Restenosis after balloon angioplasty. A practical proliferative model in porcine coronary arteries.

Schwartz, Robert S.; Murphy, Joseph G.; Edwards, William D.; Camrud, Allan R.; Vliestra, Ronald E.; Holmes, David R.

doi:10.1161/01.cir.82.6.2190

Cited by 367 publications

(160 citation statements)

References 32 publications

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“…20,21 The femoral approach offers the advantage of bilateral injury and carries less risk of morbidity and mortality compared with the carotid artery. The time course of intimal hyperplasia in the present study is similar to that found in rabbit, 29 pig, 30 and baboon 31 models. The I/M ratios obtained are comparable to those of other animal models.…”

Section: Discussionsupporting

confidence: 89%

Mouse Model of Femoral Artery Denudation Injury Associated With the Rapid Accumulation of Adhesion Molecules on the Luminal Surface and Recruitment of Neutrophils

Roqué

Fallon

Badimón

et al. 2000

ATVB

190

200

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Abstract-Techniques of arterial injury commonly used in animals to mimic endovascular procedures are not suitable for small mouse arteries. This has limited examination of the response to arterial injury in genetically modified mice. We therefore sought to develop a model of transluminal injury to the mouse femoral artery that would be reproducible and result in substantial levels of intimal hyperplasia. Mice of the C57BL/6 strain underwent bilateral femoral artery denudation by passage of an angioplasty guidewire. Intimal hyperplasia was observed in 10% of injured arteries at 1 week, in 88% at 2 weeks, and in 90% at 4 weeks. The mean intimal-to-medial area ratio reached 1.1Ϯ0.1 at 4 weeks. No intimal proliferation was found in control sham-operated arteries. One hour after injury, the denuded surface was covered with platelets and leukocytes, predominantly neutrophils. This was associated with the accumulation of P-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1. Expression of these adhesion molecules was not seen in the underlying medial smooth muscle cells. At 24 hours, few neutrophils remained on the denuded surface. At 1 week, macrophages and platelets were present in the vessel wall, partially covered by regenerated endothelium. Transluminal wire injury to the mouse femoral artery induces abundant intimal hyperplasia formation by 2 and 4 weeks and elicits the rapid accumulation of leukocytes and adhesion molecules on the denuded luminal surface. This model will be a valuable tool to study arterial injury in genetically modified mouse models.

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Section: Discussionsupporting

confidence: 89%

Mouse Model of Femoral Artery Denudation Injury Associated With the Rapid Accumulation of Adhesion Molecules on the Luminal Surface and Recruitment of Neutrophils

Roqué

Fallon

Badimón

et al. 2000

ATVB

190

200

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“…The coronary arteries of piglets have previously proved to respond in a similar manner to the human coronary arteries after injuries (21,22). In fact the in-stent neointimal thickness, usually caused within 28 days from an injury, has been described as identical to the human restenotic neointima.…”

Section: Discussionmentioning

confidence: 94%

Effect of a novel drug-eluted balloon coated with Genistein before stent implantation in porcine coronary arteries

