Responses to K deficiency and waterlogging interact via respiratory and nitrogen metabolism

Cui, Jing; Abadie, Cyril; Carroll, Adam J.; Lamade, Emmanuelle; Tcherkez, Guillaume

doi:10.1111/pce.13450

Cited by 33 publications

(50 citation statements)

References 63 publications

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“…Changes in organic acids were accompanied by a modification of amino acid metabolism, with a decrease in asparagine and serine, and in the content of alanine aminotransferase. Accordingly, we previously showed that waterlogging inhibited N assimilation in leaves (Cui et al ., ). It is worth noting that at high K, waterlogging was also associated with generally less amino acids in rachis, suggesting an inhibition of N assimilates export from leaves (Fig.…”

Section: Discussionmentioning

confidence: 97%

Metabolic responses to potassium availability and waterlogging reshape respiration and carbon use efficiency in oil palm

et al. 2019

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Summary Oil palm is by far the major oil‐producing crop on the global scale, with c. 62 Mt oil produced each year. This species is a strong potassium (K)‐demanding species cultivated in regions where soil K availability is generally low and waterlogging due to tropical heavy rains can limit further nutrient absorption. However, the metabolic effects of K and waterlogging have never been assessed precisely. Here, we examined the metabolic response of oil palm saplings in the glasshouse under controlled conditions (nutrient composition with low or high K availability, with or without waterlogging), using gas exchange, metabolomics and proteomics analyses. Our results showed that both low K and waterlogging have a detrimental effect on photosynthesis but stimulate leaf respiration, with differential accumulation of typical metabolic intermediates and enzymes of Krebs cycle and alternative catabolic pathways. In addition, we found a strong relationship between metabolic composition, the rate of leaf dark respiration, and cumulated respiratory loss. Advert environmental conditions (here, low K and waterlogging) therefore have an enormous effect on respiration in oil palm. Leaf metabolome and proteome appear to be good predictors of carbon balance, and open avenues for cultivation biomonitoring using functional genomics technologies.

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Section: Discussionmentioning

confidence: 97%

Metabolic responses to potassium availability and waterlogging reshape respiration and carbon use efficiency in oil palm

et al. 2019

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“…There are many experimental works in the literature that show how a single abiotic stress can affect N uptake and assimilation at the physiological, biochemical, but also at gene expression level (Hemon et al, 1990;Mungur et al, 2005;Ashraf et al, 2018). However, very little information can be found about how the combination of two or more abiotic stresses may affect this important metabolic process (Cui et al, 2019). In recent years, an increase in the experiments in the field of abiotic stress combination has been observed, most probably due to the current undeniable effects of climate change on our agriculture and to the fact that in nature, abiotic stresses always act in combination.…”

Section: Discussionmentioning

confidence: 99%

“…At present, there are very little information about the effect of abiotic stress combination on the N uptake and assimilation pathway (Cui et al, 2019). Thus, in order to identify agronomic strategies that can be used to fight against the consequences of climate change in crops, detailed studies related to nitrogen metabolism should be developed at the physiological, biochemical, and molecular levels, which is also the primary metabolism related to growth and production in plants.…”

Section: Introductionmentioning

confidence: 99%

Using Tomato Recombinant Lines to Improve Plant Tolerance to Stress Combination Through a More Efficient Nitrogen Metabolism

