism of the human hepatic cell line, HepG2, was investigated, considering its widespread use in field crops and even to control domestic pests. To address the goals of the study, biochemical, cellular, and molecular analyses of different concentrations of fipronil in cell cultures were investigated, after 24 h of incubation. Relevant metabolites such as triglycerides, glucose levels, β‐oxidation processes, and gene expression of relevant elements correlated with lipid and metabolism of xenobiotics were investigated. The results suggested that at 20 μM, the pesticide increased the accumulation of triglycerides and neutral lipids by reducing fatty acid oxidation and increasing de novo lipogenesis. In addition, changes were observed in genes that control oxidative stress and the xenobiotic metabolism. Together, the results suggest that the metabolic changes caused by the insecticide fipronil may be deleterious if persistent, favoring the establishment of hepatic steatosis.

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Responses of Freshwater Planarian Girardia tigrina to Fipronil-Based Insecticide: Survival, Behavioral and Physiological Endpoints

Cited by 5 publications

References 48 publications

Environmental, Human and Ecotoxicological Impacts of Different Rice Cultivation Systems in Northern Thailand

Environmental, Human and Ecotoxicological Impacts of Different Rice Cultivation Systems in Northern Thailand

Environmentally relevant concentrations of chlorpyrifos affect behavior and physiological responses of Girardia tigrina

Cellular and molecular effects of fipronil in lipid metabolism of HepG2 and its possible connection to non‐alcoholic fatty liver disease

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