Respuesta de cultivares de Musa spp. al estrés hídrico in vitro inducido con polietilenglicol 6000

Moreno-Bermúdez, Leonardo J; Reyes, Maritza; Rodríguez, Mario; Kosky, Rafael Gómez; Roque, Berkis; Chong-Pérez, Borys

doi:10.15446/rev.colomb.biote.v19n2.60405

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…It was found that the highest content of total sugars and chlorophyll levels were in SV 25VS1 with 71.55 mg equivalent to glucose/g dry vegetable and 425.35 mg m -2 (table 3 and figure 3), which corresponds to the first SV planted in the culture medium MS + ANA + BAP + PEG 25 g•L -1 , a medium with high levels of water retention. Similar results are reported by Moreno-Bermúdez et al, (2017), who found high levels of chlorophyll in in-vitro banana seedlings submitted to water deficit using PEG (Table 3).…”

Section: Resultssupporting

confidence: 90%

Selection of somaclonal variants of maracuyá (passiflora Edulis var Flavicarpa. Deneger) tolerants to water deficit

Campiño¹,

López

Hiencapié³

2020

Rev. colomb. biotecnol.

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Climate change will have an impact on the Colombian agricultural sector, by 2050 increases in temperature and distribution of erratic rainfall are expected. Passion fruit cultivation does not tolerate water deficit, it reduces flower induction, generates fruit drop and defoliation. To tackle this problem, somaclonal variants (VS) of passion fruit were selected in-vitro, seeking tolerance to water deficit. Four phases were developed: I) callogenesis, II) direct and indirect organogenesis, II) Induction and evaluation of the water deficit with Polyethylene glycol 6000 (PEG 6000) and IV) in vitro selection of VS by morphometric measurements, chlorophyll and total sugars contents. Differences in callogenesis were found with different concentrations of 2,4-D, the concentration of 2 mg • L-1 presented better results producing calluses in less time and in greater quantity (8 days, 90% of the leaf area). In indirect and direct organogenesis the medium MS + ANA + BAP (0.3: 0.6), showed significant statistical differences with respect to other means, for the variables root length (15.14 cm), stem (16.72 cm) and leaves ( 14.51 cm) and root thickness (0.76 cm) stem (1.25) and leaf width (6.75). The influence of PEG 6000 showed significant differences, the treatment with 30 g • L-1 showed the smallest leaf width, the greatest width was found in 25 g • L-1. Statistical differences were found in chlorophyll levels and total sugar contents, the highest contents were recorded in the VS 25VS1, showing the possibility of obtaining seedlings tolerant to the water deficit of passion fruit by inducing somaclonal variation.

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Section: Resultssupporting

confidence: 90%

Selection of somaclonal variants of maracuyá (passiflora Edulis var Flavicarpa. Deneger) tolerants to water deficit

Campiño¹,

López

Hiencapié³

2020

Rev. colomb. biotecnol.

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“…In recent years, betalains have received a renewed interest due to their physicochemical characteristics (high stability at wide ranges of temperature and pH) that can be of utility in many industries, as well as bioactive compounds for the prevention of some diseases [ 25 , 26 , 27 , 28 ]. Betalains are nitrogenous phenolic compounds derived from tyrosine and are exclusive to the order Caryophyllales.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Natural Pigments Production in Response to Various Stress Signals in Cell Lines of Stenocereus queretaroensis

Ceja-López

Morales-Morales

Araujo-Sánchez

et al. 2022

Plants

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Stenocereus queretaroensis (F.A.C.Weber ex Mathes.) Buxb is a cactus that has long been used as a source food in central and northern México. Its fruits, commonly called pitayas, biosynthesize high amounts of betalains. These molecules are water-soluble nitrogenous compounds; that compared to other pigments, such as anthocyanins or carotenoids, stand out for their physicochemical stability in industrial processes. Due to genetic and environmental factors involved in the biosynthesis and accumulation of secondary metabolites in plants, we tested different stress-inducing agents (elicitor, osmotic, salt, and temperature) to induce betalains accumulation in cell culture from fruits of Stenocereus queretaroensis. This work aimed to understand stress conditions that induce the metabolic pathways required for the accumulation of betalains. The results show how betacyanin concentration increases under high sugar conditions, thus affecting the expression of L-DOPA 4, 5 dioxygenase resulting in a strong dark red coloration. This suggests this enzyme is part of a rate-limiting step in betalain production. In addition, we found that betalains accumulation occurs under particular stress conditions. Cells that have a high level of betacyanins show better resistance to stress in the cell culture, as well as an overall different behavior including cell aggregation and alterations in nuclear size. Together the results shown here may provide new strategies to manipulate and mass produce the pigments from Stenocereus queretaroensis in cell culture.

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In vitro response of gamma irradiated Vanilla planifolia jacks. Shoots in the face of water stress

Ramos-Castellá,

Iglesias-Andreu,

de Lourdes Palafox-Chávez

2024

Vegetos

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Respuesta de cultivares de Musa spp. al estrés hídrico in vitro inducido con polietilenglicol 6000

Cited by 3 publications

References 29 publications

Selection of somaclonal variants of maracuyá (passiflora Edulis var Flavicarpa. Deneger) tolerants to water deficit

Selection of somaclonal variants of maracuyá (passiflora Edulis var Flavicarpa. Deneger) tolerants to water deficit

Evaluation of Natural Pigments Production in Response to Various Stress Signals in Cell Lines of Stenocereus queretaroensis

In vitro response of gamma irradiated Vanilla planifolia jacks. Shoots in the face of water stress

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