Responses of marine mussel Mytilus galloprovincialis (Bivalvia: Mytilidae) after infection with the pathogen Vibrio splendidus

Parisi, Maria Giovanna; Cappello, Tiziana; Oliva, Sabrina; Mauceri, Angela; Toubiana, Mylène; Cammarata, Matteo

doi:10.1016/j.cbpc.2019.03.005

Cited by 20 publications

(10 citation statements)

References 52 publications

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“…However, V. splendidus clade bacteria have a wide range of bivalve mollusk hosts, such as scallops, mussels, and clams, causing similar harmful effects as in oysters [ 55 , 56 , 57 , 58 , 59 , 60 ]. Vibriosis in bivalve mollusks, caused by bacteria belonging to the splendidus clade, is associated with histopathological lesions, such as the disorganization of muscle fibers and strong inflammatory responses, as well as the general depression of the physiology [ 56 , 61 ]. Another pathogenic agent, belonging to Vibrionaceae family, causing mortalities in a wide variety of aquatic animals is V. harveyi , although it has been shown to belong to the microbiome of most of the infected hosts [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

Lattos

Bitchava²,

Giantsis

et al. 2021

Microorganisms

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Pinna nobilis populations, constituting the largest bivalve mollusk endemic to the Mediterranean, is characterized as critically endangered, threatened by extinction. Among the various factors proposed as etiological agents are the Haplosporidium pinnae and Mycobacterium sp. parasites. Nevertheless, devastation of the fan mussel populations is still far from clear. The current work is undertaken under a broader study aiming to evaluate the health status of Pinna nobilis population in Aegean Sea, after the mass mortalities that occurred in 2019. A significant objective was also (a) the investigation of the etiological agents of small-scale winter mortalities in the remaining populations after the devastating results of Haplosporidium pinnae and Mycobacterium sp. infections, as well as (b) the examination of the susceptibility of the identified bacterial strains in antibiotics for future laboratory experiments. Microbiological assays were used in order to detect the presence of potential bacterial pathogens in moribund animals in combination with molecular tools for their identification. Our results provide evidence that Vibrio bacterial species are directly implicated in the winter mortalities, particularly in cases where the haplosporidian parasite was absent. Additionally, this is the first report of Vibrio mediterranei and V. splendidus hosted by any bivalve on the Greek coastline.

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Section: Discussionmentioning

confidence: 99%

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

Lattos

Bitchava²,

Giantsis

et al. 2021

Microorganisms

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“…Exposure of C. gigas embryos and larvae to the gram-negative bacteria Vibrio coralliilyticus has shown that apoptotic responses to pathogens develop early in oyster larvae (blastula -D-shelled larvae) near the digestive gland with rst Cg3B appearing in D-larvae 12 h post exposure to bacteria (34). V. splendidus also increased caspase-3 activity in the posterior adductor muscle in M. galloprovincialis 24-72 h post injection (84). Furthermore, Vibrio alginolyticus increases the expression of Ch8A and Ch3/7 in haemocytes of C. hongkongensis oysters (25,31).…”

Section: Caspases In Bivalve Development Immune System Function Andmentioning

confidence: 99%

Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development

Vogeler

Carboni

et al. 2020

Preprint

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Background: Apoptosis is an important process for an organism’s innate immune system to respond to pathogens, while also allowing for cell differentiation and other essential life functions. Caspases are one of the key protease enzymes involved in the apoptotic process, however there is currently a very limited understanding of bivalve caspase diversity and functions. Results: In this work, we investigated the presence of caspase homologs using a combination of bioinformatics and phylogenetic analyses. We blasted the Crassostrea gigas genome for caspase homologs and identified thirty-five potential homologs in the addition to the already cloned twenty-three bivalve caspases. As such, we present information about the phylogenetic relationship of all identified bivalve caspases in relation to their homology to well-established vertebrate and invertebrate caspases. Our results reveal unexpected novelty and complexity in the bivalve caspase family. Notably, we were unable to identify direct homologues to the initiator caspase-9, a key-caspase in the vertebrate apoptotic pathway, inflammatory caspases (caspase-1,-4 or -5) or executioner caspases-3, -6, -7. We also explored the fact that bivalves appear to possess several unique homologs to the initiator caspase groups -2 and -8. Large expansions of caspase-3 like homologues (caspase-3A-C), caspase-3/7 group and caspase-3/7-like homologues were also identified, suggesting unusual roles of caspases with direct implications for our understanding of immune response in relation to common bivalve diseases. Furthermore, we assessed the gene expression of two initiator (Cg2A, Cg8B) and four executioner caspases (Cg3A, Cg3B, Cg3C, Cg3/7) in C. gigas late-larval development and during metamorphosis, indicating that caspase expressions vary across the different developmental stages.Conclusion: Our analysis provides the first overview of caspases across different bivalve species with essential new insight into caspase diversity, knowledge that can be used for further investigations into response to pathogens or regulation of developmental processes.