et al. 2020

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The development of plant varieties with a better nitrogen use efficiency (NUE) is a means for modern agriculture to decrease environmental pollution due to an excess of nitrate and to maintain a sufficient net income. However, the optimum environmental conditions for agriculture will tend to be more adverse in the coming years, with increases in temperatures, water scarcity, and salinity being the most important productivity constrains for plants. NUE is inherently a complex trait, as each step, including N uptake, translocation, assimilation, and remobilization, is governed by multiple interacting genetic and environmental factors. In this study, two recombinant inbred lines (RIL-66 and RIL-76) from a cross between Solanum lycopersicum and Solanum pimpinellifoilum with different degree of tolerance to the combination of salinity and heat were subjected to a physiological, ionomic, amino acid profile, and gene expression study to better understand how nitrogen metabolism is affected in tolerant plants as compared to sensitive ones. The ionomics results showed a different profile between the two RILs, with K + and Mg 2+ being significantly lower in RIL-66 (low tolerant) as compared to RIL-76 (high tolerant) under salinity and heat combination. No differences were shown between the two RILs in N total content; however, N-NO 3 − was significantly higher in RIL-66, whereas N-N org was lower as compared to the other genotype, which could be correlated with its tolerance to the combination of salinity and heat. Total proteins and total amino acid concentration were significantly higher in RIL-76 as compared to the sensitive recombinant line under these conditions. Glutamate, but more importantly glutamine, was also highly synthesized and accumulated in RIL-76 under the combination of salinity and heat, which was in agreement with the upregulation of the nitrogen metabolism related transcripts studied (SlNR, SlNiR, SlGDH, SlGLT1, SlNRT1.2, SlAMT1, and SlAMT2). This study emphasized the importance of studying abiotic stress in combination and how recombinant material with different degrees of tolerance can be

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“…That is, quite counter‐intuitively, K + deficiency implies an extra demand in negative charges to reach electro‐neutrality, which is met by accumulated organic and amino acids (Armengaud et al, ). The excess of positive charges mostly comes from the considerable increase in Ca 2+ (up to twofold increase) and Mg 2+ (more than twofold) in oil palm and sunflower (Cui, Abadie, et al, ; Cui, Davanture, et al, ). Under K + deficiency, there is also an increase in the difference between Ca 2+ and the sum Mg 2+ + K + (of about 0.4 mmol positive charges g −1 DW in Figure ).…”

Section: Putrescine and Regulation Of Cation Transport And Balancementioning

confidence: 99%

“…This still needs to be tested under K + deficiency, based on large amounts of putrescine accumulated naturally. However, metabolomics analyses have suggested that the increased CO 2 release under K + deficiency is not associated with a higher ATP production but rather reflects lower efficiency of the TCAP when K + is limiting enzymatic activity (Cui, Abadie, et al, ). Also, it should be noted that mitochondrial carbonic anhydrase, which might play an important role in anaplerosis (conversion of catabolic CO 2 into bicarbonate), is inhibited with a high affinity (low K i ) by spermine and spermidine, while putrescine has no effect (Carta et al, ).…”

Section: Roles Of Putrescine In Mitochondriamentioning

confidence: 99%

What is the role of putrescine accumulated under potassium deficiency?

Cui

Pottosin

Lamade

et al. 2020

Plant Cell & Environment

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Biomarker metabolites are of increasing interest in crops since they open avenues for precision agriculture, whereby nutritional needs and stresses can be monitored optimally. Putrescine has the potential to be a useful biomarker to reveal potassium (K + ) deficiency. In fact, although this diamine has also been observed to increase during other stresses such as drought, cold or heavy metals, respective changes are comparably low. Due to its multifaceted biochemical properties, several roles for putrescine under K + deficiency have been suggested, such as cation balance, antioxidant, reactive oxygen species mediated signalling, osmolyte or pH regulator. However, the specific association of putrescine build-up with low K + availability in plants remains poorly understood, and possible regulatory roles must be consistent with putrescine concentration found in plant tissues. We hypothesize that the massive increase of putrescine upon K + starvation plays an adaptive role. A distinction of putrescine function from that of other polyamines (spermine, spermidine) may be based either on its specificity or (which is probably more relevant under K + deficiency) on a very high attainable concentration of putrescine, which far exceeds those for spermidine and spermine. putrescine and its catabolites appear to possess a strong potential in controlling cellular K + and Ca 2+ , and mitochondria and chloroplasts bioenergetics under K + stress. K E Y W O R D Sdeficiency, ion balance, polyamines, potassium, putrescine

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Responses to K deficiency and waterlogging interact via respiratory and nitrogen metabolism

Cited by 33 publications

References 63 publications

Metabolic responses to potassium availability and waterlogging reshape respiration and carbon use efficiency in oil palm

Metabolic responses to potassium availability and waterlogging reshape respiration and carbon use efficiency in oil palm

Using Tomato Recombinant Lines to Improve Plant Tolerance to Stress Combination Through a More Efficient Nitrogen Metabolism

What is the role of putrescine accumulated under potassium deficiency?

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