Responses of the plasma membrane to low temperatures

Uemura, Matsuo; Tominaga, Yoko; Nakagawara, Chihaya; Shigematsu, Satomi; Minami, Anzu; Kawamura, Yukio

doi:10.1111/j.1399-3054.2005.00594.x

Cited by 218 publications

(175 citation statements)

References 35 publications

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“…This may explain their higher cold sensitivity, considering the role of lipid unsaturation in the maintenance of cell membrane stability (Uemura et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Cruz

Golombieski

Bazana

et al. 2010

Braz. J. Plant Physiol.

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Rice is a tropical plant, so cold temperature may be detrimental to its development, depending on the genotype and environmental conditions. Degree of lipid unsaturation has been related to cold tolerance due to its effect on membrane stability. So, the aim of this study was to characterize the fatty acid composition and its alterations due to cold temperature in rice genotypes of diversified origin. Forty-four rice genotypes at the V 4 stage were submitted to two temperature conditions: 10°C and 28°C for two days and after this they had their leaves collected for lipid extraction and quantification. Control plants were allowed to regrow until presenting four leaves fully expanded and then were subjected to 10°C for ten days for cold tolerance evaluation. Plant survival was measured seven days after recovery at 28°C and the genotypes were grouped in three cold tolerance classes: tolerant, intermediate and sensitive. These classes differed for total saturated and unsaturated fatty acids only under the cold temperature treatment. Further analysis of the more abundant fatty acids: linoleic, linolenic and palmitic, showed that the two last ones differed between tolerant and sensitive genotypes. Linolenic acid increased after cold exposure in cold tolerant genotypes while palmitic acid decreased, and an opposite behavior was found in the cold sensitive genotypes. These evidences indicate that these fatty acids are potential molecular markers useful for breeding programs as well as for future basic studies on cold tolerance in rice.

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“…This may explain their higher cold sensitivity, considering the role of lipid unsaturation in the maintenance of cell membrane stability (Uemura et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Cold acclimation involves altered gene expression that affects membrane composition and accumulation of compatible solutes (Uemura et al, 2006). This is possible through the action of specific enzymes which are capable of altering the level of lipidic unsaturation of membranes.…”

Section: Introductionmentioning

confidence: 99%

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Cruz

Golombieski

Bazana

et al. 2010

Braz. J. Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Cell Organization and Membranes are Remodeled after Cold Acclimation-The increased vesicularization of the C. reinhardtii cells observed during cold stress exposure can be an indicator of progressive membrane damage, since cell vascularization has been reported as reporter under other stress situations (87). Furthermore the rigidification of the membranes has been proposed as one of the first steps in cold stress signaling in Arabidopsis thaliana (5).…”

Section: Discussionmentioning

confidence: 99%

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Valledor

Furuhashi

Hanak

et al. 2013

Molecular & Cellular Proteomics

130

131

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Chlamydomonas reinhardtii is one of the most important model organisms nowadays phylogenetically situated between higher plants and animals (Merchant et al. 2007). Stress adaptation of this unicellular model algae is in the focus because of its relevance to biomass and biofuel production. Here, we have studied cold stress adaptation of C. reinhardtii hitherto not described for this algae whereas intensively studied in higher plants. Toward this goal, high throughput mass spectrometry was employed to integrate proteome, metabolome, physiological and cell-morphological changes during a time-course from 0 to 120 h. These data were complemented with RT-qPCR for target genes involved in central metabolism, signaling, and lipid biosynthesis. Using this approach dynamics in central metabolism were linked to cold-stress dependent sugar and autophagy pathways as well as novel genes in C. reinhardtii such as CKIN1, CKIN2 and a hitherto functionally not annotated protein named CKIN3. Cold stress affected extensively the physiology and the organization of the cell. Gluconeogenesis and starch biosynthesis pathways are activated leading to a pronounced starch and sugar accumulation. Quantitative lipid profiles indicate a sharp decrease in the lipophilic fraction and an increase in polyunsaturated fatty acids suggesting this as a mechanism of maintaining membrane fluidity. The proteome is completely remodeled during cold stress: specific candidates of the ribosome and the spliceosome indicate altered biosynthesis and degradation of proteins important for adaptation to low temperatures. Specific proteasome degradation may be mediated by the observed cold-specific changes in the ubiquitinylation system. Sparse partial least squares regression analysis was applied for protein correlation network analysis using proteins as predictors and Fv/Fm, FW, total lipids, and starch as responses. We applied also Granger causality analysis and revealed correlations between proteins and metabolites otherwise not detectable. Twenty percent of the proteins responsive to cold are uncharacterized proteins. This presents a considerable resource for new discoveries in cold stress biology in alga and plants. Molecular &

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“…In agreement with this notion it was previously shown that dramatic molecular and cellular rearrangements occur in response to low temperature stress or pathogen attack. In the case of cold stress, the cell membrane undergoes a series of changes in both lipid and protein composition such that the rigidity of the plasma membrane is elevated (Uemura et al 2006). Changes in cell wall structures were also observed during plant cell cold acclimation (Weiser et al 1990;Yamada et al 2002;Wei et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Cold responsive EARLI1 type HyPRPs improve freezing survival of yeast cells and form higher order complexes in plants

Schläppi

2007

Planta

104

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Plants have large families of proteins sharing a conserved eightcysteine-motif (8CM) domain. The biological functions of these proteins are largely unknown. EARLI1 is a cold responsive Arabidopsis gene that encodes a hybrid proline-rich protein (HyPRP) with a three-domain architecture: a putative signal peptide at the N-terminus, a proline-rich domain (PRD) in the middle, and an 8CM domain at the C-terminus. We report here that yeast cells expressing different EARLI1 genes had significantly higher rates of freezing survival than empty-vector transformed controls. Arabidopsis plants with knocked down EARLI1 genes had an increased tendency for freezinginduced cellular damage. EARLI1-GFP Fluorescence in transgenic plants and NOT THE PUBLISHED VERSION; this is the author's final, peer-reviewed manuscript. The published version may be accessed by following the link in the citation at the bottom of the page.Planta, Vol. 227, No. 1 (December 2007): pg. 233-243. DOI. This article is © Springer and permission has been granted for this version to appear in e-Publications@Marquette. Springer does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from Springer.2 immunoblot analyses using protoplasts suggested cell wall localization for EARLI1 proteins. Immunoblot analyses showed that EARLI1 proteins form higher order complexes in plants, and that the PRD is a soluble and the 8CM an insoluble protein domain. We propose that EARLI1 proteins have a bimodular architecture in which the PRD may interact with the cell wall and the 8CM domain with the plasma membrane to protect the cells during freezing stress.

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Responses of the plasma membrane to low temperatures

Cited by 218 publications

References 35 publications

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Alterations in fatty acid composition due to cold exposure at the vegetative stage in rice

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Cold responsive EARLI1 type HyPRPs improve freezing survival of yeast cells and form higher order complexes in plants

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