Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusion and an E. coli strain carrying a grpE'::lux fusion are similar

Dyk, Tina K. Van; Smulski, Dana R.; Reed, T. R.; Belkin, Shimshon; Vollmer, Amy Cheng; LaRossa, Robert A.

doi:10.1128/aem.61.11.4124-4127.1995

Cited by 82 publications

(31 citation statements)

References 14 publications

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“…Another possibility would be that methanol increases protein denaturation. Like ethanol (28), whose known effects include protein unfolding (4, 21), methanol can induce the transcription of heat shock genes in E. coli (29). This is an attractive idea because denaturation is also one of the effects of oxidative stress on proteins (6), providing a link between the two phenotypes described above.…”

Section: Figmentioning

confidence: 99%

The l -Isoaspartyl Protein Repair Methyltransferase Enhances Survival of Aging Escherichia coli Subjected to Secondary Environmental Stresses

1998

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Like its homologs throughout the biological world, thel-isoaspartyl protein repair methyltransferase ofEscherichia coli, encoded by the pcm gene, can convert abnormal l-isoaspartyl residues in proteins (which form spontaneously from asparaginyl or aspartyl residues) to normal aspartyl residues. Mutations in pcm were reported to greatly reduce survival in stationary phase and when cells were subjected to heat or osmotic stresses (C. Li and S. Clarke, Proc. Natl. Acad. Sci. USA 89:9885–9889, 1992). However, we subsequently demonstrated that those strains had a secondary mutation inrpoS, which encodes a stationary-phase-specific sigma factor (J. E. Visick and S. Clarke, J. Bacteriol. 179:4158–4163, 1997). We now show that the rpoS mutation, resulting in a 90% decrease in HPII catalase activity, can account for the previously observed phenotypes. We further demonstrate that a new pcmmutant lacks these phenotypes. Interestingly, the newly constructedpcm mutant, when maintained in stationary phase for extended periods, is susceptible to environmental stresses, including exposure to methanol, oxygen radical generation by paraquat, high salt concentrations, and repeated heating to 42°C. The pcmmutation also results in a competitive disadvantage in stationary-phase cells. All of these phenotypes can be complemented by a functionalpcm gene integrated elsewhere in the chromosome. These data suggest that protein denaturation and isoaspartyl formation may act synergistically to the detriment of aging E. coli and that the repair methyltransferase can play a role in limiting the accumulation of the potentially disruptive isoaspartyl residues in vivo.

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Section: Figmentioning

confidence: 99%

The l -Isoaspartyl Protein Repair Methyltransferase Enhances Survival of Aging Escherichia coli Subjected to Secondary Environmental Stresses

1998

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“…Currently, we are applying this bioreactor to wastewater components such as heavy metals and toxic chemicals (nitrophenols, pentachlorophenol, etc.). Such compounds have been demonstrated to induce a response in this bioluminescent E. coli strain (Van Dyk et al, 1995). This miniature bioreactor continues to work in a stable and reliable manner.…”

Section: Discussionmentioning

confidence: 99%

A Miniature Bioreactor for Sensing Toxicity Using Recombinant Bioluminescent Escherichia coli Cells

Gu¹,

Dhurjati²,

Dyk³

et al. 1996

Biotechnol. Prog.

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A miniature bioreactor was fabricated as a contactor between biosensing cells and toxic materials. This miniature bioreactor (58 mL working volume) showed performance similar to that of a conventional bioreactor, as well as the advantages of easy installation, facile operation, and small medium requirements during long-term continuous operation. A performance evaluation measured the response to ethanol in continuous operation by using a recombinant bioluminescent Escherichia coli strain. Continuous cultures were repeatedly induced by the ethanol challenge. Steady-state cell concentrations (OD) were found to be decreased, the induced specific bioluminescence (SBL) peak value was found to be increased, and the peak response time, which is the time constant of this continuous monitoring system, was found to be decreased with increasing dilution rate. Finally on- and off-line bioluminescence monitoring was shown to be reliable, suggesting that this system is suitable for applications such as monitoring the influent and effluent streams of waste water biotreatment plants.

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“…E. coli JF699 [lacY29 proC24 tsx-63 purE41 Ϫ ompA252 his-53 rpsL97 (strR) xyl-14 metB65 cycA1 cycB2 ilv-277] (11) and E. coli SB1803 [thr-1 ara-14 leuB6 ⌬(gpt-proA)62 lacY1 supE44 galK12 Ϫ rac hisC3 rfbD1 metG83 rpsL25 kdgK51 xyl-5 mtl-1 thi-1 lpcB] (5) were provided by B. J. Bachmann (E. coli Genetic Stock Center, Yale University, New Haven, Conn.). E. coli DPD1006 containing the lonЈ::lux (rpoH-controlled protease) fusion plasmid pLonLux2 (25,27), E. coli DPD2511 containing the katGЈ::lux (oxyR-controlled catalase) fusion plasmid pKatGLux2 (3,25), E. coli DPD2519 containing the micFЈ::lux (in the soxRS regulon; responsive to superoxides) fusion plasmid pMicFLux1 (3,25), E. coli DPD2540 containing the fabAЈ::lux (a ␤-hydroxydecanoylthioester dehydrase gene under fadR control) fusion plasmid pFabALux6 (4,25), E. coli DE135 containing the uspAЈ::lux (universal stress) fusion plasmid pUspALux2 (25,28), and E. coli TV1068 containing the lacЈ::lux (␤-galactosidase) fusion plasmid pLacLux (25) were kindly provided by S. Belkin (Hebrew University, Jerusalem, Israel) and R. A. LaRossa. These plasmids are based on the pUCD615 plasmid (18) carrying the V. fischeri promoterless luxCDABE genes fused to the above-mentioned promoter elements.…”

Section: Methodsmentioning

confidence: 99%

Identification and Quantification of Toxic Chemicals by Use of Escherichia coli Carrying lux Genes Fused to Stress Promoters

Ben-Israel

Ulitzur

1998

Appl Environ Microbiol

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The luxCDABE bioluminescence genes of the Vibrio fischeri lux system have been used as a reporter system for different stress and regulatory promoters of Escherichia coli. Selected E. coli strains carryinglux genes fused to different promoters were exposed to various toxic chemicals, and the recorded luminescence was used for the characterization of the biologic signature of each compound. Analysis of these data with the aid of a proper algorithm allowed quantitative and qualitative assessment of toxic chemicals. Of the 25 tested chemicals, 23 were identified by this novel strategy in a 3-h procedure. This system can also be adapted for the identification of simple mixtures of toxic agents when the biologic signatures of the individual compounds are known. This biologic recognition strategy also provides a tool for evaluating the degree of similarity between the modes of action of different toxic agents.

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Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusion and an E. coli strain carrying a grpE'::lux fusion are similar

Cited by 82 publications

References 14 publications

The l -Isoaspartyl Protein Repair Methyltransferase Enhances Survival of Aging Escherichia coli Subjected to Secondary Environmental Stresses

The l -Isoaspartyl Protein Repair Methyltransferase Enhances Survival of Aging Escherichia coli Subjected to Secondary Environmental Stresses

A Miniature Bioreactor for Sensing Toxicity Using Recombinant Bioluminescent Escherichia coli Cells

Identification and Quantification of Toxic Chemicals by Use of Escherichia coli Carrying lux Genes Fused to Stress Promoters

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