Responses of the Plasma Membrane to Cold Acclimation and Freezing Stress

Yoshida, Sbizuo; Uemura, Matsuo

doi:10.1007/978-3-642-74522-5_13

Cited by 26 publications

(30 citation statements)

References 48 publications

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“…In addition, there is not always strict correlation between freezing tolerance and membrane fluidity, and when an increase in membrane fluidity is observed, it does not necessarily correspond to changes in lipid composition. It seems that there is a general pattern of an increase in lipid to protein ratio with increasing freezing tolerance (35). Indeed, cold acclimation has been shown to induce many biochemical changes with-in plant membranes, and we do not know to what extent these individual components alter the cryobehavior of the membranes.…”

Section: Lateral Diffusion Of Fluorescent Probes In Vacuolar Membranementioning

confidence: 96%

“…The major problem in these correlation studies lies in the fact that the lipid fractions investigated were mostly extracted from whole cells or from microsomal fractions that include a variety of cellular membranes (35). Until recently, this has been primarily due to the lack of methods to isolate sufficient quantities of pure specific membranes from plant tissues.…”

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confidence: 99%

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Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Leborgne¹,

Dupou-Cézanne²,

Teulières³

et al. 1992

Plant Physiol.

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Two cell lines of Eucalyptus gunnii have been shown to keep their differential frost tolerance at the cellular level after longterm culture. They have been used to investigate the fluidity of specific cell membranes in relation with frost tolerance. Protoplasts and isolated vacuoles were obtained from both cell lines. In addition, purified plasma membrane and tonoplast (the vacuolar membrane) were separated from a crude microsomal fraction through free-flow electrophoresis. The lateral and rotational mobilities of lipids in these different membranes were studied by two biophysical techniques: fluorescence recovery after photobleaching (FRAP) and fluorescence polarization. After labeling the vacuoles isolated from the frost-sensitive cells with 1-oleoyl-2-(7-nitro-2,1,3-benzoxadiazol-4-yl)aminocaproyl phosphatidylcholine, a single mobile component was observed with a diffusion coefficient of 2.4 x 10-9 cm2 s-i and a mobile fraction close to 100% at a temperature of 23°C. When using isolated vacuoles from the frost tolerant line, a higher lateral diffusion of tonoplast lipids was found with a diffusion coefficient of 3.2 x 10'9 cm2 s-1, still with a mobile fraction close to 100%. No convincing data were obtained when performing fluorescence recovery after photobleaching experiments on protoplasts. Fluorescence polarization experiments confirmed the differential behavior of the two cell lines for tonoplast and also for plasma membrane. In addition, they showed that intrinsically tonoplast exhibited a higher fluidity than plasma membrane. Our results provide the first information on the fluidity of tonoplast and on the compared properties of two important plant membranestonoplast and plasma membrane-through the use of two complementary biophysical approaches. In addition, they suggest there is a correlation between membrane fluidity and cold tolerance. The potential interest of plant vacuole as a natural model system in membrane studies is emphasized.

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Section: Lateral Diffusion Of Fluorescent Probes In Vacuolar Membranementioning

confidence: 96%

mentioning

confidence: 99%

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Leborgne¹,

Dupou-Cézanne²,

Teulières³

et al. 1992

Plant Physiol.

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“…Needles of acclimated seedlings of Pinus sylvestris had higher phospholipid content than non-acclimated ones [16]. These investigations have been considerably extended by other studies [17][18][19]. These studies observed a clear relationship between the process of acclimation and the concentrations of phospholipids, such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE).…”

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confidence: 61%

Differential effects of spring reacclimation and deacclimation on cell membranes of Norway spruce seedlings

Pukacki

Kamińska-Rożek

2013

Acta Soc Bot Pol

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Two-year-old seedlings of Norway spruce (Picea abies) during spring deacclimation were subjected to controlled reacclimation by exposure to low temperatures of 4/−3°C (day/night) in a cold room. The highest increase in freezing tolerance (by 7°C) was observed after 12 d of low temperature exposure, when shoot water potential (Ψ_{w shoot}) decreased to 0.64 MPa. The process of reacclimation was accompanied by an increase in the phospholipid content of needle cell membranes. This increase applied to total (PL) and individual phospholipids: phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidic acid (PA). After being exposed to the low temperature for 18 d, the seedlings were moved into the open air. This caused deacclimation, with an increase in Ψ_{w shoot} to −0.36 MPa and a decrease in the total phospholipid content and freezing tolerance of the needles. Significant correlations were observed between freezing tolerance, the membrane permeability (MP) of the needles and the phospholipid content, Ψ_{w shoot} and water content of the needles. The results show that during spring deacclimation, Norway spruce seedlings can be subjected to reacclimation, which is reflected in the phospholipid content, the biophysical changes of the membranes, and the freezing tolerance of the seedlings. During both spring deacclimation and reacclimation, water content in the needles plays a critical role in the cold tolerance of spruce seedlings

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“…6) It is generally accepted that freezing injury of plant cells probably results from dysfunction of the cellular membrane induced by structural changes during the freezing process. [7][8][9] In the case of freezing-induced structural changes in the plasma membranes, differential hydration characteristics of membrane components first cause the exclusion of membrane proteins from the regions of the lipid bilayer that are in close proximity to plasma membranes and endomembranes during extracellular freezing. Upon further freezing, dehydration and deformation of the cells induced by severe extracellular freezing promote the demixing of membrane lipids due to lyotropic properties of the lipids, resulting in fusion of plasma membranes with endomembranes.…”

mentioning

confidence: 99%

“…In recent years, many studies have focused on the effects of compositional changes in plasma membrane lipids on the freezing tolerance of plant cells. 15) However, there have been few studies on protein components of plasma membranes that are related to freezing tolerance 9,16,17) and freezing injury. 18) Uemura and Yoshida (1986) studied compositional changes in plasma membranes prepared from coldacclimated tubers of Helianthus tuberosus L. (Jerusalem artichoke) that were subjected to in vivo freezing.…”

mentioning

confidence: 99%

Frost-susceptible Protein in Plasma Membranes in Tubers ofHelianthus tuberosusL.

Arakawa¹,

Hanazaki²,

Yoshida³

2004

Bioscience, Biotechnology, and Biochemistry

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When plasma membranes were prepared from tubers of Helianthus tuberosus L. (Jerusalem artichoke) frozen at a sublethal temperature (À10 C), the levels of some plasma membrane proteins, named frost-susceptible proteins (FSPs), decreased [Uemura, M., et al., Plant Physiol., 80, 187-195 (1986)]. The aim of this study was to characterize the response of FSP120, which is named FSP-3 in a previous report, to freezing treatment by immunoblotting. Levels of FSP120 in the plasma membranes of tubers decreased after sublethal freezing, whereas no degraded products were detected in the microsomes or the soluble fraction. The amount of FSP120 in the crude extract of frozen tubers remained at a comparable level to that of the unfrozen tubers. These results suggest that FSP120 might be released from plasma membranes during freezing treatment of the tubers of Jerusalem artichoke.

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Responses of the Plasma Membrane to Cold Acclimation and Freezing Stress

Cited by 26 publications

References 48 publications

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Differential effects of spring reacclimation and deacclimation on cell membranes of Norway spruce seedlings

Frost-susceptible Protein in Plasma Membranes in Tubers ofHelianthus tuberosusL.

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