Responses of the alga Pseudokirchneriella subcapitata to long-term exposure to metal stress

Machado, Manuela D.; Lopes, Ana Rita; Soares, Eduardo V.

doi:10.1016/j.jhazmat.2015.04.022

Cited by 74 publications

(23 citation statements)

References 56 publications

(77 reference statements)

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“…The freshwater alga Pseudokirchneriella subcapitata is highly sensitive to heavy metals ; therefore, it is frequently used as a standard organism in toxicological tests . Cd, Co, Cr, Cu, Mn, and Zn had a detrimental effect on cell structure and metabolism of P. subcapitata . Bossuyt and Janssen observed an increase of chlorophyll and carotenoids content, and a decrease of biomass of P. subcapitata exposed to 0.007–1.6 μmol L −1 Cu.…”

Section: Introductionmentioning

confidence: 95%

“…Machado et al reported that Cd, Cr, Cu, and Zn have different effects on growth, cell division, and cell volume of P. subcapitata , suggesting that distinct mechanisms underlie the action of each metal. The 72 h exposure of the alga to 1.9 μmol L −1 Cd, 41 μmol L −1 Cr, 1.3 μmol L −1 Cu, and 2.5 μmol L −1 Zn resulted in a severe perturbation of the cell physiological status by loss of membrane integrity, reduction of chlorophyll a content, and the maximum quantum yield of photosystem II, inhibition of esterase activity, and alteration of mitochondrial membrane potential . Soto et al also reported a significant decrease in chlorophyll a in algal cells exposed to 1.2 μmol L −1 Cu and 1.1 μmol L −1 Zn.…”

Section: Introductionmentioning

confidence: 97%

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Short‐ and Long‐Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata

Machado

Soares

2016

CLEAN Soil Air Water

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Research Article Short-and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitataAlgal cells can be exposed to toxicants for a short-term due to accidental discharges or, more commonly, for a long-term. The present work aimed to assess the ability of Cd, Cr, Cu, and Zn to induce accumulation of reactive oxygen species (ROS) in the alga Pseudokirchneriella subcapitata after a short (6 h) or a long (72 h) exposure time. The relationship between the ROS induction and the content of reduced glutathione (GSH) was also examined. For this purpose, three nominal concentrations of each metal were used corresponding approximately to 72 h-EC 10 and 72 h-EC 50 values and a high concentration (>72 h-EC 90 values). Intracellular ROS accumulation and GSH content were evaluated using a fluorescent-based approach. A long-term (chronic) exposure of algal cells to Cd, Cu, and Zn, at the highest concentrations tested, induced an increase of intracellular ROS and GSH content. The increase of GSH content might be a form of algal cells to redress the imbalance caused by the oxidative stress. However, the increase of GSH was not enough to protect the algal cells against the long-term exposure to oxidative stress. The exposure of algal cells to low or intermediate metals concentrations induced a modification of GSH content; however, no increase of ROS production was detected, which indicates that the toxic symptoms exhibited by algal cells, under these conditions, cannot be attributed to intracellular ROS accumulation.Abbreviations: DCF, 2 0 ,7 0 -dichlorofluorescein; EC 10 , EC 50 , EC 90 , concentration of the toxicant that caused the inhibition of 10%, 50%, or 90% of algal growth; GSH, reduced glutathione; H 2 DCF, 2 0 ,7 0dichlorodihydrofluorescein; H 2 DCFDA, 2 0 ,7 0 -dichlorodihydrofluorescein diacetate; mBCl, monochlorobimane; OECD, organization for economic co-operation and development; ROS, reactive oxygen species; SD, standard deviation. 1578

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Short‐ and Long‐Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata

Machado

Soares

2016

CLEAN Soil Air Water

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“…Devido a esta característica, Machado et al (2015) escolheu a P. subcapitata para estudar a sua resposta a longos períodos de exposição ao estresse de metais. O estudo partiu de dados existentes indicativos de que esta alga verde é amplamente usada em avaliação de risco ecológico, com base nas características de crescimento da mesma.…”

