Responsiveness of rabbits to superovulation treatment by a single injection of follicle‐stimulating hormone with aluminum hydroxide gel

Hashimoto, Shu; Kimura, Koji; Kuramochi, Takashi; Aoyagi, Kazuki; Hirako, Makoto; Kawaguchi, Mamoru; Iwata, Hisataka; Hirao, Masao; Kitada, Kensaku; Hirasawa, Kazuo; Ueda, Masatsugu

doi:10.1002/mrd.20672

Cited by 12 publications

(7 citation statements)

References 16 publications

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“…On the basis of the rapid clearance of FSH (w5 hours) [39], regardless of the dose administered [55], an alternative could be to modify hormone absorption. Modifications examined have included changing the route administration (subcutaneous injection, [55][56][57]) or mixing the pFSH with polymers, such as polyvinylpyrrolidone [58], aluminum hydroxide gel [59] or HA [42] to reduce the rate of absorption. Tríbulo et al [42] reported satisfactory superovulatory results after treating Table 1 Summary of cumulus-oocyte complex yield and in vitro embryo production (mean AE standard error or percentage) in nonlactating Holstein cows that were not treated (control), treated with 200 mg of porcine FSH (pFSH; F200) in twice-daily injections or 200 or 300 mg of pFSH as a single injection in 0.5% hyaluronan (F200HA and F300HA).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Vieira¹,

Rodrigues

Netto

et al. 2016

Theriogenology

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Plasma FSH profiles, in vitro embryo production (IVP) after ovum pickup (OPU), and establishment of pregnancy with IVP embryos were compared in untreated Holstein oocyte donors and those superstimulated with multiple injections or a single intramuscular (IM) injection of porcine FSH (pFSH) in hyaluronan (HA). Plasma FSH profiles were determined in 23 heifers randomly allocated to one of four groups. Controls received no treatment, whereas the F200 group received 200 mg of pFSH in four doses, 12 hours apart. The F200HA and F300HA groups received 200- or 300-mg pFSH in 5 mL or 7.5 mL, respectively of a 0.5% HA solution by a single IM injection. Plasma FSH levels were determined before the first pFSH treatment and every 6 hours over 96 hours. All data were analyzed by orthogonal contrasts. Circulating FSH area under curve (AUC) in pFSH-treated animals was greater than that in the control group (P = 0.02). Although the AUC did not differ among FSH-treated groups (P = 0.56), the total period with elevated plasma FSH was greater in the F200 group than in the HA groups (P < 0.0001). However, the F300HA group had a greater AUC than the F200HA group (P = 0.006), with a similar total period with elevated plasma FSH (P = 0.17). The IVP was performed in 90 nonlactating Holstein cows randomly allocated to one of the four treatment groups as in the first experiment. A greater proportion of medium-sized (6-10 mm) follicles was observed in cows receiving pFSH, regardless of the treatment group (P < 0.0001). Also, numbers of follicles (P = 0.01), cumulus-oocyte complexes (COCs) retrieved (P = 0.01) and matured (P = 0.02), cleavage rates (P = 0.002), and blastocysts produced per OPU session (P = 0.06) were greater in cows receiving pFSH, regardless of the treatment group. Cows in the F200HA group had a greater recovery rate (P = 0.009), number of COCs cultured (P = 0.04), and blastocysts produced per OPU session (P = 0.06) than cows in the F300HA group. Similar pregnancy rates were observed 50 to 60 days after transferring IVP embryos from donors in the different treatment groups (P > 0.05). In conclusion, a single IM injection of pFSH combined in 0.5% HA resulted in similar plasma FSH profiles as twice-daily pFSH treatments. Treatment of nonlactating donors with pFSH, with or without HA, resulted in increased IVP over untreated controls. A single dose of 200 mg of pFSH in 0.5% HA resulted in greater IVP than 300-mg pFSH in HA. Finally, pregnancy rates with IVP embryos were similar, regardless donor treatment.

