Responses of Unsaturated Fatty Acid in Membrane Lipid and Antioxidant Enzymes to Chilling Stress in Sweet Sorghum (Sorghum bicolor (L.) Moench) Seedling

Guo, Yuanyuan; Liu, Shanshan; Yang, Zhen; Tian, Shengke; Sui, Na

doi:10.5539/jas.v8n9p71

Cited by 9 publications

(5 citation statements)

References 55 publications

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“…Unsaturated fatty acids constitute the major components of membrane lipids of both prokaryotes and eukaryotic organisms [5]. Previous studies showed that fatty acid compositions were altered during plants exposed to cold temperatures [36,37], and the level of unsaturated fatty acids is associated with cold tolerance [6,7]. In our study, transcript variants were involved in C18 unsaturated fatty acid biosynthesis pathway (Additional file 9: Table S6), and C18 unsaturated fatty acid contents were enhanced by cold stress (Fig.…”

Section: Discussionsupporting

confidence: 50%

SMRT sequencing of a full-length transcriptome reveals transcript variants involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in Pennisetum giganteum

Xiang

et al. 2020

BMC Genomics

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Background: Pennisetum giganteum, an abundant, fast-growing perennial C 4 grass that belongs to the genus Pennisetum, family Poaceae, has been developed as a source of biomass for mushroom cultivation and production, as a source of forage for cattle and sheep, and as a tool to remedy soil erosion. However, having a chilling-sensitive nature, P. giganteum seedlings need to be protected while overwintering in most temperate climate regions. Results:To elucidate the cold stress responses of P. giganteum, we carried out comprehensive full-length transcriptomes from leaf and root tissues under room temperature (RT) and chilling temperature (CT) using PacBio Iso-Seq long reads. We identified 196,124 and 140,766 full-length consensus transcripts in the RT and CT samples, respectively. We then systematically performed functional annotation, transcription factor identification, long noncoding RNAs (lncRNAs) prediction, and simple sequence repeat (SSR) analysis of those full-length transcriptomes. Isoform analysis revealed that alternative splicing events may be induced by cold stress in P. giganteum, and transcript variants may be involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in P. giganteum. Furthermore, the fatty acid composition determination and gene expression level analysis supported that C18 unsaturated fatty acid biosynthesis and metabolism pathways may play roles during cold stress in P. giganteum. Conclusions:We provide the first comprehensive full-length transcriptomic resource for the abundant and fastgrowing perennial grass Pennisetum giganteum. Our results provide a useful transcriptomic resource for exploring the biological pathways involved in the cold stress responses of P. giganteum.

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Section: Discussionsupporting

confidence: 50%

SMRT sequencing of a full-length transcriptome reveals transcript variants involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in Pennisetum giganteum

Xiang

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Unsaturated fatty acids constitute the major components of membrane lipids of both prokaryotes and eukaryotic organisms [5]. Previous studies showed that fatty acid compositions were altered during plants exposed to cold temperatures [36,37], and the level of unsaturated fatty acids is associated with cold tolerance [6,7]. In our study, transcript variants were involved in C18 unsaturated fatty acid biosynthesis pathway ( Supplementary Table 6), and C18 unsaturated fatty acid contents were enhanced by cold stress (Figure 7).…”

Section: Giganteumsupporting

confidence: 58%

SMRT Sequencing of a Full-length Transcriptome Reveals Transcript Variants Involved in C18 Unsaturated Fatty Acid Biosynthesis and Metabolism Pathways at Chilling Temperature in Pennisetum giganteum