Section: Pseudokirchneriella Subcapitataunclassified

“…O estudo partiu de dados existentes indicativos de que esta alga verde é amplamente usada em avaliação de risco ecológico, com base nas características de crescimento da mesma. Machado et al (2015), por sua vez, quiseram identificar as causas fisiológicas responsáveis por inibir o crescimento das algas expostas a metais. Para todos os metais testados, foi constatada a inibição da atividade de esterase, variando conforme à dose aplicada.…”

Section: Pseudokirchneriella Subcapitataunclassified

Algas como bioindicadores da qualidade da água

Marques¹,

Américo-Pinheiro²

2017

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“…As a rule, at first, there is a nonspecific reaction, typical for any irritations. For most of the cells, there is a change of cell membrane permeability during the action of metal ions [2] and subsequent activation of intracellular systems aimed at the suppression of stress reactions and stabilization of intracellular metabolism [3]. For example, in the presence of calcium ions at the initial stage is an activation of different intracellular systems: protein kinases, phospholipases, protein biosynthesis systems, phosphodiesterase, cyclic nucleotides, adenylatecyclase, a contractile apparatus of the cell, etc.…”

Section: Introductionmentioning

confidence: 99%

Biosensors Based on Micro-algae for Ecological Monitoring of the Aquatic Environment

Voznesenskiy¹,

Popik²,

Gamayunov³

et al. 2016

Algae - Organisms for Imminent Biotechnology

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This chapter is devoted to research possibilities of using micro-algae as sensor elements for biological sensors of aquatic pollutants. The characteristics of the fluorescence of microalgae pigments caused by laser light, called laser-induced fluorescence LIF , were used as informative parameters. It is shown that the functioning of biological sensors is determined by the consistency and concentration of micro-algal cells as well as their internal state, which depends on the temperature, illumination, and chemical composition of the water. The results indicate a significant dependence of the LIF intensities of micro-algae from exposure of heavy metal ions, temperature, and illumination. In text is demonstrated specificity of the reaction of various micro-algae, belonging to different phylum. This fact can be used for identify the phylum of micro-algae in situ, and for the creation the biosensors of ecological monitoring aquatic environment.Keywords: laser-induced fluorescence, micro algae phylum, ions of heavy metals, depending on the lighting and temperature, biosensors . IntroductionMonitoring of aquatic environments requires obtained information in real time, long before the appearance of visible signs of pollution, far exceeding the norms of maximum permissible concentrations MPC . In recent years, organisms, which have high sensitivity to adverse factors, are widely studied as bio-indicators for water monitoring. Single-celled organisms including Unicellular microorganisms violation of life appears to change their functioning reversibly or irreversibly. In the latter case usually occur damage cells, typically leading to its destruction. The initial, specific impact of the damaging environmental factor is aimed at very specific molecular structure of the cells. The composition of unicellular organisms mainly includes nucleic acids, proteins, lipids, and polysaccharides. "ll these components may be the target for of the damaging action of factors of the environment surrounding the cell. Violation of the functions of these structures causes a cascade of events that ends with response of the cell as a whole system. Thus, it is possible to distinguish several stages cellular response to external adverse impact. "s a rule, at first, there is a nonspecific reaction, typical for any irritations. For most of the cells, there is a change of cell membrane permeability during the action of metal ions [ ] and subsequent activation of intracellular systems aimed at the suppression of stress reactions and stabilization of intracellular metabolism [ ]. For example, in the presence of calcium ions at the initial stage is an activation of different intracellular systems protein kinases, phospholipases, protein biosynthesis systems, phosphodiesterase, cyclic nucleotides, adenylatecyclase, a contractile apparatus of the cell, etc. This is the first, reversible stage when the cell tries to compensate the effect of damaging factors. When the damaging factor is greater or more prolonged exposure, violation of cellular fu...

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Responses of the alga Pseudokirchneriella subcapitata to long-term exposure to metal stress

Cited by 74 publications

References 56 publications

Short‐ and Long‐Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata

Short‐ and Long‐Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata

Algas como bioindicadores da qualidade da água

Biosensors Based on Micro-algae for Ecological Monitoring of the Aquatic Environment

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