Responses of immature dental pulp cells to hypoxic stimulation

Senzui, Sachie; Matsuzaka, Kenichi; Fukuhara, Fumiko; Shintani, Seikou; Inoue, Takashi

doi:10.3353/omp.14.107

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…) and to affect the differentiation ability of odontoblasts (Senzui et al . ). In addition, not only dental pulp cells but also periodontal ligament cells maintain cell homeostasis during hypoxia and reoxygenation and promote cell proliferation and differentiation under hypoxic conditions in vitro (Amemiya et al .…”

Section: Introductionmentioning

confidence: 97%

“…In dental pulp cells studied in vitro, hypoxia was shown to be an effective treatment to amplify numbers of progenitor cells (Sakdee et al 2009), to enhance angiogenic potential (Aranha et al 2010) and to affect the differentiation ability of odontoblasts (Senzui et al 2010). In addition, not only dental pulp cells but also periodontal ligament cells maintain cell homeostasis during hypoxia and reoxygenation and promote cell proliferation and differentiation under hypoxic conditions in vitro (Amemiya et al 2003(Amemiya et al , 2008.…”

Section: Introductionmentioning

confidence: 99%

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Hypoxic condition promotes differentiation and mineralization of dental pulp cells in vivo

et al. 2014

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Hypoxic condition promotes differentiation and mineralization of dental pulp cells in vivo

et al. 2014

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“…Thus, it is plausible that the ambient O 2 tension might not be suitable for the establishment and maintenance of DPSCs. In that context, some studies demonstrated that cultivation of DPSCs under hypoxic condition (1–3%) enhances proliferation potential 41 and angiogenic potential 42 , and affects the odontoblastic differentiation potential 43 .…”

mentioning

confidence: 99%

The effects of hypoxia on the stemness properties of human dental pulp stem cells (DPSCs)

Ahmed

Murakami

Kaneko

et al. 2016

Sci Rep

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Recent studies have demonstrated that culture under hypoxia has beneficial effects on mesenchymal stem cells (MSCs). However, there are limitations to achieving a stable condition in conventional hypoxic CO2 incubators. DPSCs are a unique type of MSCs which are promising in many regenerative therapies. In this study, we investigated the ideal hypoxic culture environment for DPSCs using a new system that can provide controlled O2 environment. The effects of hypoxia (3%, 5%) on the stemness properties of DPSCs. Their morphology, proliferation rate, expression of stem cell markers, migration ability, mRNA expression of angiogenic/neurotrophic factors and immunomodulatory genes were evaluated and compared. Additionally, the effect of the discrete secretome on proliferation, migration, and neurogenic induction was assessed. Hypoxic DPSCs were found to be smaller in size and exhibited larger nuclei. 5% O2 significantly increased the proliferation rate, migration ability, expression of stem cell markers (CXCR4 and G-CSFR), and expression of SOX2, VEGF, NGF, and BDNF genes of DPSCs. Moreover, secretome collected from 5%O2 cultures displayed higher stimulatory effects on proliferation and migration of NIH3T3 cells and on neuronal differentiation of SH-SY5Y cells. These results demonstrate that 5%O2 may be ideal for enhancing DPSCs growth, stem cell properties, and secretome trophic effect.

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“…For the hypoxic condition group, cells were incubated in a humidified atmosphere at conditions of 5% O 2 , 5% CO 2 and 90% N 2 at 37˚C. For the normal condition group, cells were incubated in a humidified atmosphere at normal conditions of 20% O 2 , 5% CO 2 and 75% N 2 at 37˚C (15).…”

Section: Osteogenic Differences Inmentioning

confidence: 99%

Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions

Ichijima

Matsuzaka

Tonogi

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the osteogenic capability of rat calvarial periosteal cells in hypoxic conditions in vitro. Periosteum was obtained from the calvarial bone of Sprague-Dawley rats. Following primary tissue culture, subcultured cells were used in hypoxic or normal conditions. On days 1, 2, 3 and 4 following the cell culture, cell proliferation and mRNA and protein expression levels were evaluated. No significant difference in the cell proliferation rate was found between the normal and hypoxic condition groups. The hypoxic condition group exhibited a stronger expression of hypoxia-inducible factor (HIF)1α, vascular endothelial growth factor (VEGF), Runx2, alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN) and periostin at the mRNA level compared to that of the normal condition group. The hypoxic condition group also exhibited a stronger expression of HIF1α, VEGF, bone morphogenetic protein (BMP)2, Runx2, ALP and BSP at the protein level compared to that of the normal condition group. In conclusion, periosteal cells cultured in hypoxic conditions demonstrated activated osteogenic capability in vitro.

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Responses of immature dental pulp cells to hypoxic stimulation

Cited by 5 publications

References 17 publications

Hypoxic condition promotes differentiation and mineralization of dental pulp cells in vivo

Hypoxic condition promotes differentiation and mineralization of dental pulp cells in vivo

The effects of hypoxia on the stemness properties of human dental pulp stem cells (DPSCs)

Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions

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