Responses of tree growth and biomass production to nutrient addition in a semi‐deciduous tropical forest in Africa

Abstract

Experimental evidence of nutrient limitations on primary productivity in Afrotropical forests is rare and globally underrepresented yet are crucial for understanding constraints to terrestrial carbon uptake. In an ecosystem‐scale nutrient manipulation experiment, we assessed the early responses of tree growth rates among different tree sizes, taxonomic species, and at a community level in a humid tropical forest in Uganda. Following a full factorial design, we established 32 (eight treatments × four replicates) experimental plots of 40 × 40 m each. We added nitrogen (N), phosphorus (P), potassium (K), their combinations (NP, NK, PK, and NPK), and control at the rates of 125 kg N ha−1 year−1, 50 kg P ha−1 year−1 and 50 kg K ha−1 year−1, split into four equal applications, and measured stem growth of more than 15,000 trees with diameter at breast height (dbh) ≥1 cm. After 2 years, the response of tree stem growth to nutrient additions was dependent on tree sizes, species and leaf habit but not community wide. First, tree stem growth increased under N additions, primarily among medium‐sized trees (10–30 cm dbh), and in trees of Lasiodiscus mildbraedii in the second year of the experiment. Second, K limitation was evident in semi‐deciduous trees, which increased stem growth by 46% in +K than –K treatments, following a strong, prolonged dry season during the first year of the experiment. This highlights the key role of K in stomatal regulation and maintenance of water balance in trees, particularly under water‐stressed conditions. Third, the role of P in promoting tree growth and carbon accumulation rates in this forest on highly weathered soils was rather not pronounced; nonetheless, mortality among saplings (1–5 cm dbh) was reduced by 30% in +P than in –P treatments. Although stem growth responses to nutrient interaction effects were positive or negative (likely depending on nutrient combinations and climate variability), our results underscore the fact that, in a highly diverse forest ecosystem, multiple nutrients and not one single nutrient regulate tree growth and aboveground carbon uptake due to varying nutrient requirements and acquisition strategies of different tree sizes, species, and leaf habits.

Phosphorus scarcity contributes to nitrogen limitation in lowland tropical rainforests

“…A meta‐analysis of 48 tropical lowland and montane forests located in the Neotropics, Hawaii, and Southeast Asia highlights the existence of generalized N and P limitations (Wright, 2019). Conversely, a fertilizing experiment conducted in Africa showed N to be more relevant than P on stem growth (Manu et al, 2022). The same pattern of co‐limitation has also often been commonly documented in terrestrial, freshwater, and marine environments (Elser et al, 2007).…”

Nutrient fluxes via litterfall in silver birch (Betula pendula Roth) stands growing on post-arable soils

“…Although N generally occurs in litterfall in relatively high amounts, it is usually a de cient element in forest ecosystems--one of the major factors limiting biomass production in terrestrial biomes (Cánovas et al 2018;Manu et al 2022). The N content varies strongly among litterfall fractions, re ecting the effects of several factors.…”

Nutrient fluxes via litterfall in silver birch (Betula pendula Roth) stands growing on post-arable soils

“…Although N generally occurs in litterfall in relatively high amounts, it is usually a deficient element in forest ecosystems--one of the major factors limiting biomass production in terrestrial biomes (Cánovas et al 2018;Manu et al 2022). The N content varies strongly among litterfall fractions, reflecting the effects of several factors.…”