Abstract:The aim of the work is to evaluate the influence of physical activity character on concentration of macro-and microelements in hair of physically active students. 127 students of I-IV year of Physical Education program at Lithuanian University of Educational Sciences participated in the research, aged from 20 to 24. They were split into three groups. The first (I) group (n = 48) consisted of endurance sport athletes (skiers, biathlon athletes, middle and long distance runners, rowers and cyclists). The second group (II) (n = 61) was made up of the basketball, football, handball and volleyball players. The third group (III) consisted of combat sports (wrestling and boxing) athletes (n = 18). For establishing the concentration of macro-and microelements in hair, x-ray fluorescence method was applied, using CEP-1 ELVAX (MP.MN 3730-2011) device. The results showed that the hair of the students of Physical Education program, practicing endurance sports possessed more calcium, copper, manganese in comparison to the athletes practicing other sports. Concentration of the main elements-zinc, iron, selenium and chromium in the hair of combat sports representatives was statistically significant. Concentration of potassium was also the greatest in this group of the investigated. However, this group of athletes also possessed the greatest values of toxic elements (plumbum, arsenic) in their hair, comparing to the athletes of endurance sport and players. In their hair, hydrargyrum concentration was significantly greater comparing to the values of this element in players' hair. The greatest mean values of cadmium were established in players' hair.