Restoration of critical defects in the rabbit mandible using osteoblasts and vascular endothelial cells co-cultured with vascular stent-loaded nano-composite scaffolds

Xu, Hong zhen; Su, Jian

doi:10.1016/j.jmbbm.2021.104831

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Furthermore, Nano-scaffolds should promote cell adhesion, proliferation, and differentiation, facilitate controlled release of bioactive molecules for enhanced cell behavior and tissue regeneration, and have a high surface area-to-volume ratio allowing extensive cell-scaffold interaction [49][50][51][52][53][54].These characteristics are achieved by mimicking the natural ECM of neural tissue, which provides a supportive environment for cell growth and function [41,51,55]. Our data demonstrated that initial cell adhesion, migration, and differentiation of NSCs were signi cantly improved when encapsulated in the Nano-SDF compared to the R-GSIK.…”

Section: Discussionmentioning

confidence: 99%

Nano-Scaffold Containing a Functional Motif of Stromal Cell-Derived Factor 1 Enhances Neural Stem Cell Behavior and Promotes Synaptogenesis in a Traumatic Brain Injury Model

Tork,

Saberifar,

Yekta

et al. 2024

Preprint

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Traumatic brain injury (TBI) is a leading cause of mortality and morbidity worldwide, presenting a significant challenge due to the lack of effective therapies. Neural stem cells (NSCs) have shown promising potential in preclinical studies as a therapy for TBI. However, their application is limited by challenges related to poor survival and integration within the injured brain. In this study, we investigated the effect of a novel nano-scaffold containing stromal cell-derived factor 1 (SDF-1) on NSC behavior and synaptogenesis after TBI. Using an innovative design, we successfully fabricated a nano-scaffold with Young's modulus of approximately 3.21 kPa, which aligns closely with the mechanical properties exhibited by neural tissue. This achievement marks the first time such a scaffold has been created and has promising implications for its potential use in neural tissue engineering applications. Our findings demonstrate that the nano-scaffold enhances NSC proliferation, migration, and differentiation capacity in vitro. Moreover, when transplanted into the injured brain, the nano-scaffold promotes the survival and integration of NSCs, leading to increased synaptogenesis and functional recovery. These findings suggest that the use of the novel nano-scaffold containing SDF-1 could provide a promising approach to the treatment of TBI by improving NSC behavior and promoting synaptogenesis.

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Section: Discussionmentioning

confidence: 99%

Nano-Scaffold Containing a Functional Motif of Stromal Cell-Derived Factor 1 Enhances Neural Stem Cell Behavior and Promotes Synaptogenesis in a Traumatic Brain Injury Model

Tork,

Saberifar,

Yekta

et al. 2024

Preprint

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“…Te surface of both endothelial cells and osteoblasts expresses vascular endothelial growth factor receptor 2 (VEGFR2), which integrates the processes of bone formation and angiogenesis [28,30]. It had been reported that human umbilical vein endothelial cells (HUVECs) could stimulate osteoblast proliferation and vascular endothelial cells were usually combined with osteoblasts or MSC according to many researches of bone repair [31,32]. Although the synergistic efects of osteogenesis and angiogenesis have been confrmed in many studies [26,33,34], while little research has focused on the therapeutic potential of exosomes released by endothelial cells for SNFH.…”

Section: Discussionmentioning

confidence: 99%

Endothelial Cell-Derived Exosomes Inhibit Osteoblast Apoptosis and Steroid-Induced Necrosis of Femoral Head Progression by Activating the PI3K/Akt/Bcl-2 Pathway

