Responses of the sheep blowflies Lucilia sericata and Lcuprina to odour and the development of semiochemical baits

Ashworth, Jeremy R.; Wall, Richard

doi:10.1111/j.1365-2915.1994.tb00093.x

Cited by 132 publications

(79 citation statements)

References 54 publications

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“…Colonization attempts when volatiles were above a threshold resulted in reduced survival of deposited eggs, while the opposite was observed for cohorts deposited with eggs emitting volatile concentrations below threshold levels (Lam et al 2007). The same has been determined for other species including blow flies (Ashworth and Wall, 1994; Vass black soldier flies prefers the sun is shining place to oviposit (Fig. 1).…”

Section: Measurement Of Oviposition Ratessupporting

confidence: 68%

Oviposition site preference in Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae), in Artificial Rearing System

Park¹,

Kim²,

Kim³

et al. 2016

International Journal of Industrial Entomology

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In natural conditions, the black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperate regions, and is active in Korea from May through October. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. This species colonizes and oviposits in a wide variety of decomposing vegetable and animal matter. For mass rearing of black soldier flies, the egg deposition methods are dependent on sunlight and oviposition sites. In this study, we investigated the substrates and oviposition sites preferred by black soldier fly in artificial rearing system. Our results showed that as black soldier flies prefer mating under sunlight, they prefer ovipositing at sites where adequate sunlight and food substrate are available. Further detailed research is required to develop methods for artificially rearing black soldier fly throughout the year in Korea. IntroductionThe black soldier fly, Hermetia illucens (L.), is distributed throughout the tropics and warm temperate regions (James, 1935;McCallan, 1974). This insect is mainly found in the vicinity of and in cattle sheds, manure sheds, living waste dump grounds, and food waste dump grounds (Kim, 1997). Larvae of black soldier fly decompose organic material efficiently, and therefore, have been used to transform organic waste matter to valuable fertilizer. Also, black Soldier fly prepupae can be used as a feed for a variety of animals, including fish (Bondari and Sheppard, 1981) and swine (Newton et al., 1977). Prepupae, when dried, have an estimated value comparable to menhaden fish meal. If used live, as a specialty feed, or marketed to exploit its other unique qualities (i.e., essential fatty acids and chitin), the value of the product might be relatively higher (Sheppard et al., 1994). Females of black soldier fly mate once with one oviposition event in their lifetime, and mated females selectively oviposit 320~620 eggs in dry crevices near a moist food source approximately 2 d after successful copulation (Tomberlin et al., 2002). Therefore, developmental studies, including mating behaviors, were conducted with a goal of maintaining a selfsustaining colony for year-round waste conversion (Booth and Sheppard 1984, Tomberlin andSheppard, 2002). The study of oviposition is essential for developing methods for effective egg collection and year-round mass maintenance of black soldier fly. 54-58 (2016) clutches deposited daily. To make this measurement, a plastic pot (25 cm in diameter × 10 cm in depth)containing 1 kg of 50% food waste and 50% calf feeds was placed in the center of the cage. Individuals oviposited in the holes (5 mm in diameter × 7 mm in depth) of two floral foam square bars (egg collecting units) that were approximately 3 cm above the moist media.The floral foam square bars were replaced daily, and oviposited eggs were removed out of the floral foams. The numbers and weights of egg clutches were recorded. The floral foams were kept under direct sunlight, and the others were placed...

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Section: Measurement Of Oviposition Ratessupporting

confidence: 68%

Oviposition site preference in Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae), in Artificial Rearing System

Park¹,

Kim²,

Kim³

et al. 2016

International Journal of Industrial Entomology

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“…Both species engage in myiasis, larval infestation of animal tissues (Ashworth and Wall 1994), which causes more than US$150 million of annual economic loss to the wool industry in Australia alone (Evans and Karlsson 2003). This behavior has beneficial uses though, as certain L. sericata strains (LB-01) are useful in maggot debridement therapy (MDT) (Mumcuoglu 2001;Sherman 2009).…”

Section: Introductionmentioning

confidence: 99%

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

Singh

Crippen

Zheng

et al. 2014

Appl Microbiol Biotechnol

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Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.

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“…A critical phase in the life cycle of these species is host location. The attraction of blowflies to their hosts is not a unitary process, but involves a broad range of behaviors including activation, orientation, and landing behavior culminating in oviposition (Ashworth and Wall 1994). During the whole process, olfaction has a considerable influence on regulating these behaviors ( Barton Browne 1960;Wall and Warnes 1994;LéBlanc and Logan 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression pattern of an odorant receptor from the myiasis-causing blowfly, Lucilia sericata (Diptera: Calliphoridae)

et al. 2011

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The blowfly Lucilia sericata (Diptera: Calliphoridae) is a facultative ectoparasite that causes myiasis in both man and animals, leading to serious human health problems and economic losses in the livestock industry. Like other insects, olfaction of this species plays an important role in host location and is presumably mediated by a seven transmembrane receptor family. Here, we isolate and characterize LserOR1, which is the first candidate member of the odorant receptor gene family from L. sericata. LserOR1 displayed high amino acid conservation with previously identified Or83b orthologs from different insect species. The transcripts of LserOR1 were detected in the major olfactory organs including the antennae and maxillary palps, as well as in traditionally non-olfactory tissues such as the legs and female ovipositors. In developmental studies, a quantitative real-time PCR assay was developed and validated for determining the relative expression levels of LserOR1 during several stages. In contrast to its extremely high expression in the adult stage, LserOR1 expression was at the lowest level during the egg stage, and then increased to a peak through the first two larval stages before declining in the third-instar stage. These results suggest that a broadly expressed LserOR1 receptor is likely to be essential for olfactory sensory processes throughout the lifetime of L. sericata. The present study provides the information that may aid in the development of novel blowfly repellents using olfactory proteins as molecular targets.

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Responses of the sheep blowflies Lucilia sericata and Lcuprina to odour and the development of semiochemical baits

Cited by 132 publications

References 54 publications

Oviposition site preference in Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae), in Artificial Rearing System

Oviposition site preference in Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae), in Artificial Rearing System

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

Molecular characterization and expression pattern of an odorant receptor from the myiasis-causing blowfly, Lucilia sericata (Diptera: Calliphoridae)

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