Responses of two barley cultivars differing in their salt tolerance to moderate and high salinities and subsequent recovery

Abstract

Two barley (Hordeum vulgare L.) cultivars, Czech spring cv. Amulet and Syrian landrace Tadmor, were subject to different salinity treatments: 1) the NaCl concentration was gradually increased from 0 (the control) to either 100 (a moderate salt stress) or 300 mM NaCl (a high salt stress), 2) the NaCl concentration was increased directly either from 0 to 300 mM NaCl or from 100 to 300 mM NaCl, and 3) a recovery when all variants were transferred back to control conditions and cultivated for seven additional days before sampling. The following parameters were determined: water saturation deficit (WSD), osmotic potential (ψ s ), leaf proline content, maximum quantum yield of photosystem (PS) II photochemistry (measured as variable to maximum chlorophyll a fluorescence ratio, F v /F m ), and relative accumulation of dehydrins (DHN). Both quantitative and qualitative differences in dehydrins were found between NaCl-treated Amulet and Tadmor. A principal component analysis (PCA) of all experiment data revealed a differential ability of Amulet and Tadmor to recover after the 300 mM NaCl treatments indicating better salt tolerance in Tadmor. Correlation analyses have shown statistically significant correlations between WSD, ψ s , proline, and DHN.

Proteomic Response of Hordeum vulgare cv. Tadmor and Hordeum marinum to Salinity Stress: Similarities and Differences between a Glycophyte and a Halophyte

“…The hydroponical solution was changed every 3rd day in order to prevent nutrient depletion. All plants were first exposed to the control treatment of 0.2 mM NaCl (osmotic potential of the control solution ψ = -0.095 MPa) for 7 days similarly to previous publications ( Islam et al, 2007 ; Kosová et al, 2015 ) due to a halophytic nature of H. marinum . NaCl was added to the hydroponical solution every day in a gradually increasing manner by 50 mM NaCl up to the final concentrations of 100 mM NaCl (100; a moderate salt stress ψ = -0.608 MPa) and 300 mM NaCl (300; a high salt stress ψ = -1.64 MPa), respectively.…”

“…Determination of WSD, osmotic potential (ψ s ), proline accumulation, chlorophyll fluorescence parameter maximum quantum yield of photosystem II photochemistry ( F v / F m ) and dehydrin relative accumulation was performed as described previously in Kosová et al (2015) . Youngest fully developed leaves were used for the analyses.…”

Effects of salt stress on eco-physiological characteristics in Robinia pseudoacacia based on salt-soil rhizosphere

“…Therefore, the decrease of water uptake of roots due to decreasing RA led to the deficits of R. pseudoacacia seedlings. An increase in the resistance to water flow from soil to plant is common in saline conditions (Farissi et al, 2013;Kosová et al, 2014;Li et al, 2016;Ranjit et al, 2016;Álvarez and Sánchez-Blanco, 2014). In this paper, roots suffered the most severe water deficit.…”

The Influence of Endogenous Cytokinins on Methiljasmonat-Induced Protection on Wheat Plants

“…Предобработка фитогормонами, наиболее заметно в случае БАП в начальный период стресса, способствовала интенсификации повышения содержания пролина в растениях в ответ на ПЭГ. Литературные данные свидетельствуют об индукции осмотическим стрессом накопления пролина и глицинбетаина в злаках и их участии в стабилизации антиоксидантных ферментов [Kosová et al, 2015]. Накопление различных осморегулирующих веществ, в частности пролина в цитоплазме, восстанавливает осмотический потенциал растений, благодаря чему нормализуется снабжение клеток водой и приходит в норму растяжимость клеток.…”