Restoration of X-irradiated L-cells by Means of Highly Polymerized Isologous Deoxyribonucleic Acid

Miletić, B; Petrović, Danilo; Zajec, L

doi:10.1038/197090a0

Cited by 19 publications

(5 citation statements)

References 2 publications

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“…Previously, it has been reported that UV-damaged DNA fragments provide a cytoprotective effect to mammalian cells [3,4,[7][8][9]11]. Here, we report on the first observations that UV-damaged mRNA fragments have a similar property.…”

Section: Discussionsupporting

confidence: 59%

“…This may explain the early observations of a protective effect of DNA in other systems [3,4,7,8,9,11]. Djord- 13 jecic et al [7] and Miletic et al [8] were the first to report the enhancement of cell survival after UV and X irradiation with external DNA treatments. This work was later confirmed by Horikawa et al [11] using cellular fractions and DNA.…”

Section: Introductionmentioning

confidence: 78%

“…If this response is present in mammalian cells it may be precipitated by the addition of exogenous, damaged DNA [3,4]. This may explain the early observations of a protective effect of DNA in other systems [3,4,7,8,9,11]. Djord- 13 jecic et al [7] and Miletic et al [8] were the first to report the enhancement of cell survival after UV and X irradiation with external DNA treatments.…”

Section: Introductionmentioning

confidence: 86%

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Normal Human Keratinocytes Pretreated with Damaged mRNA Are Resistant to UVB Damage and Exhibit Increased DNA Repair

Mammone

Goyarts

et al. 2004

Skin Pharmacol Physiol

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Treatment of normal human keratinocytes with UVC-irradiated rabbit globin mRNA 24 h before and after UVB exposure increased the survival of the human keratinocytes. We also observed that UVC-damaged mRNA reduced the formation of sunburn cells in skin models. We next tested the effects of UVC-damaged mRNA on cellular repair of DNA. DNA repair was evaluated using 2 assay methods. The first method used a damaged plasmid that is transfected back into the cell where it is repaired by the host cell repair mechanism. In these experiments, we observed that externally added UVC-damaged rabbit globin mRNA enhanced the repair of a plasmid transfected into the host keratinocyte cells. The second method used to determine the effects on DNA repair was direct immunostaining for thymidine-thymidine dimers (TT dimers) in histological sections of the skin models. Skin models were irradiated with UVB and then fixed immediately or after 24 h and stained for TT dimers. UVB irradiation immediately caused an increase in the number of stained keratinocytes in the skin. The number of stained cells decreased in skin fixed 24 h after UVB. This is due to repair of the TT dimers and their removal. Sections of skin models pretreated with UV-damaged mRNA exhibit greater removal of these TT dimers after 24 h. The above evidence suggests that damaged mRNA can trigger a host cell DNA repair pathway.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 86%

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Normal Human Keratinocytes Pretreated with Damaged mRNA Are Resistant to UVB Damage and Exhibit Increased DNA Repair

Mammone

Goyarts

et al. 2004

Skin Pharmacol Physiol

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“…The recovery of cell growth after ultraviolet and x-irradiation of mouse L cells by treatment with DNA fragments was reported over 30 years ago by Djordeic et al [6] and later Miletic et al [7]. These authors discovered that DNA, independent of its source, could aid in the recovery of irradiated mouse L cells.…”

Section: Resultsmentioning

confidence: 86%

“…This may explain the early observations of a protective effect of DNA in other systems. Djordjecic et al [6] and Miletic et al [7] were the first to report the enhancement of cell survival after UV and X-irradiation with DNA treatments. This work was later confirmed by Horikawa et al [8] using cellular fractions and deoxyribonucleic acid.…”

Section: Introductionmentioning

confidence: 99%

The Cytoprotective Effects of Exogenous DNA Fragments

Mammone

Ingrassia²,

Gan³

et al. 2001

Skin Pharmacol Physiol

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Ultraviolet irradiation of normal human keratinocytes induces a cytotoxic effect. The chromophore for this effect is believed to be genomic DNA. However, DNA damage is known to be repaired in UVB irradiated keratinocytes. The trigger for this DNA repair is potentially damaged DNA itself. To test the hypothesis that damaged DNA can induce the host cell’s own DNA repair mechanism, we treated the keratinocytes with the damaged DNA and evaluated its cytoprotective effects. We have observed that fragmented calf thymus DNA irradiated and damaged with a UVC light can induce a protective effect in cultured human keratinocytes. Keratinocytes treated with UVC damaged DNA fragments are less susceptible to UVB irradiation-induced cell death as measured by neutral red uptake. Unirradiated exogenous DNA did not induce this protective effect. Similar protective effects can be seen with irradiated salmon sperm DNA. UVC damaged DNA fragments induced 60% increase in protection in human HaCaT keratinocyte in culture to the cell death induced by UVB. Similar protection was observed with UVC irradiated oligothymidylic acid (dT3-dT5) which increased the survival of human HaCaT keratinocytes after UVB irradiated by 50%. Isolated mononucleotides, irradiated or not, do not increase UVB survivability. Cellular DNA synthesis was greatly inhibited by UVB, becoming undetectable at 40 mJ/cm². Exogenous treatment with damaged fragments causes immediate and significant inhibition of total cellular DNA synthesis. This inhibition was dose dependent. Cells that undergo damage to their DNA are known to inhibit endogenous DNA synthesis via p53 suppressor gene activation. This is believed to allow them sufficient time to repair the host DNA. The cellular response to exogenous damaged DNA may be a similar mechanism.

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Restoration of Immunologic Capacities in Irradiated Animals by Nucleic Acids and their Derivatives

Simić

Kanazir

1968

Nucleic Acids in Immunology

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Restoration of X-irradiated L-cells by Means of Highly Polymerized Isologous Deoxyribonucleic Acid

Cited by 19 publications

References 2 publications

Normal Human Keratinocytes Pretreated with Damaged mRNA Are Resistant to UVB Damage and Exhibit Increased DNA Repair

Normal Human Keratinocytes Pretreated with Damaged mRNA Are Resistant to UVB Damage and Exhibit Increased DNA Repair

The Cytoprotective Effects of Exogenous DNA Fragments

Restoration of Immunologic Capacities in Irradiated Animals by Nucleic Acids and their Derivatives

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