Responses of Raphidocelis subcapitata exposed to Cd and Pb: Mechanisms of toxicity assessed by multiple endpoints

Alho, Lays de Oliveira Gonçalves; Gebara, Renan Castelhano; Paina, Karime de Araujo; Sarmento, Hugo; Melão, Maria da Graça Gama

doi:10.1016/j.ecoenv.2018.11.087

Cited by 50 publications

(21 citation statements)

References 63 publications

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“…Flow‐cytometric analysis of cellular complexity (or granularity) may indicate the accumulation of the metal internalized by the algal cell (Alho et al, 2019), by the formation of either vacuoles or vesicles. This is an algal mechanism to cope with the presence of heavy metals, by reducing or preventing their toxic effects (Juarez et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…This result was probably due to the assay conditions used by the authors, such as the pH they used in the culture medium (pH 6.5), because in the circumneutral solution Cr (III) was reported to be 5-10 times more toxic to green alga than Cr (VI) (Vignati et al, 2010). In general, heavy metal stress induces an increase in ROS in algal cells, such as that reported by Alho et al (2019) and Gebara et al ( 2020) by exposing R. subcapitata to Cd and Pb and to Zn and Al, respectively. In their studies, ROS played a major role in inhibiting algal growth as a result of the metal exposure.…”

Section: Toxicity Of Isolated Metalsmentioning

confidence: 90%

“…The mean values of FL3-H (mean cell Chl a fluorescence), SSC-H (mean cell complexity), and forward-scatter (FSC-H; mean cell size) of the microalgae and the bead population enabled the calculation of mean relative FL3-H, mean relative SSC-H, and mean relative FSC-H, as described in Mansano et al (2017), which were expressed in arbitrary units. The relative ROS assessment followed the procedures described in detail in Alho et al (2019). Data acquisition was done using CellQuest BD software and cytogram analysis using FlowJo V10 software.…”

Section: Flow-cytometric Analysismentioning

confidence: 99%

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Individual and Combined Effects of Manganese and Chromium on a Freshwater Chlorophyceae

Alho

Gebara

Mansano

et al. 2022

Environmental Toxicology and Chemistry

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Manganese (Mn), an essential metal in trace amounts, and chromium (Cr), a nonessential metal to algae, are often found in effluent discharges and may co‐occur in contaminated aquatic environments. Therefore, we investigated the effects of Mn and Cr, and their mixtures, on a freshwater Chlorophyceae, Raphidocelis subcapitata, using a multiple endpoint approach. Regarding the single exposure of metals, Mn was 4 times more toxic (median inhibitory concentration at 72 h [IC5072 h] = 4.02 ± 0.45 µM Mn) than Cr (IC5072 h = 16.42 ± 4.94 µM Cr) for microalgae, considering the effects on the relative growth rate. Moreover, this species was the most sensitive to Mn, according to the species sensitivity distribution curve. Overall, the tested metals did not lead to significant changes in reactive oxygen species production, cellular complexity, and cell relative size but significantly decreased the algal growth and the mean cell chlorophyll a (Chl a) fluorescence at the highest concentrations (3.64–14.56 µM of Mn and 15.36–19.2 µM of Cr). The decreased mean cell Chl a fluorescence indicates an impact on pigment synthesis, which may be related to the observed growth inhibition. In the mixture tests, the reference models concentration addition and independent action were used to analyze the data, and the independent action model was the best fit to describe our results. Therefore, the Mn and Cr mixture was noninteractive, showing additive effects. This is the first study to address the combined toxicity of Mn and Cr regarding freshwater Chlorophyceae. Environ Toxicol Chem 2022;41:1004–1015. © 2022 SETAC

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Section: Resultsmentioning

confidence: 99%

Section: Toxicity Of Isolated Metalsmentioning

confidence: 90%

Section: Flow-cytometric Analysismentioning

confidence: 99%

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Individual and Combined Effects of Manganese and Chromium on a Freshwater Chlorophyceae