Sun,

Yao,

Luo

et al. 2024

Journal of Tissue Engineering and Regenerative Medicine

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The aim of the study was to investigate the therapeutic potential of exosomes secreted by endothelial cells (EC-exos) on steroid-induced osteonecrosis of femoral head (SNFH). First, we successfully obtained EC-exos through differential centrifugation. Then, the effects of EC-exos on mouse embryo osteoblast precursor (MC3T3-E1) cells under high concentration of dexamethasone (Dex) were analysed in vitro, which included cell migration, viability, and apoptosis. In vivo, a SNFH rat model was successfully established and treated with EC-exos. Micro-computed tomography (micro-CT) and haematoxylin and eosin (H&E) were used to observe femoral trabeculae. Our in vitro results showed that EC-exos improved cell viability and migration of osteoblasts and reduced the apoptotic effect of high concentration of Dex on osteoblasts in vitro. Phosphoinositide 3-kinase (PI3K)/Akt/Bcl-2 signalling pathway was activated in MC3T3-E1 cells under the response to EC-exos. In vivo, increased bone volume per tissue volume (BV/TV) p=0.031, trabecular thickness (Tb.Th) p=0.020, and decreased separation (Tb.Sp) p=0.040 were observed in SNFH rats treated with EC-exos. H&E staining revealed fewer empty lacunae and pyknotic osteocytes in trabeculae. The expression of Bcl-2 and Akt in EC-exos group was significantly increased in trabeculae tissue. Overall, our finding indicated that EC-exos could attenuate SNFH by inhibiting osteoblast apoptosis via the PI3K/Akt/Bcl-2 pathway.

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“…Three-dimensional fibrous SiO 2 nanofibers with chitosan as bonding sites (SiO 2 NF-CS) scaffolds were developed by electrospinning and lyophilization technique, the in vivo experiments demonstrated that SiO 2 NF-CS scaffolds could fill the mandibular defect in rabbits [ 117 ]. The poly-caprolactone/nano-hydroxyapatite/beta-calcium phosphate (PCL/nHA/β-TCP) composite scaffolds loaded with poly-(lactic-co-glycolic acid)/nano-hydroxyapatite/collagen/heparin sodium (PLGA/nHA/Col/HS) small vascular stents were sufficient to repair critical defects, which were more than 25 mm in rabbit mandibles [ 118 ]. Biocompatible and osteoinductive electrospun nanomaterials have shown great potential in jaw defect repair.…”

Section: Literature Reviewmentioning

confidence: 99%

A Bibliometric Analysis of Electrospun Nanofibers for Dentistry

Jin

Yeung

Zhang

et al. 2022

JFB

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Electrospun nanofibers have been widely used in dentistry due to their excellent properties, such as high surface area and high porosity, this bibliometric study aimed to review the application fields, research status, and development trends of electrospun nanofibers in different fields of dentistry in recent years. All of the data were obtained from the Web of Science from 2004 to 2021. Origin, Microsoft Excel, VOSviewer, and Carrot2 were used to process, analyze, and evaluate the publication year, countries/region, affiliations, authors, citations, keywords, and journal data. After being refined by the year of publication, document types and research fields, a total of 378 publications were included in this study, and an increasing number of publications was evident. Through linear regression calculations, it is predicted that the number of published articles in 2022 will be 66. The most published journal about electrospun dental materials is Materials Science & Engineering C-Materials for Biological Applications, among the six core journals identified, the percent of journals with Journal Citation Reports (JCR) Q1 was 60%. A total of 17.60% of the publications originated from China, and the most productive institution was the University of Sheffield. Among all the 1949 authors, the most productive author was Marco C. Bottino. Most electrospun dental nanofibers are used in periodontal regeneration, and Polycaprolactone (PCL) is the most frequently used material in all studies. With the global upsurge in research on electrospun dental materials, bone regeneration, tissue regeneration, and cell differentiation and proliferation will still be the research hotspots of electrospun dental materials in recent years. Extensive collaboration and citations among authors, institutions and countries will also reach a new level.

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Restoration of critical defects in the rabbit mandible using osteoblasts and vascular endothelial cells co-cultured with vascular stent-loaded nano-composite scaffolds

Cited by 7 publications

References 43 publications

Nano-Scaffold Containing a Functional Motif of Stromal Cell-Derived Factor 1 Enhances Neural Stem Cell Behavior and Promotes Synaptogenesis in a Traumatic Brain Injury Model

Nano-Scaffold Containing a Functional Motif of Stromal Cell-Derived Factor 1 Enhances Neural Stem Cell Behavior and Promotes Synaptogenesis in a Traumatic Brain Injury Model

Endothelial Cell-Derived Exosomes Inhibit Osteoblast Apoptosis and Steroid-Induced Necrosis of Femoral Head Progression by Activating the PI3K/Akt/Bcl-2 Pathway

A Bibliometric Analysis of Electrospun Nanofibers for Dentistry

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