Responses of Peanut Somatic Embryos to Thidiazuron

Joshi, M. S.; Sahasrabudhe, N.A.; Hazra, Sulekha

doi:10.1023/a:1022886107591

Cited by 18 publications

(4 citation statements)

References 24 publications

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“…In groundnut ( Arachis hypogaea L.), successful results were achieved via organogenesis (Tiwari & Tuli, ) and somatic embryogenesis (Joshi, Sahasrabudhe, & Hazra, ). Similar to soybean, growth regulators and type of explants are the key factors for groundnut regeneration with a strong influence of genotype (Matand & Prakash, ).…”

Section: In Vitro Regenerationmentioning

confidence: 99%

Potential, constraints and applications of in vitro methods in improving grain legumes

et al. 2018

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Grain legumes, the important constituents of sustainability-based cropping systems and energy-limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so-called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest.The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement.

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Section: In Vitro Regenerationmentioning

confidence: 99%

Potential, constraints and applications of in vitro methods in improving grain legumes

et al. 2018

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“…Проведенные исследования показывают, что возможность получения каллусов из зародышевых эксплантов арахиса зависит от генотипа используемых образцов, что соответствует результатам опытов других экспериментаторов (Baker et al, 1995;Joshi et al, 2003). Способность образуемых каллусов в наших экспериментах получено не было.…”

Section: таблица 3 динамика изменения среднего диаметра каллусаunclassified

Callus formation ability in cultivated peanuts (<i>Arachis hypogaea</i> L.)

Bemova

Макарова

Gurina

et al. 2022

Biotehnologiâ i selekciâ rastenij

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Background: Russia is one of the largest peanut importing countries. At the same time, in the south of the country, several zones meet the requirements for peanut cultivation. It is possible to increase the yield of the existing peanut varieties by using modern biotechnology methods, in particular agrobacterial transformation. It is known from the literature data that different peanut genotypes and explants from various sources react differently to in vitro regeneration. Successful regeneration depends on the correct protocol, including both the type of regeneration and the composition of media promoting growth and in vitro induction.Objectives: a technique for obtaining peanut regenerants in in vitro culture.Materials and methods: Eight peanut accessions from the VIR collection of different origin were used in the work. Embryonic explants were grown on Murashige-Skoog medium supplemented with the hormone 2,4-dichlorophenoxyacetic acid (2,4-D).Results and conclusions: As a result of assessing the regenerative ability of peanuts grown on Murashige-Skoog medium with the hormone 2,4-D at a concentration of 2 g/L, differences in the callus formation ability were revealed in different accessions. Those with catalog numbers k-793, k-2054 and k-2055 did not form organogenic calli, while accessions k-698 and k-1987 showed the highest percentage of callus formation from embryonic explants.

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“…In peanut epicotyl [23], hypocotyl [24], somatic embryos [25], and cotyledonary node [5,6,26,27] were reported effective in in vitro peanut regeneration. However, the plant variety, culture media, culture condition, and source of explant had been reported as the factors that influence tissue culture system or condition [28].…”

Section: Introductionmentioning

confidence: 99%

Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars

Lamboro¹,

Han²,

Yang³

et al. 2022

Phyton

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Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied to evaluate shoot regeneration capacity whereas α-naphthalene acetic acid (NAA) combination with 6-benzylaminopurine (BAP), and 2, 4-dichlorophenoxyacetic acid (2, 4-D) with 6-benzylaminopurine were tested to optimize root induction for two peanut cultivars. The result showed combination (BAP with TDZ) was found to be effective in promoting shoot. The highest shoot regeneration frequency (93%) was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L TDZ while an average regeneration frequency (87%) was achieved in a medium containing combinations of 2 mg/L BAP with 1 mg/L TDZ. The shooting rate increased for both cultivars as the concentrations of BAP increased and TDZ decreased. The highest rooting rate (93%) was obtained on a medium supplemented with 3.5 mg/L NAA with 2.5 mg/L BAP for both cultivars. The rooting rate increased as the concentration of auxin to cytokinin ratio increased. The maximum rooting rate (83%) was obtained on MS medium supplemented with 0.3 mg/L 2, 4-D with 0.2 mg/L BAP for the cultivar N3. The result indicated that BAP with NAA was much better than BAP with 2, 4-D in rooting rate. Thus, the protocol developed was genotype independent and effective for peanut tissue culture.

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Responses of Peanut Somatic Embryos to Thidiazuron

Cited by 18 publications

References 24 publications

Potential, constraints and applications of in vitro methods in improving grain legumes

Potential, constraints and applications of in vitro methods in improving grain legumes

Callus formation ability in cultivated peanuts (<i>Arachis hypogaea</i> L.)

Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars

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