Responses of Microbial Communities to Hydrocarbon Exposures

Joye, Samantha B.; Kleindienst, Sara; Gilbert, Jack A.; Handley, Kim M.; Weisenhorn, Pamela; Overholt, Will A.; Kostka, Joel E.

doi:10.5670/oceanog.2016.78

Cited by 65 publications

(51 citation statements)

References 70 publications

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“…and Sulfitobacter spp. that have been found to be enriched in oil-amendments performed using sub-ice Arctic seawater (Garneau et al, 2016), and in beach sands of the Gulf of Mexico following the Deep Horizon or other oil spills (Rodriguez-R et al, 2015;Joye et al, 2016). Our results indicate that background levels of OPs substantially stimulate the growth of OP-degrading bacteria from the rare biosphere.…”

Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 66%

“…In Antarctica, at the final time point, they accounted for 0.2% (AE 0.16%) of the total community, suggesting a slower or weaker response to ADOC than the one observed in Arctic waters (Supporting Information Fig. that have been found to be enriched in oil-amendments performed using sub-ice Arctic seawater (Garneau et al, 2016), and in beach sands of the Gulf of Mexico following the Deep Horizon or other oil spills (Rodriguez-R et al, 2015;Joye et al, 2016). Interestingly, in the Arctic, most of the members of the ADOC-enriched taxa corresponded to taxa belonging to the genera Nocardioides, Pseudomonas and Sphingobium, such as Sphingobium japonicum UT26 and Nocardioides sp.…”

Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 97%

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Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Cerro‐Gálvez

Casal

Lundin

et al. 2019

Environmental Microbiology

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Summary Thousands of semi‐volatile hydrophobic organic pollutants (OPs) reach open oceans through atmospheric deposition, causing a chronic and ubiquitous pollution by anthropogenic dissolved organic carbon (ADOC). Hydrophobic ADOC accumulates in cellular lipids, inducing harmful effects on marine biota, and can be partially prone to microbial degradation. Unfortunately, their possible effects on microorganisms, key drivers of global biogeochemical cycles, remain unknown. We challenged coastal microbial communities from Ny‐Ålesund (Arctic) and Livingston Island (Antarctica) with ADOC concentrations within the range of oceanic concentrations in 24 h. ADOC addition elicited clear transcriptional responses in multiple microbial heterotrophic metabolisms in ubiquitous groups such as Flavobacteriia, Gammaproteobacteria and SAR11. Importantly, a suite of cellular adaptations and detoxifying mechanisms, including remodelling of membrane lipids and transporters, was detected. ADOC exposure also changed the composition of microbial communities, through stimulation of rare biosphere taxa. Many of these taxa belong to recognized OPs degraders. This work shows that ADOC at environmentally relevant concentrations substantially influences marine microbial communities. Given that emissions of organic pollutants are growing during the Anthropocene, the results shown here suggest an increasing influence of ADOC on the structure of microbial communities and the biogeochemical cycles regulated by marine microbes.

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Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 66%

Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 97%

Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Cerro‐Gálvez

Casal

Lundin

et al. 2019

Environmental Microbiology

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“…Lack of baseline data has been repeatedly identified as a major obstacle in assessing the ecological damage and recovery of the Gulf of Mexico following DWH, particularly where ultra‐deep drilling is now occurring (Joye et al . ). Such data are likewise generally unavailable for other parts of the world ocean where oil and gas exploration and drilling are ongoing including the FSC.…”

Section: Discussionmentioning

confidence: 97%

“…The detection and identification of key micro‐organisms that respond to hydrocarbon inputs is the first step towards a site‐specific assessment of the bioremediation potential and appropriate response strategies in the event of anthropogenic hydrocarbon discharges (Joye et al . ). The aim of this study was to provide baseline information on the identity of key hydrocarbon‐degrading bacteria in sediments of the Faroe‐Shetland Channel (FSC), an active area of oil exploration and planned production at depths of 1100 m on the continental shelf of the United Kingdom (UKCS) (Austin et al …”

