Responses of the Mushroom Pleurotus ostreatus under Different CO2 Concentration by Comparative Proteomic Analyses

Abstract

Background: Pleurotus ostreatus is a popular edible mushroom in East Asian markets. Research on the responses of P. ostreatus under different carbon dioxide concentrations is limited. Methods: Label-free LC-MS/MS quantitative proteomics analysis technique was adopted to obtain the protein expression profiles of P. ostreatus fruiting body pileus collected under different carbon dioxide concentrations. The Pearson correlation coefficient analysis and principal component analysis were performed to reveal the correlation among samples. The differentially expressed proteins (DEPs) were organized. Gene ontology analysis was performed to divide the DEPs into different metabolic processes and pathways. Results: The expansion of stipes was inhibited in the high CO2 group compared with that in the low CO2 group. There were 415 DEPs (131 up- and 284 down-regulated) in P. ostreatus PH11 treated with 1% CO2 concentration compared with P. ostreatus under atmospheric conditions. Proteins related to hydrolase activity, including several amidohydrolases and cell wall synthesis proteins, were highly expressed under high CO2 concentration. Most of the kinases and elongation factors were significantly down-regulated under high CO2 concentration. The results suggest that the metabolic regulation and development processes were inhibited under high CO2 concentrations. In addition, the sexual differentiation process protein Isp4 was inhibited under high CO2 concentrations, indicating that the sexual reproductive process was also inhibited under high CO2 concentrations, which is inconsistent with the small fruiting body pileus under high CO2 concentrations. Conclusions: This research reports the proteome analysis of commercially relevant edible fungi P. ostreatus under different carbon dioxide concentrations. This study deepens our understanding of the mechanism for CO2-induced morphological change in the P. ostreatus fruiting body, which will facilitate the artificial cultivation of edible mushrooms.

Comparative Proteomic Analysis Reveals Candidate Pathways Related to the Effect of Different Light Qualities on the Development of Mycelium and Fruiting Body of Pleurotus ostreatus

“…Furthermore, the fruiting bodies of P. ostreatus PH11 were cultured in cultivation bags filled with a previously described cultivation substrate (400 g) . After sterilization at 121 °C for 2 h and cooling at room temperature in an aseptic environment, the cultivation bags were inoculated with 2% P.…”

“…Specifically, proteomic analysis has been successfully applied in a few studies of P. ostreatus, including investigations of factors, such as CO 2 concentration, primordial differentiation, and heat stress. − Therefore, it is necessary to conduct proteomic analysis on the mycelia and fruiting bodies of P. ostreatus.…”

Complete genome sequences and comparative secretomic analysis for the industrially cultivated edible mushroom Lyophyllum decastes reveals insights on evolution and lignocellulose degradation potential

“…Protein pellets were re-dissolved in 4% SDS buffer (in 100 mM Tris–HCl buffer, pH 7.6) and digested using trypsin with STrap methods as described by Zougman et al (2014) . LC-MS analysis was performed in DDA mode as in our previous description without changing ( Lin et al, 2022 ), and the chromatographic time is 90 min. Three biological replicates were performed for each group.…”

“…With genomic information, more genes related to important physiological characteristics and key agricultural traits could be identified (Wang et al, 2018(Wang et al, , 2021a. Besides, the genome could promote the proteome and transcriptome research of mushrooms (Lin et al, 2022).…”

Proteomic Analysis of Pleurotus ostreatus Grown on Glucose and Xylose Mixtures in Submerged Fermentation Provides Insights into Differentiated Mycelial Composition

“…Concerning fruiting body production, proteomic analysis contributes to understanding the primordium differentiation in the early development of the fruiting body and the role of Ca 2+ and highlights the differences between the Cap and Stipe development [21]. Also, the mechanism of CO2-induced morphological change in P. ostreatus fruiting body has been proposed [22]. Finally, a molecularlevel explanation of the genomic instability of subcultures of P. ostreatus and an approach to monitor strain degeneration in industrial cultivation has already been proposed with the aid of proteomic analysis [23].…”