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Section: Discussionmentioning

confidence: 99%

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Vieira¹,

Rodrigues

Netto

et al. 2016

Theriogenology

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“…116,117 Sustained-release formulations of FSH are also in development that could result in a decreased number of gonadotropin doses. 118 In contrast to the LH receptor, both low molecular weight agonists and antagonists have been identified for the FSH receptor. FSH receptor antagonists may be used as non-hormonal contraceptives.…”

Section: F S H R E C E P T O R L I G a N D Smentioning

confidence: 99%

G protein‐coupled receptors of the hypothalamic–pituitary–gonadal axis: A case for gnrh, LH, FSH, and GPR54 receptor ligands

Heitman

IJzerman

2008

Medicinal Research Reviews

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The hypothalamic-pituitary-gonadal (HPG) axis, important in reproduction and sex hormone-dependent diseases, is regulated by a number of G protein-coupled receptors. The recently "deorphanized" GPR54 receptor activated by the peptide metastin is thought to be the key regulator of the axis, mainly by releasing gonadotropin-releasing hormone (GnRH) from the hypothalamus. The latter decapeptide, through the activation of the GnRH receptor in the anterior pituitary, causes the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which subsequently activate their respective receptors on the gonadotrope cells. In this review we will discuss the small molecule agonists and antagonists that are currently being developed to intervene with the action of these four receptors. For GnRH receptors, 14 different chemical classes of non-peptidic antagonists have been reported, while for the LH receptor three classes of agonists have been described. Both agonists and antagonists have been introduced for the FSH receptor. Recently, the first non-peptidic agonist for GPR54 was reported.

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“…A single administration of FSH in PVP has been used to induce SOV in rabbits [56, 57]. We previously used AH-gel as an adsorbent for FSH for the SOV in rabbits [58]. We found that the numbers of total and fertilized eggs recovered from rabbits treated with FSH in AH-gel were similar to multiple FSH injections and were significantly greater than the numbers obtained from a single injection of FSH with PVP.…”

Section: Application Of a Single Administration Of Fsh In Ah-gel To Omentioning

confidence: 99%

Superovulation with a single administration of FSH in aluminum hydroxide gel: a novel superovulation method for cattle

Kimura

2016

Journal of Reproduction and Development

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Superovulation (SOV) is a necessary technique to produce large numbers of embryos for embryo transfer. In the conventional methods, follicular stimulating hormone (FSH) is administered to donor cattle twice daily for 3 to 4 days. As this method is labor intensive and stresses cattle, improving this method has been desired. We previously developed a novel and simple SOV method, in which the intramuscular injection of a single dose of FSH in aluminum hydroxide gel (AH-gel) induced the growth of multiple follicles, ovulation and the production of multiple embryos. Here we show that AH-gel can efficiently adsorb FSH and release it effectively in the presence of BSA, a major interstitial protein. When a single intramuscular administration of the FSH and AH-gel mixture was performed to cattle, multiple follicular growth, ovulation and embryo production were induced. However, the treatments caused indurations at the administration sites in the muscle. To reduce the muscle damage, we investigated alternative administration routes and different amounts of aluminum in the gel. By administering the FSH in AH-gel subcutaneously rather than intramuscularly, the amount of aluminum in the gel could be reduced, thus reducing the size of the induration. Moreover, repeated administrations of FSH with AH-gel did not affect the superovulatory response. These results indicate that a single administration of FSH with AH-gel is an effective, novel and practical method for SOV treatment.

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Responsiveness of rabbits to superovulation treatment by a single injection of follicle‐stimulating hormone with aluminum hydroxide gel

Cited by 12 publications

References 16 publications

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

G protein‐coupled receptors of the hypothalamic–pituitary–gonadal axis: A case for gnrh, LH, FSH, and GPR54 receptor ligands

Superovulation with a single administration of FSH in aluminum hydroxide gel: a novel superovulation method for cattle

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