Responses of Gram-negative bacteria to certain environmental stressors

Ramos, Juan L.; Gallegos, Marı́a-Trinidad; Marqués, Silvia; Ramos-González, Marı́a Isabel; Espinosa‐Urgel, Manuel; Segura, Ana

doi:10.1016/s1369-5274(00)00183-1

Cited by 193 publications

(155 citation statements)

References 49 publications

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“…Multiple stress-related proteins, such as AphC, SodB and other antioxidants, are induced in P. putida KT2440 cells in the presence of a range of aromatic substrates (Kim et al, 2006). The presence of stress-related proteins in cells grown on styrene supports the claim that styrene has a toxic effect on bacteria cells (Ramos et al, 2001). Bacteria accumulating PHB have been previously reported to upregulate heat shock (HspA) proteins in response to stress (Kang et al, 2008;Tessmer et al, 2007).…”

Section: Stress-related Proteinsmentioning

confidence: 68%

“…On the one hand, styrene represents a carbon source that can be metabolized to yield carbon and energy for growth. On the other hand, with an octanol/water partition coefficient [log(P OW )] of 3, styrene falls into a category of organic solvents extremely toxic to microorganisms, due to their ability to bind to the cells, disturbing membranes by removing lipids and proteins and causing cell lysis (Ramos et al, 2001;Taylor et al, 2008). It is well documented that solvent-tolerant pseudomonads possess three membrane-associated solvent tolerance mechanisms: (A) active efflux of organic solvents, (B) outer membrane changes and (C) cytoplasmic membrane changes (Volkers et al, 2006).…”

Section: Mclpha Synthesis Occurs Only Under N Limitationmentioning

confidence: 99%

“…Several laboratories have identified efflux pumps belonging to the resistancenodulation-cell division (RND) family as being the most important mechanism of solvent tolerance (Kieboom et al, 1998;Ramos et al, 2001). They are energy-dependent active efflux pumps, which export toxic organic solvents to the external medium.…”

Section: Mclpha Synthesis Occurs Only Under N Limitationmentioning

confidence: 99%

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Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

et al. 2009

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Pseudomonas putida CA-3 is a styrene-degrading bacterium capable of accumulating mediumchain-length polyhydroxyalkanoate (mclPHA) when exposed to limiting concentrations of a nitrogen source in the growth medium. Using shotgun proteomics we analysed global proteome expression in P. putida CA-3 supplied with styrene as the sole carbon and energy source under N-limiting (condition permissive for mclPHA synthesis) and non-limiting (condition non-permissive for mclPHA accumulation) growth conditions in order to provide insight into the molecular response of P. putida CA-3 to limitation of nitrogen when grown on styrene. A total of 1761 proteins were identified with high confidence and the detected proteins could be assigned to functional groups including styrene degradation, energy, nucleotide metabolism, protein synthesis, transport, stress response and motility. Proteins involved in the upper and lower styrene degradation pathway were expressed throughout the 48 h growth period under both nitrogen limitation and excess. Proteins involved in polyhydroxyalkanoate (PHA) biosynthesis, nitrogen assimilation and amino acid transport, and outer membrane proteins were upregulated under nitrogen limitation. PHA accumulation and biosynthesis were only expressed under nitrogen limitation. Nitrogen assimilation proteins were detected on average at twofold higher amounts under nitrogen limitation. Expression of the branched-chain amino acid ABC transporter was up to 16-fold higher under nitrogen-limiting conditions. Branched chain amino acid uptake by nitrogenlimited cultures was also higher than that by non-limited cultures. Outer membrane lipoproteins were expressed at twofold higher levels under nitrogen limitation. This was confirmed by Western blotting (immunochemical detection) of cells grown under nitrogen limitation. Our study provides the first global description of protein expression changes during growth of any organism on styrene and accumulating mclPHA (nitrogen-limited growth). INTRODUCTIONPseudomonas putida CA-3 has been reported to utilize styrene as a sole source of carbon and energy and to accumulate the biodegradable polymer medium-chainlength polyhydroxyalkanoate (mclPHA) when nitrogen (N) becomes limited in the growth medium (O'Connor et al., 1996;. These two abilities have been employed in the two-step chemo-biotechnological conversion of polystyrene, a non-biodegradable polymer, to mclPHA at laboratory scale (Ward et al., 2006). The process has been improved through the controlled feeding of N to the growth medium, which results in a twofold increase in biomass and a 1.4-fold increase in mclPHA accumulation (Goff et al., 2007).Molecular investigations of the styrene degradation pathway in this organism have been reported (Mooney et al., 2006b;O'Connor et al., 2001). Styrene metabolism in P. putida CA-3 proceeds via initial side chain oxidation and involves an upper pathway converting styrene to phenylacetic acid (PA) (O'Connor et al., 1995), and a lower pathway initiated via activation of PA to phenylace...

