Restoration of MPTP-induced deficits by exercise and Milmed^®co-treatment

Abstract

1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces permanent neurochemical and functional deficits. Following the administration of either two or four injections of the dopamine neurotoxin, MPTP, at a dose of 40 mg/kg, C57/BL6 mice were given access to running-wheels (30-min sessions, four times/week, Monday–Thursday) and treatment with the treated yeast, Milmed® (four times/week, Monday–Thursday), or simply running-wheel exercise by itself, over ten weeks. It was observed that the combination of physical exercise and Milmed® treatment, the MPTP + Exercise + Yeast (MC) group [MPTP + Exercise + Milmed® (MC)], restored spontaneous motor activity markedly by test day 10, restored completely subthreshold L-Dopa-induced activity, and dopamine concentration to 76% of control values, in the condition wherein two administrations of MPTP (2 × 40 mg/kg) were given prior to initiation of exercise and/or Milmed® treatment. Physical exercise by itself, MPTP + Exercise (MC) group, attenuated these deficits only partially. Administration of MPTP four times (i.e., 40 mg/kg, s.c., once weekly over four weeks for a total of 160 mg/kg, MPTP + Exercise + Yeast (MC) group [MPTP + Exercise + Milmed® (SC)] and MPTP + Exercise (SC), induced a lesioning effect that was far too severe for either exercise alone or the exercise + Milmed® combination to ameliorate. Nevertheless, these findings indicate a powerful effect of physical exercise reinforced by Milmed® treatment in restoring MPTP-induced deficits of motor function and dopamine neurochemistry in mice.

Amelioration For Allergic Rhinitis and Asthma: The Treated Yeast Milmed and Physical Exercise

“…There were significant improvements in fractional oral exhaled nitric oxide accompanied by a significant decrease iron nitrate, and improvements in nasal eosinophilic cell counts due to exercise that prompted the conclusion of sustainable improvements in allergic symptoms. It has been demonstrated repeatedly that physical exercise combined with Milmed treatment over six to fourteen weeks induced a synergistic effect against symptoms of dopamine neurodegeneration [11,12]. The present account promotes the contention the physical exercise with accompanying Milmed treatment may offer a novel and viable alternative to anti-allergy interventions under current usage.…”

Milmed Yeast Alters the LPS-Induced M1 Microglia Cells to Form M2 Anti-Inflammatory Phenotype

“…The present results imply that the Milmed preparation of S. cerevisiae, treated with electromagnetic wavelengths in the Extreme High Frequency—millimeter range [ 2 , 22 , 28 , 56 ], present a probiotic agent that drives the innate immune cells’ polarization disposition. According to this scenario, the decrease in cytokine release after LPS inflammatory stimulus and the polarization of microglial cells towards an anti-inflammatory phenotype, as confirmed by the induction of IL-10 and arginase-1, following Milmed addition, leads to the assertion that Milmed supplementation offers a promising treatment for pathologies arising from neuro-inflammation, such as depression, Parkinsonism and AD.…”

Milmed Treatment Alleviates Symptoms of Allergy and Improves General Health

“…Probiotics are live organisms present in foods, which yield health benefits related to their interactions with the gastrointestinal tract. In the MPTP-mouse model of Parkinsonism [24,25], it was observed the milmed-physical exercise combination restored repeatedly both functional motor activity and dopamine concentrations, as well as increasing markedly the levels of brain-derived neurotrophic factor (BDNF) in motor cortex regions. The antiinflammatory effects of probiotic therapies have been documented [32,33].…”

“…It has been observed that specific biological and therapeutic effects of Extremely High Frequency (EHF) electromagnetic fields may be transferred to living organisms by specially treated suspension of living yeast cells [22,18]. The treatment of yeast cell cultures themselves, as the ''transferenceagent'' provides an agent that has been shown provide both anti-parkinson and anti-neurodegenerative effect [23][24][25] in addition to an anti-allergy effect [26]. The treatment and preparation of S. cerevisiae or S. carlsbergsis with electromagnetic waves in the Extreme High Frequency (EHF) range of 30-300 GHz produces a treated yeast extract, given the name Milmed (i.e.…”