Responses of soil enzyme activity and microbial community compositions to nitrogen addition in bulk and microaggregate soil in the temperate steppe of Inner Mongolia

Shi, Yao; Sheng, Lianxi; Wang, Zhongqiang; Zhang, Xinyu; He, Nianpeng; Yu, Qiang

doi:10.1134/s1064229316100124

Cited by 31 publications

(21 citation statements)

References 81 publications

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“…NAG is a chitin-degrading enzyme that is also involved in the degradation of peptidoglycan, which is a component of the bacterial cell wall. LAP can break the N-terminal amino acid of the polypeptide into leucine 33,34 . Amino acids and other nitrogenous compounds are important forms of soluble soil organic carbon 35 ; thus, their activities are closely related to microbial available nutrients.…”

Section: Discussionmentioning

confidence: 99%

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Liu¹,

Yang²,

Yang³

et al. 2019

Sci Rep

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Naphthalene has been widely used to study the role of soil fauna, but its potential non-target effects on soil enzyme activity remain unknown in subalpine forests. We added naphthalene for two years and determined the effect of such additions on the abundance of soil fauna and soil enzyme activities (β-glucosidase, cellobiohydrolase, invertase, peroxidase, polyphenol oxidase, N-acetyl-β-D-glucosaminidase, leucine arylamidase, urease, nitrate reductase and nitrite reductase) in a subalpine forest. Naphthalene could efficiently suppress the individual density and population of soil fauna in situ . The individual density and number of groups were decreased by 72.6–84.8% and 15.0–28.0%, respectively. Naphthalene significantly affected the activities of β-glucosidase, cellobiohydrolase, polyphenol oxidase, N-acetyl-β-D-glucosaminidase, leucine arylamidase and nitrite reductase and the activity increased in the first litter peak of naphthalene addition, and decreased at the later. The activities of β-glucosidase, cellobiohydrolase, polyphenol oxidase, peroxidase, N-acetyl-β-D-glucosaminidase, leucine arylamidase and nitrite reductase showed a negative correlation with the soil microbial PLFAs. Conversely, the activities of invertase, urease and nitrate reductase were positively correlated with the soil microbial PLFAs. Our results suggest that naphthalene is an effective method to reduce soil fauna in subalpine forest. The enzyme activity was influenced by soil fauna and microbial PLFAs.

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Section: Discussionmentioning

confidence: 99%

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Liu¹,

Yang²,

Yang³

et al. 2019

Sci Rep

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“…EC was determined using an electrical conductivity instrument (DDS-307, INESA, Shanghai, China). AN was measured using a flow analyzer (Futura, AMS, Frépillon, France), and the pretreatment was completed using the alkaline hydrolysis and diffusion extraction method [52]. TN was measured on the Kjeldahl apparatus (Kjeltec 8400, FOSS, Hillerød, Denmark).…”

Section: Measurements Of the Characteristics Of Plant Amf And Soilmentioning

confidence: 99%

“…TN was measured on the Kjeldahl apparatus (Kjeltec 8400, FOSS, Hillerød, Denmark). AP was extracted with 0.5 mol·L 1− NaHCO 3 solution (pH 8.5) and was determined using Molybdenum antimony and the scandium colorimetry method on a spectrophotometer (UV5−500, METASH, Shanghai, China) [52]. TP was measured using an automatic discontinuous chemical analyzer (Smartchem 450, AMS, Rome, Italy) after digesting the soil samples with sulfuric acid-perchloric acid.…”

Section: Measurements Of the Characteristics Of Plant Amf And Soilmentioning

confidence: 99%

Interaction Between Root Exudates of the Poisonous Plant Stellera chamaejasme L. and Arbuscular Mycorrhizal Fungi on the Growth of Leymus Chinensis (Trin.) Tzvel