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Section: Stress-related Proteinsmentioning

confidence: 68%

Section: Mclpha Synthesis Occurs Only Under N Limitationmentioning

confidence: 99%

Section: Mclpha Synthesis Occurs Only Under N Limitationmentioning

confidence: 99%

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Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

et al. 2009

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“…As bactérias estão sujeitas a uma série de alterações ambientais, como temperatura, disponibilidade de nutrientes e água, presença de moléculas tóxicas, até mesmo deletérias que se originam do próprio metabolismo e que podem resultar em condições adversas de crescimento (RAMOS et al, 2001). Um dos mecanismos desenvolvidos por bactérias para sobreviver em tais situações é a capacidade de responder ao estresse sintetizando diferentes moléculas que favorecem o seu desenvolvimento nessas condições adversas.…”

Section: Papel Do Estresse Na Interação Planta-microrganismounclassified

“…Esse aumento direciona a RNA polimerase a transcrever mais de 20 genes específicos codificantes de HSPs. A indução de resposta à temperatura está conectada a processos metabólicos específicos na célula (RAMOS et al, 2001).…”

Section: Papel Do Estresse Na Interação Planta-microrganismounclassified

Interação entre Methylobacterium extorquens e cana-de-açúcar (Saccharum sp.)

Silva¹

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AGRADECIMENTOSÀ Deus, por me guiar e me dar coragem para superar os momentos difíceis; À meus pais e meus irmãos por todo apoio, amor e compreenção em mais esta etapa da minha vida; Ao Dr. Welington Luiz de Araújo, pela orientação, apoio e amizade nesses dois anos de trabalho; À Profa. Dra. Aline Aparecida Pizzirani-Kleiner e prof. Dr. João Lúcio de Azevedo, pela amizade e receptividade; Ao Prof. Dr. Carlos Alberto Labate, pela disponibilização integral da excelente infra estrutura de seu laboratório, indispensáveis a realização deste trabalho; Ao Departamento de Genética da ESALQ/USP, funcionários e colegas, que foram importantes em algum passo para a realização deste trabalho;Aos coordenadores e professores do curso de pós graduação em Genética e Melhoramento de por RAPD ............................................................................................................31 parcial do 16S rDNA ...................................................................... ...........................................................................................................33 do extrato proteíco.............................................................................. ......................................................................................................................... em gel de poliacrilamida .........................................................................35 de imagem dos géis...................................................................................... extorquens na planta hospedeira .......................................................36 M. extorquens .......................................................................................36 M. extorquens ............................................... As plantas quando colonizadas produzem diversas enzimas de defesa que podem impedir o estabelecimento de microrganismos. Esta condição adversa na planta hospedeira gera uma resposta do microrganismo, a qual está associada à síntese de proteínas e outras moléculas que atuam na sua interação com a planta e alteram a comunidade microbiana associada. Todos os organismos respondem a essa condição, estabelecendo biofilmes, ou sintetizando um grupo de moléculas e proteínas que os protegem de danos e facilita a recuperação. Estas são chamadas proteínas de choque térmico (HSPs), as quais não foram ainda estudadas na interação Methylobacterium -planta. As bactérias do gênero Methylobacterium são metilotróficas facultativas da classe Alfa-proteobactéria, encontradas em relações epifíticas e endofíticas com diferentes espécies vegetais. Assim, o presente trabalho teve como objetivos avaliar o efeito da deficiência da produção de biofilme e de Acil-Homoserina-Lactonas (AHLs), e do estresse térmico de M. extorquens na colonização da planta hospedeira (Saccharum sp.). Para isso, foram usadas linhagens defectivas para produção de biofilme e AHLs, juntamente com a linhagem selvagem submetida ou não ao estresse térmico. Os resultados obtidos mostram a complexidade dos mecanismos envo...

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Desert Environments: Soil Microbial Communities in Hot Deserts

Kieft

2003

Encyclopedia of Environmental Microbiology

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Soil Water Potential Desert Soil Microbial Abundance Spatial and Temporal Distributions Diversity of Desert Soil Microbes Adaptations to Desert Soil Conditions Practical Applications

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Responses of Gram-negative bacteria to certain environmental stressors

Cited by 193 publications

References 49 publications

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

Interação entre Methylobacterium extorquens e cana-de-açúcar (Saccharum sp.)

Desert Environments: Soil Microbial Communities in Hot Deserts

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