Alho

Gebara

Mansano

et al. 2022

Environmental Toxicology and Chemistry

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“…These results indicated that Cd 2+ is much toxic than Pb 2+ to green algae. Similarly, Alho et al [21] revealed that Cd 2+ is greatly more toxic to the alga Raphidocelis subcapitata than Pb 2+ . Li et al…”

Section: Effects Of CD 2+ and Pb 2+ On Photosynthesis Fluorescence Pa...mentioning

confidence: 93%

Effects of Cd<sup>2+</sup> and Pb<sup>2+</sup> on Growth and Photosynthesis of Two Freshwater Algae Species

Dong¹,

Wang²,

Wang³

et al. 2022

Pol. J. Environ. Stud.

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Microalgae are biological indicators of heavy metal pollution. Cadmium (Cd) and lead (Pb) are extremely toxic metals to aquatic organisms. In the present study, single and combined toxicity of Cd 2+ and Pb 2+ to Scenedesmus acutus and Schroederia sp. collected from the famous Xin'an River (Huangshan City) were evaluated. Treatments with 0.5-2.0 mg/L Cd 2+ significantly reduced S. acutus population growth, and treatment with 2.0 mg/L Cd 2+ significantly decreased Schroederia sp. population growth rate, suggesting a higher tolerance of Schroederia sp. than S. acutus to Cd 2+ pollution. In addition, Cd 2+ treatments significantly decreased chlorophyll a, carotene contents, and photosynthetic fluorescent parameters rETR max and I k , demonstrating that the harms on photosynthesis might be the underlying mechanism of Cd 2+ toxicity to algae. Treatments with 5.0-15.0 mg/L Pb 2+ did not significantly affected population growth and photosynthetic pigment content. However, combined exposure to Cd 2+ and Pb 2+ revealed antagonistic effects on both species. Overall, these results provide basic information to the ecological risk assessments of heavy metal pollution in the Xin'an River Basin.

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“…Plant cytology experiments found that, HMs stress not only poses a threat to photosynthetic capacity by reducing leaf chlorophyll content (Huihui et al, 2020), but also causes peroxidation of cell membrane structures as they damage tissues of living plants (Alho, Gebara, Paina, Sarmento, & Melao, 2019). Modern technologies, like Fouriertransform infrared (FTIR) has been shown to be an effective tool to diagnose HM stress at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Two hyperspectral indices for detecting cadmium and lead contamination from arice canopy spectrum

et al. 2020

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Neither Cadmium (Cd) nor lead (Pb) is necessary for crop growth, and they both cause severe soil pollution in many countries. A cross-stress experiment was designed to investigate the feasibility of diagnosing the type and the level of Cd-Pb cross-stress by observing rice canopy hyperspectral images. Two-way analysis of variance and random forest algorithm were employed to select the sensitive indices for Cd-Pb cross-stress diagnosing. Following the exploration of bandwidth expansion from 1 to 20 nm, the final sensitive indices were proposed. We proposed two indices for distinguishing the two heavy metals named: the cadmium stress vegetation index (CSVI) and the lead stress vegetation index (LSVI). The diagnostic accuracies of CSVI distinguishing the four different Cd-stressed levels (0, 2, 5, 8 mg L −1 ) reached 0.85, 0.92, 0.96, and 0.92, respectively, while the precision for four Pb-stressed levels (0, 50,

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Responses of Raphidocelis subcapitata exposed to Cd and Pb: Mechanisms of toxicity assessed by multiple endpoints

Cited by 50 publications

References 63 publications

Individual and Combined Effects of Manganese and Chromium on a Freshwater Chlorophyceae

Individual and Combined Effects of Manganese and Chromium on a Freshwater Chlorophyceae

Effects of Cd<sup>2+</sup> and Pb<sup>2+</sup> on Growth and Photosynthesis of Two Freshwater Algae Species

Two hyperspectral indices for detecting cadmium and lead contamination from arice canopy spectrum

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