2011
DOI: 10.1016/j.plaphy.2011.02.007
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Responses to desiccation injury in developing wheat embryos from naturally- and artificially-dried grains

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Cited by 13 publications
(9 citation statements)
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“…Ascorbate peroxidase (APX) activity was measured according to Nakano and Asada (1981) with modification as in Spanò et al (2011a). Enzyme activity was assayed from the decrease in absorbance at 290 nm (extinction coefficient 2.8 mM −1 cm −1 ) as ascorbate was oxidized and the unit of activity was expressed as micromole of ascorbic acid oxidized min –1 .…”
Section: Methodsmentioning
confidence: 99%
“…Ascorbate peroxidase (APX) activity was measured according to Nakano and Asada (1981) with modification as in Spanò et al (2011a). Enzyme activity was assayed from the decrease in absorbance at 290 nm (extinction coefficient 2.8 mM −1 cm −1 ) as ascorbate was oxidized and the unit of activity was expressed as micromole of ascorbic acid oxidized min –1 .…”
Section: Methodsmentioning
confidence: 99%
“…The lower germination presented by Núcleo Bandeirante accession after the desiccation is probably due to the fact of its greater sensitivity to desiccation. According Spanó et al (2011) [28] different protective mechanisms seem to act in combination but their relative importance changes as maturation progresses and in response to the drying method. There was formation of normal seedlings, with well-developed root and shoots and with no apparent damage at 60 days of in vitro culture in both accessions.…”
Section: Discussionmentioning
confidence: 99%
“…Tolerance to dehydration varies from species to species and among tissues of the same plant, and appropriate humidity must be reached to ensure adequate survival of the material [22]. Besides that, desiccation tolerance is directly related to changes in cellular components, to the activation of antioxidant enzyme systems, to oligosaccharides and to heat-resistant proteins [3,8,28].…”
Section: Introductionmentioning
confidence: 99%
“…Mecanismos como o acúmulo de sacarose e oligossacarídeos da família da rafinose (PUKACKA et al, 2009) e de proteínas proteínas LEA (BATTAGLIA et al, 2008;SPANÒ et al, 2011) estão interligados e operam sob um rígido controle genético (ANGELOVICI et al, 2010), cuja completa elucidação ainda não foi alcançada.…”
Section: Espécieunclassified