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“…It is well known that environmental chemicals usually affect the Na + /K + -ATPase enzyme (Begum, 2011). In bivalve molluscs, Na + /K + -ATPase has a significant role in regulating intracellular Na + concentration in response to different environmental conditions (Parisi et al 2019). Because membrane fluidity allows membrane-bound proteins such as Na + /K + -ATPase to function, changes in the lipid composition and structure of the membrane may cause a decrease in Na + /K + -ATPase activity (Pałecz, Komuński, & Gabryelak, 2005).…”

Section: Atpase Activitiesmentioning

confidence: 99%

“…Bivalves, especially mussels, have various characteristics such as a wide geographic distribution, sessile life, deep burrowing, accumulation of many types of pollutants, and a high tolerance for chemical pollution (Okay et al, 2016;Burgos-Aceves, & Faggio, 2017;Guidi et al, 2017;Savorelli et al, 2017;Sureda, Capó, Busquets-Cortés, & Tejada, 2018). They accumulate pollutants in the surrounding water due to their filter-feeding characteristics (Parisi et al, 2019). In the present study, the Unio mancus Lamarck, 1819 commonly found in the Atatürk Dam Lake was chosen as a biomonitor species.…”

Section: Introductionmentioning

confidence: 99%

Assesment of Na+/K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and Total-ATPase Activities in Gills of Freshwater Mussels Exposed to Penconazole

Yoloğlu

2019

Commagene Journal of Biology

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Penconazole, a triazole fungicide, is widely used worldwide to increase agricultural production and to control fungus. Bivalves, especially mussels, are specific biomonitor organisms of pollution in aquatic ecosystems. This study deals with the possible toxic effects of exposure to penconazole on Na + /K + -ATPase, Mg 2+ -ATPase, Ca 2+ -ATPase, and Total-ATPase activities in the gills of freshwater mussels (Unio mancus). In this context, freshwater mussels were exposed to different concentrations of penconazole (1, 10, 100, and 1000 µg AI L -1 ) in 96 hours static-renewal test system. The commercial form of penconazole (TOPAS ® 100 EC, Syngenta, Germany) was used in toxicity tests. The actual penconazole concentrations in the exposure media were determined by LC-MS/MS analysis. According to the LC-MS/MS analysis results, the actual penconazole concentrations in the exposure media were determined to be approximately 25% lower than the nominal penconazole concentrations. After 96 hours of exposure, the highest concentration of penconazole caused significant inhibition of ATPase activities except Mg 2+ -ATPase activity (P < 0.05). The observed data indicated that acute penconazole exposure may be a potential risk for freshwater mussels and that ATPase enzymes are important and useful biochemical markers for aquatic toxicology studies.Öz: Triazol bir fungusit olan penkonazol, tarımsal üretimi arttırmak ve fungusları kontrol etmek için dünya çapında yaygın olarak kullanılmaktadır. Çift kabuklu yumuşakçalar, özellikle midyeler, sucul ekosistemlerde kirliliğin spesifik biyomonitor organizmalarıdır. Bu çalışmada tatlı su midyelerinin (Unio mancus) solungaç Na + /K + -ATPaz, Mg 2+ -ATPaz, Ca 2+ -ATPaz ve Total-ATPaz aktiviteleri üzerine penkonazol maruziyetinin olası toksik etkileri değerlendirilmiştir. Bu amaçla, 96 saatlik statik yenileme test sisteminde tatlı su midyeleri farklı konsantrasyonlarda penkonazole (1, 10, 100 ve 1000 µg AI L -1 ) maruz bırakılmıştır. Toksisite testlerinde penkonazolün ticari formu (TOPAS ® 100 EC, Syngenta, Almanya) kullanılmıştır. Maruziyet ortamındaki gerçek penkonazol konsantrasyonları LC-MS/MS analizi ile belirlenmiştir. LC-MS/MS analizi sonuçlarına göre, maruziyet ortamındaki gerçek penkonazol konsantrasyonlarının, nominal penkonazol konsantrasyonlarından yaklaşık %25 oranında daha düşük olduğu belirlenmiştir. 96 saatlik maruziyetten sonra, en yüksek penkonazol konsantrasyonu Mg 2+ -ATPaz hariç diğer ATPaz aktivitelerinin önemli düzeyde inhibisyonuna neden olmuştur (P < 0.05). Elde edilen veriler, akut penconazol maruziyetinin tatlı su midyeleri için potansiyel bir risk olabileceğini ve ATPaz enzimlerinin akuatik toksikoloji çalışmaları için önemli ve faydalı biyokimyasal belirteçler olduğunu göstermiştir.Anahtar kelimeler: Penkonazol toksisitesi, tatlı su midyesi, ATPazlar, LC-MS/MS.

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Responses of marine mussel Mytilus galloprovincialis (Bivalvia: Mytilidae) after infection with the pathogen Vibrio splendidus

Cited by 20 publications

References 52 publications

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

The Implication of Vibrio Bacteria in the Winter Mortalities of the Critically Endangered Pinna nobilis

Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development

Assesment of Na+/K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and Total-ATPase Activities in Gills of Freshwater Mussels Exposed to Penconazole

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