Section: Introductionmentioning

confidence: 97%

Hydrocarbon-degrading bacteria in deep-water subarctic sediments (Faroe-Shetland Channel)

et al. 2018

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“…Oil pollution in the marine environment quickly triggers a number of microbial responses, including a broad range of activities: (1) chemotaxis, either away from toxic hydrocarbons or towards them, (2) efflux mechanisms to facilitate secretion of small hydrocarbons that may enter bacterial cells by passive transport, together with secretion systems to transport, proteins (toxins and extracellular enzymes) or biosurfactants outside the cell, (3) death and lysis of sensitive organisms which releases a pool of organic material available for the oil-tolerant and primary or secondary oil-degrading organisms, (4) adhesion to and biofilm formation on oil droplets and signaling between the biofilm forming cells, (5) an increase in nutrient transport and processes to generate available nitrogen, phosphorous and sulfur for growth on carbon-rich and nutrient-poor oil components, and (6) biosurfactant synthesis (Van Hamme, Singh et al 2003, Rivers, Sharma et al 2013, Joye, Kleindienst et al 2016, Parales and Ditty 2018. Indeed, most of these processes, also reported in recent large scale metatranscriptomic studies (Tremblay, Yergeau et al 2017, Tremblay, Fortin et al 2019, were represented in the upregulated genes in our dataset (Table S7).…”

Section: Upregulated Functionsmentioning

confidence: 99%

Discovery of functional gene markers of bacteria for monitoring hydrocarbon pollution in the marine environment - a metatranscriptomics approach

Knapik

Bagi

Królicka

et al. 2019

Preprint

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The use of natural marine bacteria as "oil sensors" for the detection of pollution events can be suggested as a novel way of monitoring oil occurrence at sea. Nucleic acid-based devices generically called genosensors are emerging as potentially promising tools for in situ detection of specific microbial marker genes suited for that purpose. Functional marker genes are particularly interesting as targets for oil-related genosensing but their identification remains a challenge. Here, seawater samples, collected in tanks with oil addition mimicking a realistic oil spill scenario, were filtered and archived by the Environmental Sample Processor (ESP), a fully robotized genosensor, and the samples were then used for post-retrieval metatranscriptomic analysis. After extraction, RNA from ESP-archived samples at start, day 4 and day 7 of the experiment was used for sequencing. Metatranscriptomics revealed that several KEGG pathways were significantly enriched in samples exposed to oil. However, these pathways were highly expressed also in the non-oil-exposed water samples, most likely as a result of the release of natural organic matter from decaying phytoplankton. Temporary peaks of aliphatic alcohol and aldehyde dehydrogenases and monoaromatic ringdegrading enzymes (e.g. ben, box, and dmp clusters) were observed on day 4 in both control and oil tanks. Few alkane 1-monooxygenase genes were upregulated on oil, mostly transcribed by families Porticoccaceae and Rhodobacteraceae, together with aromatic ring-hydroxylating dioxygenases, mostly transcribed by Rhodobacteraceae. Few transcripts from obligate hydrocarbonoclastic genera of Alcanivorax, Oleispira and Cycloclasticus, were significantly enriched in the oil-treated tank in comparison to control, and these were mostly transporters and genes involved in nitrogen and phosphorous acquisition. This study highlights the importance of seasonality, i.e., phytoplankton occurrence and senescence leading to organic compound release which can be used preferentially by bacteria over oil compounds, delaying the latter process. As a result, such seasonal effect can reduce the sensitivity of genosensing tools employing bacterial functional genes to sense oil. A better understanding of the use of natural organic matter by bacteria involved in oil-biodegradation is needed to develop an array of functional markers enabling the rapid and specific in situ detection of anthropogenic pollution.

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Responses of Microbial Communities to Hydrocarbon Exposures

Cited by 65 publications

References 70 publications

Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Hydrocarbon-degrading bacteria in deep-water subarctic sediments (Faroe-Shetland Channel)

Discovery of functional gene markers of bacteria for monitoring hydrocarbon pollution in the marine environment - a metatranscriptomics approach

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