Responses of peritoneal mesothelial cells in rats with aseptic and bacterial peritonitis

Morphology of the rat peritoneum after carbon dioxide and helium pneumoperitoneum: a scanning electron microscopic study

“…At variance with this interpretation, however, it is commonly assumed that inflammatory reaction patterns are uniform and not agent specific [11,13,17]. Furthermore, Suematsu et al do not explain why laparotomy, capnoperitoneum, or helium pneumoperitoneum should induce different alterations of the peritoneal morphology.…”

“…In experimental studies, similar alterations have been described in uremia, in the course of barium peritonitis, after exposition to starch, and after intraabdominal injection of cell-free cancerous ascites [7,13,14,17,24]. Characteristic features of bacterial peritonitis are the appearance of a fibrin network on the surface of the peritoneum and the intensive infiltration of the peritoneum by polymorph nuclear cells [13].…”

“…In general, the inflammatory reaction of the peritoneum to toxic agents is characterized by bulging and separation of mesothelial cells, and by exfoliation of the cells, resulting in the exposure of submesothelial connective tissue and subsequent infiltration of the peritoneum by leukocytes and macrophages [11,13].…”

“…Its acute inflammatory reaction to toxic agents is characterized by bulging and separation of mesothelial cells, and by exfoliation of the cells, resulting in the exposure of submesothelial connective tissue and subsequent infiltration of the peritoneum by leukocytes and macrophages within 12 to 24 h. Several authors support the hypothesis that these changes are not agent specific, but rather represent a uniform inflammatory reaction pattern of the peritoneum [11,13]. In experimental studies, similar alterations have been described in uremia, in the course of barium peritonitis, after exposition to starch, and after intraabdominal injection of cell-free cancerous ascites [7,13,14,17,24]. Characteristic features of bacterial peritonitis are the appearance of a fibrin network on the surface of the peritoneum and the intensive infiltration of the peritoneum by polymorph nuclear cells [13].…”

Application of stereology to study the effects of pneumoperitoneum on peritoneum

“…Hypoxia in tissues might exert the strongest effect on peritoneal cells, such as changing the microfilament-based cytoskeleton, and cellular ion and electrical potentials [24]. Several authors have supported the hypothesis that observed ultrastructural changes should not be attributed to the specific gas but rather to a uniform inflammatory reaction pattern of the peritoneum [25,26]. Our study found pneumoperitoneum with either CO 2 or He insufflation has equal impacts on peritoneal alterations caused by homeostatic alterations, hypoxia, and uniform inflammatory reaction pattern.…”