Resting Membrane Potential and Ionic Distribution in Fast- and Slow-Twitch Mammalian Muscle

Campion, David S.

doi:10.1172/jci107787

Cited by 19 publications

(5 citation statements)

References 17 publications

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“…There was no correlation between pH, and Em at either temperature. The mean values of Em we recorded are in good agreement with those measured with single micro-electrodes: 69-7 mV in guinea-pig soleus (Campion, 1974) and 74 + 8-5 mV in rat soleus (Albuquerque & McIssac, 1969).…”

Section: Measurement Of Phisupporting

confidence: 86%

Micro‐electrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres.

1977

The Journal of Physiology

177

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SUMMARY1. The intracellular pH (pHi) of surface fibres of the mouse soleus muscle has been measured in vitro using recessed-tip pH-sensitive microelectrodes.2. In 5 % CO2 and pH 7 40, the mean pH1 was 7 07 + 0 007 (s.E. of mean) at 370 C and 7-23 + 0.01 at 280 C. The difference between these two values is the same as the change in neutral pH between 37 and 280 C.3. Alteration of the CO2 level at constant external pH caused a biphasic change in pH1 with a rapid displacement followed by a slower partial recovery. Because the recovery was incomplete, different stable pHi values were recorded at different CO2 levels, the higher the CO2 the lower the pHi. The differences in pHi were highly significant both at 37 and 280 C.4. Alteration of the CO2 level at constant external pH also changed the membrane potential (Em), an increase in CO2 leading to an increased Em. The dependence of Em on the CO2 level was much smaller in the fasttwitch muscle, extensor digitorum longus, than in soleus.5. Changing external pH, either by alteration of the bicarbonate or CO2level of the Ringer solution, caused pHi to change by a mean 38-7 % of the external pH change. The change in pHi was accomplished about 10 times more rapidly, and in the same direction, by altering CO2 than by altering the bicarbonate. 6. Application of external NH3 and NH+ caused a rapid intracellular alkalinization followed by a slower acidification. On removal of external NH3 and NH+, there was a large and rapid acidification, followed by a fairly rapid recovery in pHi.7. The size of the pH1 changes occurring on alteration of the CO2 level at both constant external pH and constant external bicarbonate, and on removal of external NH3 and NH+, suggests a non-CO2 buffering

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Section: Measurement Of Phisupporting

confidence: 86%

Micro‐electrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres.

1977

The Journal of Physiology

177

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“…This is in agreement with previous reports on metabolic differences between resting "red" and "white" mammalian skeletal muscle (Ogata 1960, Beatty et al 1%3). Studies on rats (Yonemura 1967) and guinea-pigs (Campion 1974) have shown that "red" muscle fibers have a considerably lower resting membrane potential than "white" fibers. In the present study on cats we were not able to demonstrate any significant difference in the resting membrane potential in the two muscles studied.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study on cats we were not able to demonstrate any significant difference in the resting membrane potential in the two muscles studied. This might be due to differences in the measurement technic or to a species variation (Campion 1974).…”

Section: Discussionmentioning

confidence: 99%

Metabolic responses in feline “red” and “white” skeletal muscle to shock and ischemia

Jennische

Amundson

Haljamäe

1979

Acta Physiologica Scandinavica

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In order to investigate possible differences in the reaction to hypoxic conditions between "red" and "white" skeletal muscle, cats were subjected to a 2 h period of either hemorrhagic shock or hind limb tourniquet ischemia, and the hypoxia induced changes were studied in the soleus and lateral gastrocnemius muscles. Muscle biopsies were analysed for ATP, CP, glucose, G 6-P and lactate. Using microelectrodes, the resting membrane potential was repeatedly measured. Both experimental models resulted in increased tissue lactate levels and a successive decrease in the membrane potential of both muscles studied. No reduction of the high energy phosphagen content (ATP + CP) occurred in any of the muscles during shock. The tourniquet ischemia resulted in a 40% reduction of the ATP + CP content in the soleus muscle, whereas in the gastrocnemius muscle no significant reduction occurred. A significant correlation was found between the tissue lactate content and the membrane potential under both conditions and in both muscles studied. It is concluded that "red" muscles are more susceptible to metabolic derangement than "white" muscles during total ischemia, whereas during hypovolemia "red" muscles appear to be protected from early hypoxic damage, probably due to a redistribution of skeletal muscle blood flow.

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“…There is a well‐established, although not universally acknowledged, relative decline of fast‐twitch type II relative to slow‐twitch type I fibers in human skeletal muscle with aging . Studies in mammalian skeletal muscle have demonstrated relative depolarization of slow‐twitch fibers when compared with their fast‐twitch counterparts . A preponderance of relatively depolarized type I fibers in the recordings from older vs younger subjects in our cohort is an alternative explanation for our findings.…”

Section: Discussionmentioning

confidence: 48%

“…17,46,47 Studies in mammalian skeletal muscle have demonstrated relative depolarization of slow-twitch fibers when compared with their fast-twitch counterparts. 48,49 A preponderance of relatively depolarized type I fibers in the recordings from older vs younger subjects in our cohort is an alternative explanation for our findings.…”

Section: Discussionmentioning

confidence: 62%

Sarcolemmal excitability changes in normal human aging

Lee

Boland-Freitas

2018

Muscle and Nerve

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Resting Membrane Potential and Ionic Distribution in Fast- and Slow-Twitch Mammalian Muscle

Cited by 19 publications

References 17 publications

Micro‐electrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres.

Micro‐electrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres.

Metabolic responses in feline “red” and “white” skeletal muscle to shock and ischemia

Sarcolemmal excitability changes in normal human aging

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