Responsiveness of Experimental Surgical-Wound Infections to Topical Chemotherapy

Abstract

Topical agents freshly formulated in a cream base vehicle as well as commercial topical preparations were used to evaluate in mice the responsiveness of experimental surgical wounds infected with Staphylococcus aureus or Pseudomonas aeruginosa to chemotherapy. The responsiveness of the infections to therapy or the efficacy of a topical agent was assessed primarily by means of wound counts of the infecting organism before and after the employment of an immediate (prophylactic) or delayed (therapeutic) treatment regimen. From tests of several concentrations of an agent formulated in the vehicle, a median effective dose could be determined. In the case of the lethal P. aeruginosa infection, a median protective dose could be determined. Both infections were found to be quite susceptible to treatment with those topical agents that demonstrated good activity in vitro against the test organisms. The results of the investigation indicated that the model infections were suitable for the screening of potential topical agents in vivo.In the preceding paper experimental surgical-wound infections in mice were described and characterized (6). In addition, a rapid method for quantitating bacteria in the wound infections was presented. In this paper the response of the surgical-wound infections to chemotherapy with various topical antimicrobial agents is explored. Freshly formulated antibacterial agents, commonly administered topically, as well as commercial topical antimicrobial products were employed in the study. These topical formulations were used not only for evaluating the responsiveness of the experimental infections to chemotherapy but also for demonstrating how, using standardized methodology, the model infections can be employed to assess various measures oftopical efficacy.MATERIALS AND METHODS Antibacterial agents. The antibiotics and chemicals used in this study were gentamicin sulfate, 634 ,ug/mg (Schering Corp.); polymyxin B sulfate, 7,956

Characterization and Quantitation of Experimental Surgical-Wound Infections Used to Evaluate Topical Antibacterial Agents

“…Many experimental models fail to meet the requirements that are necessary for a model to be truly relevant. The results described in this study and those presented in the following paper (13) indicate that most of the criteria are met in the surgical-wound model. It should be noted, however, that the extensive systemic involvement resulting from the experimental wound infections is rarely observed in clinical cases.…”

“…The application of the model infection to the evaluation of topical antibacterial agents is described in the following paper (13).…”

A comparative study of the effect of different topical agents on burn wound infections

“…Although there is some controversy as to the effectiveness of topical chemotherapy in certain types of local infections, there are several clinical situations in which topical therapy is apparently beneficial. [17] Topical antimicrobial formulations are widely used in the field of dermatology. [17] The primary purpose of the present study was to compare the various topical antimicrobial agents and the preparations, most of which are employed in the hospitals.…”

In-vitro Activity of Honey and Topical Silver in Wound Care Management

“…A lot of controversy exist between the effectiveness of the topical agents but still the topical agents has been proved to have more beneficial effects [21]. Topical applications are most commonly used for many dermatological conditions [21].…”

“…A lot of controversy exist between the effectiveness of the topical agents but still the topical agents has been proved to have more beneficial effects [21]. Topical applications are most commonly used for many dermatological conditions [21]. The purpose of the study was to evaluate the in-vitro efficacy of 2 most widely used topical agents honey and silver nitrate and to know their effect when incorporated in wound dressings in the form of coatings for faster healing of wounds.…”