Zhu

Xing

et al. 2020

Microorganisms

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The growth of a large number of poisonous plants is an indicator of grassland degradation. Releasing allelochemicals through root exudates is one of the strategies with which poisonous plants affect neighboring plants in nature. Arbuscular mycorrhizal fungi (AMF) can form a mutualistic symbiosis with most of the higher plants. However, the manner of interaction between root exudates of poisonous plants and AMF on neighboring herbage in grasslands remains poorly understood. Stellera chamaejasme L., a common poisonous plant with approved allelopathy, is widely distributed with the dominant grass of Leymus chinensis in the degradeds of Northern China. In this study, we investigated the addition of S. chamaejasme root exudates (SRE), the inoculation of AMF, and their interaction on the growth and tissue nitrogen contents of L. chinensis, the characteristics of rhizosphere AMF, and soil physicochemical properties. Results showed that SRE had significant effects on ramet number, aboveground biomass, and total nitrogen of L. chinensis in a concentration dependent manner. Additionally, SRE had a significant negative effect on the rate of mycorrhiza infection and spore density of the AMF. Meanwhile, the addition of SRE significantly affected soil pH, electrical conductivity, available nitrogen (AN), available phosphorus (AP), total nitrogen (TN), and total carbon (TC) contents; while neither inoculation of AMF itself nor the interaction of AMF with SRE significantly affected the growth of L. chinensis. The interaction between AMF and SRE dramatically changed the pH, AP, and TC of rhizosphere soil. Therefore, we suggested SRE of S. chamaejasme affected the growth of L. chinensis by altering soil pH and nutrient availability. AMF could change the effect of SRE on soil nutrients and have the potential to regulate the allelopathic effects of S. chamaejasme and the interspecific interaction between the two plant species. We have provided new evidence for the allelopathic mechanism of S. chamaejasme and the regulation effects of AMF on the interspecific relationship between poisonous plants and neighboring plants. Our findings reveal the complex interplay between the root exudates of poisonous plants and rhizosphere AMF in regulating population growth and dynamics of neighboring plants in degraded grassland ecosystems.

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“…CC-BY 4.0 International license perpetuity. It is made available under a preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in The copyright holder for this this version posted November 9, 2020. ; https://doi.org/10.1101/2020.11.09.374033 doi: bioRxiv preprint 4 organisms excluding plant bodies, and which is the most active component of soil organic matter and the most active factor in the soil [17]. Soil microbial biomass is the driving force for the transformation and cycling of soil organic matter and soil nutrients; it is also a reserve for soil nutrients and an important source of nutrients that are available for plant growth, and can therefore be used as an important indicator of soil fertility [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Soil microbial community responses to short-term nitrogen addition in China’s Horqin Sandy Land

Niu

Duan

et al. 2020

Preprint

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Anthropogenic nitrogen (N) addition has increased soil nutrient availability, thereby affecting ecosystem processes and functions in N-limited ecosystems. Long-term N addition decreases plant biodiversity, but the effects of short-term N addition on soil microbial community is poorly understood. The present study examined the impacts of short-term N addition (NH4NO3) on these factors in a sandy grassland and semi-fixed sandy land in the Horqin Sandy Land. We measured the responses of soil microbial biomass C and N; on soil β-1,4-glucosidase (BG) and β-1,4-N-acetylglucosaminidase (NAG) activity; and soil microflora characteristics to N additions gradient with 0 (control), 5 (N5), 10 (N10), and 15 (N15) g N m−2 yr−1. The soil microbial biomass indices, NAG activity, and soil microflora characteristics did not differ significantly among the N levels, and there was no difference at the two sites. The competition for N between plants and soil microbes was not eliminated by short-term N addition due to the low soil nutrient and moisture contents, and the relationships among the original soil microbes did not change. However, N addition increased BG activity in the N5 and N10 additions in the sandy grassland, and in the N5, N10, and N15 additions in the semi-fixed sandy land. This may be due to increased accumulation and fixation of plant litter into soils in response to N addition, leading to increased microbial demand for a C source and increased soil BG activity. Future research should explore the relationships between soil microbial community and N addition at the two sites.

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Responses of soil enzyme activity and microbial community compositions to nitrogen addition in bulk and microaggregate soil in the temperate steppe of Inner Mongolia

Cited by 31 publications

References 81 publications

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Effects of naphthalene on soil fauna abundance and enzyme activity in the subalpine forest of western Sichuan, China

Interaction Between Root Exudates of the Poisonous Plant Stellera chamaejasme L. and Arbuscular Mycorrhizal Fungi on the Growth of Leymus Chinensis (Trin.) Tzvel

Soil microbial community responses to short-term nitrogen addition in China’s Horqin Sandy Land

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