Restoration of Endocrine Function and Fertility With a Tubo-Ovarian Autotransplant as the Anatomical-Functional Unit in Rabbits Using a Vascular Microsurgical Technique

Meraz, M.M.; Revilla, C; Martinez, C; Islas-Andrade, S.; Aburto, Enrique

doi:10.1016/j.transproceed.2006.02.041

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…Transplant of the ovary and/or fallopian tube or of the uterus, with the objective of re-establishing fertility and/or ovarian endocrine function, has been achieved in different species such as guinea pig, hamster, rat, rabbit, dog, sheep, pig, primate, and in women, with varying results (14)(15)(16)(17)(18)(19)(21)(22)(23)(24)(25)(26)(27)(28). In women, fallopian tube transplant has been performed unsuccessfully in 21 cases with the objective of re-establishing fertility.…”

Section: Discussionmentioning

confidence: 99%

“…Following this, both vessels were divided below each previously placed ligature, and tubal-ovarian graft was isolated from its connection to fatty and peritoneal tissue ( Figure 1). The catheterized graft was placed on wet gauze and carried to a container containing "whipped" ice from the same perfusion solution prepared at 4 • C [14]. Finally, the graft was passed on to the second team to begin "bench surgery."…”

Section: Donor Rabbitmentioning

confidence: 99%

“…Tubal-ovarian adhesions were intentionally searched for microscopically. For their evaluation, the same classification described in another study was used [14]. HTA plus RSO was performed for histological study and rabbits were then euthanized.…”

Section: Postoperative Evaluationmentioning

confidence: 99%

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Restoration of Endocrine Function and Fertility with Orthotopic Tubal—Ovarian Allotransplant as the Anatomical-Functional Unit in Rabbits

Meraz

Juárez

Revilla-Monsalve

et al. 2008

Journal of Investigative Surgery

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The aim of this study was to re-establish endocrine and reproductive function in tubal-ovariectomized rabbits using orthotopic tubal-ovary allotransplants (OT-OA). Fifty-five New Zealand White nonconsanguineous rabbits were used and allocated into five experimental groups: Each group comprised donors submitted to right salpingo-oophorectomy that served as the donated allograft. In group 1 no cyclosporin (CsA) was administered and rabbits were submitted to left salpingo-oophorectomy (LSO). Group 2 was the allotransplant group and did not receive CsA. Group 3 was the allotransplant group with CsA. They were submitted to bilateral salpingo-oophorectomy followed by OT-OA. CsA of 10 mg/kg/day was administered to rabbits for the first 21 days and 5 mg/kg/day for the remainder of the study. Group 4 received CsA just as for group 3 and was submitted to LSO. During the study the following were measured: estradiol (E2), progesterone (P4), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and histopathological study of the uteri, tubes, and ovaries. Groups 1, 3, and 4 showed a significant increase in serum E2 and P4 levels corresponding to the second week of gestation, compared to group 2 (p < .05). Group 2 showed a significant increase in serum LH and FSH levels during week 10 compared to serum LH and FSH levels in groups 1, 3, and 4 (p < .05). In group 3, three rabbits did not reject the allotransplant, one rabbit became pregnant, and endocrine function was re-established in two rabbits. It can be concluded that OT-OA together with CsA administration re-establishes endocrine function and fertility.

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Section: Discussionmentioning

confidence: 99%

Section: Donor Rabbitmentioning

confidence: 99%

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Restoration of Endocrine Function and Fertility with Orthotopic Tubal—Ovarian Allotransplant as the Anatomical-Functional Unit in Rabbits

Meraz

Juárez

Revilla-Monsalve

et al. 2008

Journal of Investigative Surgery

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“…Vascular transplantation of fresh whole ovaries has been largely successful in animal models (rats [ 103 , 104 , 105 ], rabbits [ 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ], sheep [ 47 , 49 , 67 , 68 , 78 , 115 , 116 , 117 , 118 , 119 , 120 , 121 ], pigs [ 122 , 123 ], dogs [ 124 ] and monkeys [ 125 , 126 ]). Encouraging results have also been reported on transplantation of frozen–thawed whole ovaries in rats [ 66 , 127 , 128 , 129 ], rabbits [ 130 ] and sheep [ 38 , 40 , 41 , 48 , 49 , 50 , 67 , 68 , 78 , 95 , 102 , 131 ], which will be detailed further in this review.…”

Section: Challenges and Solutionsmentioning

confidence: 99%

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans

Hossay

Donnez²,

Dolmans

2020

JCM

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Ovarian tissue cryopreservation and transplantation is the only fertility preservation option that enables both restoration of fertility and resumption of ovarian endocrine function, avoiding the morbidity associated with premature menopause. It is also the only technique available to prepubertal patients and those whose treatment cannot be delayed for life-threatening reasons. Ovarian tissue cryopreservation can be carried out in two different ways, either as ovarian cortical fragments or as a whole organ with its vascular pedicle. Although use of cortical strips is the only procedure that has been approved by the American Society for Reproductive Medicine, it is fraught with drawbacks, the major one being serious follicle loss occurring after avascular transplantation due to prolonged warm ischemia. Whole ovary cryopreservation involves vascular transplantation, which could theoretically counteract the latter phenomenon and markedly improve follicle survival. In theory, this technique should maintain endocrine and reproductive functions much longer than grafting of ovarian cortical fragments. However, this procedure includes a number of critical steps related to (A) the level of surgical expertise required to accomplish retrieval of a whole ovary with its vascular pedicle, (B) the choice of cryopreservation technique for freezing of the intact organ, and (C) successful execution of functional vascular reanastomosis upon thawing. The aim of this systematic review is to shed light on these challenges and summarize solutions that have been proposed so far in animal experiments and humans in the field of whole ovary cryopreservation and transplantation.

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“…Nuestro grupo de investigación ha llamado al injerto tubo-ovárico "unidad anatomo-funcional" y hemos trasplantado el ovario con su pedículo vascular de vena y arteria junto con la trompa de Falopio hasta su porción ístmica, reestableciendo la función endocrina y de fertilidad posterior a autotrasplante tubo-ovárico en conejas (94).…”

Section: Justificaciónunclassified

Alotrasplante tubo-ovárico como "unidad anatomo-funcional" en la coneja

Meraz

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Restoration of Endocrine Function and Fertility With a Tubo-Ovarian Autotransplant as the Anatomical-Functional Unit in Rabbits Using a Vascular Microsurgical Technique

Cited by 4 publications

References 16 publications

Restoration of Endocrine Function and Fertility with Orthotopic Tubal—Ovarian Allotransplant as the Anatomical-Functional Unit in Rabbits

Restoration of Endocrine Function and Fertility with Orthotopic Tubal—Ovarian Allotransplant as the Anatomical-Functional Unit in Rabbits

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans

Alotrasplante tubo-ovárico como "unidad anatomo-funcional" en la coneja

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