Citrus seedlings sprayed with chemicals which influence the cold hardiness of other plants were hardened in controlled conditions. Maleic hydrazide (MH-30) increased cold hardiness; however, growth retardants (2-chloroethyl)trimethylammoniumchloride (chlormequat) and succinic acid-2,2-dimethylhydrazide (SADH), and growth inhibitor abscisic acid (ABA) did not. ABA at high concns decreased cold hardiness as did gibberellic acid (GA3). Benzyladenine (BA), kinetin (KN), decenylsuccinic acid (DSA), and (2-chloroethyl)phosphonic acid (ethephon) had little or no effect on cold hardiness. These results are consistent with tests on citrus conducted under field conditions.