et al. 2008

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This is an author version of the contribution published on: Questa è la versione dell'autore dell'opera: [Effect of a novel drug-eluted balloon coated with genistein before stent implantation in porcine coronary arteries.Sheiban I, Anselmino M, Moretti C, Biondi-Zoccai G, Galloni M, Vignolini C, Mattoni M, Sciuto F, Omedè P, Trevi GP. Clin Res Cardiol. 2008 Dec;97(12):891-8. doi: 10.1007/s00392-008-0705-2.] The definitive version is available at: La versione definitiva è disponibile alla URL: [http://link.springer.com/article/10.1007%2Fs00392-008-0705-2] Phone: +39-011-6334446. Fax: +39-0116967053. Email: isheiban@yahoo.com 3 Background. The major drawback of stent implantation in native human coronary vessels is the occurrence of restenosis. Drug-eluting stents significantly reduce restenosis after percutaneous coronary intervention (PCI), but may be associated with persistent local inflammation involved in the restenosis mechanisms. In this setting coating coronary devices with anti-inflammatory agents represents an intriguing alternative to stent-based local drug delivery. The aim of the present study was to test in a porcine model the safety and efficacy of a novel Genistein-eluting balloon preceding coronary stenting.Design. Female piglets underwent PCI in a randomized fashion with either a Genistein-eluting or a standard balloon angioplasty, followed in all vessels by bare-metal stent implantation. Pigs were sacrificed at different time points to appraise safety (i.e. endothelialization) and efficacy (i.e. antiinflammatory and anti-proliferative effects): 1, 4, and 6-8 weeks following PCI.Results. Overall analysis was conducted on 14 piglets. Twenty-five bare-metal stents were implanted preceded by angioplasty with a conventional balloon in 13 vessels and by the Genistein-eluted balloon in 12. No untoward effects were reported in either group. Healing and endothelialization appeared universal within four weeks. The Genistein-eluted balloon group disclosed a significant reduction, at four weeks from implantation, of the peri-stent inflammatory cells count (mononucleocytes 39±32 vs.96±29 per square millimetre, p=0.019. This effect did not clearly translate into a trend towards a reduced neointimal hyperplasia at six-eight weeks (0.13±0.11 vs. 0.14±0.09, p=0.835).Conclusion. This study provides the first in vivo demonstration of the anti-inflammatory effects of a Genistein-eluting balloon in PCI, warranting further research including the combination of a Genisteineluting balloon with standard drug-eluting stent.4

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“…1990). However, this rate can readily be reduced simply by premedication with 650 mg aspirin at least 24h before stent implantation (Schwartz et al 1990) underlining the importance of anticoagulation therapies. For testing novel low or non-thrombogenic stent designs and coatings, we feel that implantation conditions should be deliberately made difficult by withholding antithrombotic drugs, anticoagulants, dextran, or antiplatelet aggregation therapies, except for heparin during the implantation procedure.…”

Section: Discussionmentioning

confidence: 99%

Experimental intracoronary stenting: comprehensive experience in a porcine model

et al. 1998

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Appropriate animal models for intracoronary stenting are most important for improving understanding of the pathophysiology of acute occlusion and long-term re-stenosis, which currently limits the safety and efficacy of percutaneous transluminal coronary angioplasty in humans. Since the anatomy and physiology of swine coronary arteries closely resemble those of humans, the procine model should be ideal for testing of stents. This is a comprehensive report on an experimental set-up in pigs, communicating in detail the necessary techniques as well as some modifications facilitating safe intra coronary stent placement and successful follow-up studies for weeks or months. Stent procedure is performed in mechanically ventilated and haemodynamically monitored animals under balanced anaesthesia. Intracoronary application of flow wires allows the assessment of local flow conditions, flow properties and coronary flow reserve. Real-time intravascular ultrasonography (IVUS) provides detailed information on coronary morphology and enables the appropriate sizing of the coronary lumen. From our own experience, we like to propose that the use of the porcine model has the potential to gain new insights into the pathophysiology of intracoronary stent placement-associated complications and allows for the study of modifications in techniques and materials, and the development of novel pharmacological therapeutic strategies.

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Restenosis after balloon angioplasty. A practical proliferative model in porcine coronary arteries.

Cited by 367 publications

References 32 publications

Mouse Model of Femoral Artery Denudation Injury Associated With the Rapid Accumulation of Adhesion Molecules on the Luminal Surface and Recruitment of Neutrophils

Mouse Model of Femoral Artery Denudation Injury Associated With the Rapid Accumulation of Adhesion Molecules on the Luminal Surface and Recruitment of Neutrophils

Effect of a novel drug-eluted balloon coated with Genistein before stent implantation in porcine coronary arteries

Experimental intracoronary stenting: comprehensive experience in a porcine model

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