Responses of proliferating and non-proliferating Chinese hamster cells to cytotoxic agents

Epifanova, O. I.; Smolenskaya, I. N.; Polunovsky, Vitaly A.

doi:10.1038/bjc.1978.57

Cited by 15 publications

(3 citation statements)

References 21 publications

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“…It is well known that vigorously proliferating cells were more sensitive to natural product drugs or cytotoxic chemicals than nonproliferating/quiescent cells [35]. One possible reason for this is that most cytotoxic drugs targeted replicated DNA easily.…”

Section: Discussionmentioning

confidence: 99%

Hypericin-photodynamic therapy inhibits the growth of adult T-cell leukemia cells through induction of apoptosis and suppression of viral transcription

Zhang

Cheng

et al. 2019

Retrovirology

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Background Adult T-cell leukemia (ATL) is an aggressive neoplasm caused by human T-cell leukemia virus type 1 (HTLV-1). ATL carries a poor prognosis due to chemotherapy resistance. Thus, it is urgent to develop new treatment strategies. Hypericin (HY) is a new-type of photosensitizer in the context of photodynamic therapy (PDT) due to its excellent photosensitizing properties and anti-tumor activities. Results In the present study, we investigated the efficacy of hypericin in ATL cells. Clinically achievable concentrations of hypericin in association with PDT induced the inhibition of cell proliferation in ATL cell lines with minimal effect on peripheral blood CD4 + T lymphocytes. Moreover, hypericin-PDT treatment caused apoptosis and G2/M phase cell cycle arrest in leukemic cells. Western blot analyses revealed that hypericin-PDT treatment resulted in downregulation of Bcl-2 and enhanced the expression of Bad, cytochrome C, and AIF. Cleavage of caspases-3/-7/-9/-8, Bid, and PARP was increased in hypericin-PDT-treated ATL cells. In a luciferase assay, hypericin-PDT treatment was able to activate the promoter activity of Bax and p53, resulting in enhanced expression of Bax and p53 proteins. Finally, hypericin-PDT treatment suppressed the expression of viral protein HBZ and Tax by blocking the promoter activity via HTLV-1 5′LTR and 3′LTR. Conclusions Our results revealed that hypericin-PDT is highly effective against ATL cells by induction of apoptosis and suppression of viral transcription. These studies highlight the promising use of hypericin-PDT as a targeted therapy for ATL. Electronic supplementary material The online version of this article (10.1186/s12977-019-0467-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Hypericin-photodynamic therapy inhibits the growth of adult T-cell leukemia cells through induction of apoptosis and suppression of viral transcription

Zhang

Cheng

et al. 2019

Retrovirology

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show abstract

“…15,16 In the absence of these signals, cells undergo apoptosis 17 or exit the cell cycle and accumulate in the reversible phase of proliferative quiescence known as G 0 . 18,19,41,42 The state of proliferative quiescence functionally and metabolically differs from other non-proliferative cells. Established examples of translationally regulated mRNAs include those for growth factors and cytokines (FGF-2, IGF II, PDGF, TGFβ, VEGF, IL-15, WNT/β-catenin), protein kinases (MOS, PIM-1), transcription factors (FOS, MYC), enzymes of polyamine biosynthesis (ornithine decarboxylase and ornithine aminotransferase), inhibitors of apoptosis (survivin, Bcl-2, Bcl-X L ) and promoters of cell cycle transit (RAS, Cdk 4, cyclin D1).…”

Section: Translational Control Of the Cell Cyclementioning

confidence: 99%

Translational control of cell fate: From integration of environmental signals to breaching anticancer defense

Bitterman

Polunovsky²

2012

Cell Cycle

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“…Quiescent cells are usually considered less sensitive than proliferating cells to the lethal activity of most anti-cancer drugs (Epifanova et al, 1978;Valeriote and van Putten, 1975). DOX and DAU are less effective against tumor cells in the stationary phase of growth (Drewinko et al, 1981).…”

mentioning

confidence: 99%

Identification of high‐density lipoprotein in serum to determine anti‐cancer efficacy of doxorubicin in HeLa cells

Yung¹,

Bor²

1992

Intl Journal of Cancer

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The cytotoxic effects of doxorubicin (DOX) and daunorubicin (DAU) on HeLa cells cultured under different serum conditions were analyzed by the "nucleophosmin translocation" assay using immunofluorescence. Bright nucleolar fluorescence was observed in untreated cells. A shift from nucleolar to nuclear fluorescence was observed with increasing doses of DOX or DAU, with longer incubation times. A lesser degree of nucleophosmin translocation from nucleoli to nucleoplasm was observed in serum-deprived cells under the same DOX or DAU treatment. These results correlated well with those of cell-growth-reversibility and colony-formation studies, showing decreased inhibitory effects of growth on cells cultured in medium without serum. Furthermore, cells cultured in medium supplemented with the lipoprotein-free serum responded to DOX in a similar way to cells cultured without serum. High-density lipoprotein (HDL) and low-density lipoprotein (LDL) were then added to the lipoprotein-free serum. Cells cultured in medium with the HDL-supplemented, serum showed increased sensitivity to DOX. Inhibition of cell growth and colony formation was observed in such HDL-supplemented cells upon DOX treatment (30 min). LDL, on the other hand, did not show an increase in the anti-cancer response. These results suggested that the variation in response of cells to DOX anti-cancer treatment under different growth conditions may be due to their varied concentrations of HDL. "Nucleophosmin translocation", which is useful for monitoring and ensuring the efficacy of the drug during anti-cancer treatment, provides an improved potential for successful chemotherapy.

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Responses of proliferating and non-proliferating Chinese hamster cells to cytotoxic agents

Cited by 15 publications

References 21 publications

Hypericin-photodynamic therapy inhibits the growth of adult T-cell leukemia cells through induction of apoptosis and suppression of viral transcription

Hypericin-photodynamic therapy inhibits the growth of adult T-cell leukemia cells through induction of apoptosis and suppression of viral transcription

Translational control of cell fate: From integration of environmental signals to breaching anticancer defense

Identification of high‐density lipoprotein in serum to determine anti‐cancer efficacy of doxorubicin in HeLa cells

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