Xiang²,

et al. 2019

Preprint

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Background Pennisetum giganteum, an abundant, fast-growing perennial C4 grass that belongs to the genus Pennisetum, family Poaceae, has been developed as a source of biomass for mushroom cultivation and production, as a source of forage for cattle and sheep, and as a tool to remedy soil erosion. However, having a chilling-sensitive nature, P. giganteum seedlings need to be protected while overwintering in most temperate climate regions. Results To elucidate the cold stress responses of P. giganteum, we carried out comprehensive full-length transcriptomes from leaf and root tissues under room temperature (RT) and chilling temperature (CT) using PacBio Iso-Seq long reads. We identified 196,124 and 140,766 full-length consensus transcripts in the RT and CT samples, respectively. We then systematically performed functional annotation, transcription factor identification, long non-coding RNAs (lncRNAs) prediction, and simple sequence repeat (SSR) analysis of those full-length transcriptomes. Isoform analysis revealed that alternative splicing events may be induced by cold stress in P. giganteum, and transcript variants may be involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in P. giganteum. Furthermore, the fatty acid composition determination and gene expression level analysis supported that C18 unsaturated fatty acid biosynthesis and metabolism pathways may play roles during cold stress in P. giganteum. Conclusions We provide the first comprehensive full-length transcriptomic resource for the abundant and fast-growing perennial grass Pennisetum giganteum. Our results provide a useful transcriptomic resource for exploring the biological pathways involved in the cold stress responses of P. giganteum.

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“…In several plant species, low temperature (LT) also increases ROS and induces oxidative stress. For example, Guo et al [ 105 ] exposed two sweet sorghum inbred lines ( Sorghum bicolor L. cv. M81-E and Roma) to LT (10 °C; 0, 12, 24, 36, and 48 h) and observed that MDA content increased to a maximum in both M81-E and Roma by 266% after 48 h of LT stress.…”

Section: Oxidative Stress Under Abiotic Stressmentioning

confidence: 99%

Regulation of ROS Metabolism in Plants under Environmental Stress: A Review of Recent Experimental Evidence

Hasanuzzaman

Bhuyan

Parvin

et al. 2020

IJMS

303

146

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Various environmental stresses singly or in combination generate excess amounts of reactive oxygen species (ROS), leading to oxidative stress and impaired redox homeostasis. Generation of ROS is the obvious outcome of abiotic stresses and is gaining importance not only for their ubiquitous generation and subsequent damaging effects in plants but also for their diversified roles in signaling cascade, affecting other biomolecules, hormones concerning growth, development, or regulation of stress tolerance. Therefore, a good balance between ROS generation and the antioxidant defense system protects photosynthetic machinery, maintains membrane integrity, and prevents damage to nucleic acids and proteins. Notably, the antioxidant defense system not only scavenges ROS but also regulates the ROS titer for signaling. A glut of studies have been executed over the last few decades to discover the pattern of ROS generation and ROS scavenging. Reports suggested a sharp threshold level of ROS for being beneficial or toxic, depending on the plant species, their growth stages, types of abiotic stresses, stress intensity, and duration. Approaches towards enhancing the antioxidant defense in plants is one of the vital areas of research for plant biologists. Therefore, in this review, we accumulated and discussed the physicochemical basis of ROS production, cellular compartment-specific ROS generation pathways, and their possible distressing effects. Moreover, the function of the antioxidant defense system for detoxification and homeostasis of ROS for maximizing defense is also discussed in light of the latest research endeavors and experimental evidence.

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Responses of Unsaturated Fatty Acid in Membrane Lipid and Antioxidant Enzymes to Chilling Stress in Sweet Sorghum (Sorghum bicolor (L.) Moench) Seedling

Cited by 9 publications

References 55 publications

SMRT sequencing of a full-length transcriptome reveals transcript variants involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in Pennisetum giganteum

SMRT sequencing of a full-length transcriptome reveals transcript variants involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in Pennisetum giganteum

SMRT Sequencing of a Full-length Transcriptome Reveals Transcript Variants Involved in C18 Unsaturated Fatty Acid Biosynthesis and Metabolism Pathways at Chilling Temperature in Pennisetum giganteum

Regulation of ROS Metabolism in Plants under Environmental Stress: A Review of Recent Experimental Evidence

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