Responses of the Mediterranean seagrass Cymodocea nodosa to combined temperature and salinity stress at the ionomic, transcriptomic, ultrastructural and photosynthetic levels

Tsioli, Soultana; Koutalianou, Maria; Gkafas, Georgios A.; Exadactylos, Athanasios; Papathanasiou, V.; Katsaros, Christos; Orfanidis, Sotiris; Küpper, Frithjof C.

doi:10.1016/j.marenvres.2021.105512

Cited by 17 publications

(7 citation statements)

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“…In this sense, Cymodocea nodosa (Ucria) Ascherson 1870, one of the five most widespread Mediterranean seagrass species [28] and belonging to the taxonomic families required for the determination of a Species Sensitivity Distribution (SSD) (EU Technical Guidance Document of the European Union (EU), 2003) [29], can act as one BPA "bioindicator" organism [25]. C. nodosa has the ability to act as a "bioindicator" organism of many anthropogenic-derived pressures [30], such as metals or metallic nanoparticles [31][32][33][34][35] and some cellular and physiological 'markers' were determined, for studying the impact of these pollutants [25,[31][32][33][34][35]. Moreover, biotic indexes, in C. nodosa, for the ecological status assessment of coastal and transitional waters have also been established [36,37].…”

Section: Introductionmentioning

confidence: 99%

The Enzymatic and Non-Enzymatic Antioxidant System Response of the Seagrass Cymodocea nodosa to Bisphenol-A Toxicity

Malea

Kokkinidi

Kevrekidou

et al. 2022

IJMS

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The effects of environmentally relevant bisphenol A (BPA) concentrations (0.3, 1 and 3 μg L−1) were tested at 2, 4, 6 and 8 days, on intermediate leaves, of the seagrass Cymodocea nodosa. Hydrogen peroxide (H2O2) production, lipid peroxidation, protein, phenolic content and antioxidant enzyme activities were investigated. Increased H2O2 formation was detected even at the lowest BPA treatments from the beginning of the experiment and both the enzymatic and non-enzymatic antioxidant defense mechanisms were activated upon application of BPA. Elevated H2O2 levels that were detected as a response to increasing BPA concentrations and incubation time, led to the decrease of protein content on the 4th day even at the two lower BPA concentrations, and to the increase of the lipid peroxidation at the highest concentration. However, on the 6th day of BPA exposure, protein content did not differ from the control, indicating the ability of both the enzymatic and non-enzymatic mechanisms (such as superoxide dismutase (SOD) and phenolics) to counteract the BPA-derived oxidative stress. The early response of the protein content determined that the Low Effect Concentration (LOEC) of BPA is 0.3 μg L−1 and that the protein content meets the requirements to be considered as a possible early warning “biomarker” for C. nodosa against BPA toxicity.

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Section: Introductionmentioning

confidence: 99%

The Enzymatic and Non-Enzymatic Antioxidant System Response of the Seagrass Cymodocea nodosa to Bisphenol-A Toxicity

Malea

Kokkinidi

Kevrekidou

et al. 2022

IJMS

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“…However, further detailed studies are warranted to assess the ecophysiological consequences of these discharges into the marine environment of Kuwait (Al-Said et al 2017). A recent study on the Mediterranean seagrass Cymodocea nodosa (Tsioli et al 2022) provided details of cellular and molecular responses to temperature and salinity stress, as well as the interactions between both stressorsarguably, this approach should also be applied to a representative subset of seaweed species explored in the present study, ideally including both tolerant and sensitive species.…”

Section: Salinity and Temperature Tolerance Treatmentsmentioning

confidence: 99%

Ecophysiology of Kuwaiti macroalgae with special emphasis on temperature and salinity tolerance related to the conditions at desalination plant outfalls

Hajiya Hasan,

Al-Bader,

Woodward

et al. 2023

Botanica Marina

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Brine discharged from seawater desalination plants impacts marine life by exposure to increased salinity and, in some cases, temperature. However, the responses of individual species to such stress remains poorly known yet their understanding is essential for assessing and predicting the impacts of seawater desalination plants. In this study, unialgal cultures obtained by the germling emergence method of 34 taxa representative of the Rhodophyta, Chlorophyta and Phaeophyceae seaweeds in the Arabian Gulf, and isolated from the vicinity of two large desalination plants in Kuwait, were subjected to increased temperature and salinity under experimental conditions in the laboratory. The dataset is complemented by measurements of seawater temperature and salinity obtained at increasing distances from the outfalls of desalination plants and along the Kuwaiti coastline including from two pristine areas, Boubiyan and Fintas. Chlorophyta, especially Ulva spp., and Phaeophyceae displayed remarkable tolerance against hypersaline and thermal stress, suggesting that this group can cope better with adverse environmental conditions. Members of the Rhodophyta were considerably more sensitive to temperature increases.

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“…Interactions among stressors can push seagrass populations above their functional threshold, where range contractions and extinctions are likely to occur (York et al, 2013;Lefcheck et al, 2017;Kendrick et al, 2019). Gene expression studies have provided useful information on climate stressors in isolation, but only recently have stressors been tested in combination (Zhang and Sonnewald, 2017;Tsioli et al, 2022;Santillań-Sarmiento et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Comparative gene co-expression networks show enrichment of brassinosteroid and vitamin B processes in a seagrass under simulated ocean warming and extreme climatic events

Booth,

Sinclair,

Jung

et al. 2024

Front. Plant Sci.

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IntroductionOcean warming combined with extreme climatic events, such as marine heatwaves and flash flooding events, threaten seagrasses globally. How seagrasses cope with these challenges is uncertain, particularly for range-edge populations of species such as Posidonia australis in Shark Bay, Western Australia. Analyzing gene expression while manipulating multiple stressors provides insight into the genetic response and resilience of seagrasses to climate change. We conducted a gene expression study on a polyploid clone of P. australis during an 18-week mesocosm experiment to assess the responses to single and combined future climate change-associated stressors. MethodsPlants were exposed to (1) future ocean warming temperature (baseline +1.5°C) followed by a simulated marine heat wave (baseline +5.5°C), (2) light deprivation simulating observed marine heatwave driven turbidity (95% shade) at baseline temperatures, or (3) both stressors simultaneously. Basal leaf meristems were sampled for gene expression analysis using RNA-seq at four time points during the experiment. Weighted gene co-expression network analysis, GO term enrichment, and KEGG pathway enrichment analyses were used to identify stress responses. ResultsShaded plants showed specific gene enrichment for shade avoidance (programmed cell death) after three weeks of stress, and before any heated tanks showed a specific heat response. Shaded plants were positively correlated with programmed cell death and stress-related processes at the end of the experiment. Once ocean warming temperatures (+1.5°C) were in effect, gene enrichment for heat stress (e.g., ROS scavenging and polyamine metabolism) was present. Vitamin B processes, RNA polymerase II processes. and light-related meristematic phase changes were expressed with the addition of simulated MHW. Heated plants showed meristematic growth signatures as well as trehalose and salicylic acid metabolism. Brassinosteroid-related processes were significantly enriched in all stressor treatments at all time points, except for the isolated heat-stressed plants three weeks after stressor initiation. DiscussionGene expression responses to the interaction between heat waves and turbidity-induced light reduction support the observed geographical scale mortality in seagrasses observed for P. australis in Shark Bay, suggesting that even this giant polyploid clone will be negatively impacted by more extreme climate change projections.

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Responses of the Mediterranean seagrass Cymodocea nodosa to combined temperature and salinity stress at the ionomic, transcriptomic, ultrastructural and photosynthetic levels

Cited by 17 publications

References 93 publications

The Enzymatic and Non-Enzymatic Antioxidant System Response of the Seagrass Cymodocea nodosa to Bisphenol-A Toxicity

The Enzymatic and Non-Enzymatic Antioxidant System Response of the Seagrass Cymodocea nodosa to Bisphenol-A Toxicity

Ecophysiology of Kuwaiti macroalgae with special emphasis on temperature and salinity tolerance related to the conditions at desalination plant outfalls

Comparative gene co-expression networks show enrichment of brassinosteroid and vitamin B processes in a seagrass under simulated ocean warming and extreme climatic events

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